CAJ | 학술논문

应用AFLP和HPLC方法研究了云南红豆杉(Taxus wallichiana var.wallichiana)的遗传多样性与紫杉醇含量之间的关系.AFLP指纹图谱显示7个居群谱带明显分为两种式样.PopGene1.31和Arlequin3.1软件分析结果表明:云南红豆杉居群内遗传比较稳定,居群间遗传分化极其显著(F_(st)=0.67).测定当年生小枝叶紫杉醇含量结果表明:紫杉醇含量在居群间和居群内个体差异都较大.天然林中潞西居群的紫杉醇平均含量为0.0185%;腾冲和永德居群的紫杉醇含量比较低,分别为0.0049%和0.0087%;禄丰栽培居群紫杉醇含量比较高,平均为0.0225%.在居群水平上,遗传多样性和紫杉醇含量有一定程度的关联,但在个体水平上明显无对应关系.
응용AFLP화HPLC방법연구료운남홍두삼(Taxus wallichiana var.wallichiana)적유전다양성여자삼순함량지간적관계.AFLP지문도보현시7개거군보대명현분위량충식양.PopGene1.31화Arlequin3.1연건분석결과표명:운남홍두삼거군내유전비교은정,거군간유전분화겁기현저(F_(st)=0.67).측정당년생소지협자삼순함량결과표명:자삼순함량재거군간화거군내개체차이도교대.천연림중로서거군적자삼순평균함량위0.0185%;등충화영덕거군적자삼순함량비교저,분별위0.0049%화0.0087%;록봉재배거군자삼순함량비교고,평균위0.0225%.재거군수평상,유전다양성화자삼순함량유일정정도적관련,단재개체수평상명현무대응관계.
We studied the relationship between genetic divemity and taxol content of Taxus wallichiana vat.wallichiana us-ing AFLP and HPLC.The results of AFLP fingerprinting indicated that two patterns were discovered among Seven popula-tions.Through PopGene 1.31 and Adequin 3.1 software,stable genetics within population and significant differentiation among populations(F_(st)=0.67)were revealed.However,taxol content was markedly different within and among popula-tions when testing the one-yeartwigs.Among wild forests,the highest taxol content was found in Luxi population which av-erage percentage wag 0.0185%.While the taxol content level Wag low in Tengchong and Yongde populations,which were 0.0049% and 0.0087% respectively.The cultivated population in Lufeng showed the highest taxol content level which was 0.0225% comparing with that in the populatiom of wild forests.There were relations between genetic diversity and taxol content at the population level to some extent,while no corresponding relations were found at the individual level.