CAJ | 학술논문

目的:对产邻苯二酚菌株进行筛选.方法:采用前期筛得的产邻苯二酚菌为出发菌株,通过硫酸二乙酯诱变的方法使该菌株突变,同时建立96孔板培养和酶标仪检测方法对产邻苯二酚菌株进行高通量筛选.结果:硫酸二乙酯的终浓度为0.1%,诱变时间为8 min的条件下,突变菌致死率为84.5%,突变效果最好.筛选培养基中吸光值(495nm)和富集培养基中菌液浊度值(OD_(630))的加和值较大的突变菌株产邻苯二酚能力高.通过诱变和筛选得到的菌株,产邻苯二酚浓度可达0.87mg/ml,比出发菌株的提高了262.5%.经过形态学和生理生化反应,初步鉴定该菌株属于假单胞菌属(Pseudomonas sp.).结论:硫酸二乙酯诱变和96孔板筛选的方法能以高通量方式快速筛选出产邻苯二酚菌株.
목적:대산린분이분균주진행사선.방법:채용전기사득적산린분이분균위출발균주,통과류산이을지유변적방법사해균주돌변,동시건립96공판배양화매표의검측방법대산린분이분균주진행고통량사선.결과:류산이을지적종농도위0.1%,유변시간위8 min적조건하,돌변균치사솔위84.5%,돌변효과최호.사선배양기중흡광치(495nm)화부집배양기중균액탁도치(OD_(630))적가화치교대적돌변균주산린분이분능력고.통과유변화사선득도적균주,산린분이분농도가체0.87mg/ml,비출발균주적제고료262.5%.경과형태학화생리생화반응,초보감정해균주속우가단포균속(Pseudomonas sp.).결론:류산이을지유변화96공판사선적방법능이고통량방식쾌속사선출산린분이분균주.
Objective: Screen of strains producing catechol. Method: Strains producing catechol were screened using diethyl sulphate as mutageniz-ing reagent and 96 well plate screening method. Result: It showed that mutagenizing reagent content at ratio of 0.1 %, at treatment time of 8min were suitable conditions for mutagenesis. Under specific conditions, the lethal rate was 84.5% .The mutant strains which showed high absorbance value in 495nm in sceening fermentation medium and high OD_(630) in enrichment medium would be better candidate for catechol-producing. Though mutagenizing and plate selection, the catechol yield of the strian was 0.87rng/ml, increasing by 262.5% compared to the original one. The strain was preliminary identified as Pseudomonas sp. by means of morphological, physiological and biochemical identification. Conclusion: Catechol producing strains could rapidly screened by high throughout screening using diethyl sulphate as mutagenizing reagent and 96 well plate screening method.