中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2008年
4期
201-203
,共3页
刘法兵%刘永%刘志宏%范昱%包尔敦%邱建新%谭建明
劉法兵%劉永%劉誌宏%範昱%包爾敦%邱建新%譚建明
류법병%류영%류지굉%범욱%포이돈%구건신%담건명
吡咯烷类%移植物闭塞%血管%NF-κB
吡咯烷類%移植物閉塞%血管%NF-κB
필각완류%이식물폐새%혈관%NF-κB
Pyrrolidines%Graft occlusion,vascular%NF-κB
目的 研究核转录因子-κB的抑制剂吡咯烷二硫代氨基甲酸酯(PDTC)对慢性移植物血管病的抑制作用.方法 以Lewis大鼠为供者,F344大鼠为受者,建立心脏移植模型.随机将受者分为2组,每组18只.对照组:自术日起腹腔注射环孢素A(CsA)2 mg·kg-1·d-1×10 d;试验组:自术日起除腹腔注射CsA 2 mg·kg-1·d-1×10 d外,另皮下注射PDTC 100 mg·kg-1·d-1×10 d.两组于术后20、40、60 d时分别随机处死受者各6只,取移植心行病理学检查.观察两组冠状动脉内膜的增生程度以及单核细胞的浸润程度;对两组移植心的冠状动脉重塑和炎症反应情况进行评分.结果 在各检查时段,试验组移植心冠状动脉内膜的增生程度与对照组相比明显减轻,差异均有统计学意义(P＜0.05).在术后20 d时,试验组移植心单核细胞的浸润程度与对照组相比明显减轻,差异有统计学意义(P＜0.05).结论 PDTC对大鼠移植物慢性移植物血管病的进程有明显抑制作用.
目的 研究覈轉錄因子-κB的抑製劑吡咯烷二硫代氨基甲痠酯(PDTC)對慢性移植物血管病的抑製作用.方法 以Lewis大鼠為供者,F344大鼠為受者,建立心髒移植模型.隨機將受者分為2組,每組18隻.對照組:自術日起腹腔註射環孢素A(CsA)2 mg·kg-1·d-1×10 d;試驗組:自術日起除腹腔註射CsA 2 mg·kg-1·d-1×10 d外,另皮下註射PDTC 100 mg·kg-1·d-1×10 d.兩組于術後20、40、60 d時分彆隨機處死受者各6隻,取移植心行病理學檢查.觀察兩組冠狀動脈內膜的增生程度以及單覈細胞的浸潤程度;對兩組移植心的冠狀動脈重塑和炎癥反應情況進行評分.結果 在各檢查時段,試驗組移植心冠狀動脈內膜的增生程度與對照組相比明顯減輕,差異均有統計學意義(P＜0.05).在術後20 d時,試驗組移植心單覈細胞的浸潤程度與對照組相比明顯減輕,差異有統計學意義(P＜0.05).結論 PDTC對大鼠移植物慢性移植物血管病的進程有明顯抑製作用.
목적 연구핵전록인자-κB적억제제필각완이류대안기갑산지(PDTC)대만성이식물혈관병적억제작용.방법 이Lewis대서위공자,F344대서위수자,건립심장이식모형.수궤장수자분위2조,매조18지.대조조:자술일기복강주사배포소A(CsA)2 mg·kg-1·d-1×10 d;시험조:자술일기제복강주사CsA 2 mg·kg-1·d-1×10 d외,령피하주사PDTC 100 mg·kg-1·d-1×10 d.량조우술후20、40、60 d시분별수궤처사수자각6지,취이식심행병이학검사.관찰량조관상동맥내막적증생정도이급단핵세포적침윤정도;대량조이식심적관상동맥중소화염증반응정황진행평분.결과 재각검사시단,시험조이식심관상동맥내막적증생정도여대조조상비명현감경,차이균유통계학의의(P＜0.05).재술후20 d시,시험조이식심단핵세포적침윤정도여대조조상비명현감경,차이유통계학의의(P＜0.05).결론 PDTC대대서이식물만성이식물혈관병적진정유명현억제작용.
Objective To investigate the effects of pyrrolidine dithiocarbamate(PDTC),the special inhibitor of nuclear transcription factor-κB,on the progress of chronic transplant vasculopathy (CTV). Methods Heterotopic heart transplantation models were established from Lewis to F344 rats. The recipients were randomly divided into 2 groups(n=18 in each group). In control group,the PDTC group，recipients were injected subcutaneously with PDTC(100 mg·kg-1·day-1)combined
with CsA(2 mg·kg-1·day-1)for 10 days continuously. On the 20th. 40th and 60th day after trans plantation,6 grafts were harvested respectively for histological examination in each group. The prolif eration of coronary artery intima and mononuclear cell infiltration in allografts were assessed. The pathological scores of CTV were evaluated on vascular remodeling and local inflammation. Results Compared with control group,the proliferation of coronary artery intima was significantly inhibited in PDTC treated rats(P＜0.05). On the 20th day after transplantation,the severity of mononuclear cell infiltration of grafts in PDTC group was markedly decreased(P＜0.05). Conclusion PDTC can suppress the progress of CTV in rat heterotopic transplanted heart.
