CAJ | 학술논문

目的探讨生存素、Ki67在皮肤结外鼻型NK/T细胞淋巴瘤中的表达及意义.方法选取确诊的以皮损为首发表现的15例皮肤结外鼻型NK/T细胞淋巴瘤为研究对象,常规SP免疫组化染色法检测生存素、Ki67.每张切片选取5个有代表性的高倍镜视野,每个视野随机计数200个肿瘤细胞,分别计算1000个肿瘤细胞内生存素、Ki67阳性细胞百分比.结果 15例皮肤结外鼻型NK/T细胞淋巴瘤中,9例瘤细胞表达CD56,13例表达CD3ε,15例表达TIA-1,10例表达粒酶B,2例表达CD3.15例均表达1～2个T细胞抗原(CD2、CD45RO或CD7),1例表达βF1,3例表达CD30的病例阳性细胞均为大瘤细胞.所有病例均不表达CD4、CD5、CD8、CD20和CD79α.14例中3例被检出TCR-γ基因克隆性重排,15例EBER1/2原位杂交均阳性.15例中,11例(73.3％)表达生存素,阳性细胞表达率为23.97％±18.35％；14例(15例中1例掉片)均表达Ki67,阳性细胞表达率41.20％±19.52％.核分裂0～2个/高倍视野的9例Ki67表达阳性细胞率为25.27％±12.96％,核分裂＞2个/高倍视野的6例为58.23％±16.02％,两组差异有统计学意义(F=19.14,P=0.001).皮肤结外鼻型NK/T细胞淋巴瘤中生存素、Ki67都有较高的表达,生存素与Ki67表达之间无相关性.结论生存素、Ki67的高表达可能在皮肤结外鼻型NK/T细胞淋巴瘤发生发展中起着一定作用.
목적 탐토생존소、Ki67재피부결외비형NK/T세포림파류중적표체급의의.방법 선취학진적이피손위수발표현적15례피부결외비형NK/T세포림파류위연구대상,상규SP면역조화염색법검측생존소、Ki67.매장절편선취5개유대표성적고배경시야,매개시야수궤계수200개종류세포,분별계산1000개종류세포내생존소、Ki67양성세포백분비.결과 15례피부결외비형NK/T세포림파류중,9례류세포표체CD56,13례표체CD3ε,15례표체TIA-1,10례표체립매B,2례표체CD3.15례균표체1～2개T세포항원(CD2、CD45RO혹CD7),1례표체βF1,3례표체CD30적병례양성세포균위대류세포.소유병례균불표체CD4、CD5、CD8、CD20화CD79α.14례중3례피검출TCR-γ기인극륭성중배,15례EBER1/2원위잡교균양성.15례중,11례(73.3％)표체생존소,양성세포표체솔위23.97％±18.35％；14례(15례중1례도편)균표체Ki67,양성세포표체솔41.20％±19.52％.핵분렬0～2개/고배시야적9례Ki67표체양성세포솔위25.27％±12.96％,핵분렬＞2개/고배시야적6례위58.23％±16.02％,량조차이유통계학의의(F=19.14,P=0.001).피부결외비형NK/T세포림파류중생존소、Ki67도유교고적표체,생존소여Ki67표체지간무상관성.결론 생존소、Ki67적고표체가능재피부결외비형NK/T세포림파류발생발전중기착일정작용.
Objective To investigate the expressions of survivin and Ki67 in cutaneous extranodal NK/T-cell lymphoma,nasal type and their significance.Methods Clinical data and laboratory test results were collected from 15 cases of cutaneous extranodal NK/T-cell lymphoma,nasal type with skin lesions as the initial manifestation.Immunohistochemical SP method was used to measure the expression of survivin and Ki67 in tissue sections from the lesions of these patients.To determine the percentage of survivin-or Ki67-positive cells,200 tumor cells were counted in a high power field (HPF) and 5 HPFs were observed in 1 section.Results There were 10 males and 5 females among the 15 patients,with the median age at diagnosis being 28.7 (range:9-62) years.Immunohistochemical study showed that the lesional tissue was positive for CD56 in 9 cases,CD3ε in 13 cases,T cell intracellular antigen (TIA)-1 in 15 cases,granzyme B in 10 cases,CD3 in 2 cases,βF1 in 1 case,but negative for CD4,CD5,CD8,CD20,and CD79α.All the 15 cases were positive for 1 or 2 T-cell associated antigens (CD2,CD7 or CD45RO).CD30 was observed in large tumor cells in 3 of the 15 cases.Monoclonal TCR-γ gene rearrangement was found in 3 of 14 cases.In situ hybridization for EBER1/2 was positive in all of the 15 cases.Survivin and Ki67 were expressed in 73.3％ (11/15) and 100％ (14/14,note:a tissue section was unavailable because of the detachment of tissue from the slide) of the specimens respectively,with the average percentage of positive cells being 23.97％ ± 18.35％ and 41.20％ ± 19.52％ for survivin and Ki67,respectively.Statistical differences were observed in the percentage of Ki67-expressing cells between the specimens with 0-2 mitotic figures per high power field from 9 cases and those with more than 2 mitotic figures from 6 cases (25.27％ ± 12.96％ vs.58.23％ ± 16.02％,F =19.14,P =0.001).No correlation was found between the expression of survivin and Ki67 in cutaneous extranodal NK/T-cell lymphoma,nasal type.Conclusion The high expressions of survivin and Ki67 may play a role in the occurrence and development of cutaneous extranodal NK/T-cell lymphoma,nasal type.