CAJ | 학술논문

目的研究胰腺癌细胞线粒体DNA调控序列(D-loop)区突变情况.方法用PCR与直接测序相结合的方法,对比分析二株胰腺癌细胞株SW1990,JF-305和1例原代培养的正常胰腺细胞的线粒体DNA D-loop区的突变位点.结果二株胰腺癌细胞系和正常胰腺细胞的线粒体DNA D-loop区均存在不同程度的点突变,SW1990共检测到8个突变位点,JF-305共检测9个突变位点,其中73位A-G,16223位C-T,16358位C-T这三个突变位点在二株癌细胞和正常胰腺细胞中均检测到,考虑为多态性变化,其中16211位C-T,16311位T-C两个相同的突变位点在二株胰腺癌细胞中均检测到,考虑为特征性突变.结论胰腺癌细胞线粒体DNA D-loop区具有多态性和突变性,其特征性突变有望成为新的分子标记.
목적 연구이선암세포선립체DNA조공서렬(D-loop)구돌변정황.방법 용PCR여직접측서상결합적방법,대비분석이주이선암세포주SW1990,JF-305화1례원대배양적정상이선세포적선립체DNA D-loop구적돌변위점.결과 이주이선암세포계화정상이선세포적선립체DNA D-loop구균존재불동정도적점돌변,SW1990공검측도8개돌변위점,JF-305공검측9개돌변위점,기중73위A-G,16223위C-T,16358위C-T저삼개돌변위점재이주암세포화정상이선세포중균검측도,고필위다태성변화,기중16211위C-T,16311위T-C량개상동적돌변위점재이주이선암세포중균검측도,고필위특정성돌변.결론 이선암세포선립체DNA D-loop구구유다태성화돌변성,기특정성돌변유망성위신적분자표기.
Objective To explore the mutation situation of pancreatic cancer cell mitochondria DNA D-loop region. Method PCR and direct sequencing was used to analyze the mutational site of mitochondria DNA D-loop region in two pancreatic cancer cell lines SW1990 and JF-305 and normal primary cultured pancreas cell. Result The point mutations were found in two pancreatic cancer cell lines and normal primary cultured pancreas cell. Eight point mutations were found in SW1990 and 9 point mutations were found in JF-305. Three point mutations (73 site A-G,16223 site C-T, and 16358 site c-T) existed in all two pancreatic cancer cell lines and normal primary cultured pancreas cells, which can be considered as polymorphism. Other two point mutations (16211 site C-T and 16311 site T-C) were only found in two pancreatic cancer cell lines, which can be considered as special mutations. Conclusion The mitochondrial DNA D-loop region of pancreatic cancer cells existed polymorphism and special mutations, and the special mutations might be new molecule marker.