国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2010年
5期
272-276
,共5页
胃癌%缺氧诱导因子-1α%血管内皮生长因子-C%淋巴结转移%免疫组织化学
胃癌%缺氧誘導因子-1α%血管內皮生長因子-C%淋巴結轉移%免疫組織化學
위암%결양유도인자-1α%혈관내피생장인자-C%림파결전이%면역조직화학
Gastric carcinoma%HIF-1α%VEGF-C%Lymph node metastasis%Immunohistochemistry
目的 探讨胃癌中缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子-C(VEGF-C)在胃癌中的表达及其临床意义.方法 应用SP免疫组织化学技术检测44例胃癌和20例胃正常组织标本中HIF-1α和VEGF-C的表达,并分析其与胃癌临床病理学特征的关系.结果 HIF-1α在胃癌中的阳性表达率为59.09%(26/44),正常胃黏膜组织中的阳性表达率为25.00%(5/20),两者比较差异有统计学意义(x2=6.59,P＜0.05);其中淋巴结转移阳性组的阳性表达率为72.41%(21/29),淋巴结转移阴性组的阳性表达率为33.33%(5/15)(x2=6.25,P＜0.05),提示HIF-1α与胃癌转移相关.VEGF-C在胃癌中的阳性表达率为52.27%(23/44),正常胃黏膜组织中的阳性表达率为20.00%(4/20),两者比较差异显著(x2=4.73,P＜0.05);其中淋巴结转移阳性组的阳性表达率为65.52%(19/29),淋巴结转移阴性组的阳性表达率为26.67%(4/15),两者比较有统计学意义(x2=4.53,P＜0.05).胃癌中HIF-1α和VEGF-C的表达与肿瘤淋巴结转移和浸润深度有关,而与肿瘤部位、患者性别、年龄无关.HIF-1α与VEGF-C在胃癌组织中的表达呈正相关.结论 胃癌中HIF-1α和VEGF-C的表达上调且二者的表达均与淋巴结转移相关,有望成为胃癌治疗及判断预后的重要指标.
目的 探討胃癌中缺氧誘導因子-1α(HIF-1α)和血管內皮生長因子-C(VEGF-C)在胃癌中的錶達及其臨床意義.方法 應用SP免疫組織化學技術檢測44例胃癌和20例胃正常組織標本中HIF-1α和VEGF-C的錶達,併分析其與胃癌臨床病理學特徵的關繫.結果 HIF-1α在胃癌中的暘性錶達率為59.09%(26/44),正常胃黏膜組織中的暘性錶達率為25.00%(5/20),兩者比較差異有統計學意義(x2=6.59,P＜0.05);其中淋巴結轉移暘性組的暘性錶達率為72.41%(21/29),淋巴結轉移陰性組的暘性錶達率為33.33%(5/15)(x2=6.25,P＜0.05),提示HIF-1α與胃癌轉移相關.VEGF-C在胃癌中的暘性錶達率為52.27%(23/44),正常胃黏膜組織中的暘性錶達率為20.00%(4/20),兩者比較差異顯著(x2=4.73,P＜0.05);其中淋巴結轉移暘性組的暘性錶達率為65.52%(19/29),淋巴結轉移陰性組的暘性錶達率為26.67%(4/15),兩者比較有統計學意義(x2=4.53,P＜0.05).胃癌中HIF-1α和VEGF-C的錶達與腫瘤淋巴結轉移和浸潤深度有關,而與腫瘤部位、患者性彆、年齡無關.HIF-1α與VEGF-C在胃癌組織中的錶達呈正相關.結論 胃癌中HIF-1α和VEGF-C的錶達上調且二者的錶達均與淋巴結轉移相關,有望成為胃癌治療及判斷預後的重要指標.
목적 탐토위암중결양유도인자-1α(HIF-1α)화혈관내피생장인자-C(VEGF-C)재위암중적표체급기림상의의.방법 응용SP면역조직화학기술검측44례위암화20례위정상조직표본중HIF-1α화VEGF-C적표체,병분석기여위암림상병이학특정적관계.결과 HIF-1α재위암중적양성표체솔위59.09%(26/44),정상위점막조직중적양성표체솔위25.00%(5/20),량자비교차이유통계학의의(x2=6.59,P＜0.05);기중림파결전이양성조적양성표체솔위72.41%(21/29),림파결전이음성조적양성표체솔위33.33%(5/15)(x2=6.25,P＜0.05),제시HIF-1α여위암전이상관.VEGF-C재위암중적양성표체솔위52.27%(23/44),정상위점막조직중적양성표체솔위20.00%(4/20),량자비교차이현저(x2=4.73,P＜0.05);기중림파결전이양성조적양성표체솔위65.52%(19/29),림파결전이음성조적양성표체솔위26.67%(4/15),량자비교유통계학의의(x2=4.53,P＜0.05).위암중HIF-1α화VEGF-C적표체여종류림파결전이화침윤심도유관,이여종류부위、환자성별、년령무관.HIF-1α여VEGF-C재위암조직중적표체정정상관.결론 위암중HIF-1α화VEGF-C적표체상조차이자적표체균여림파결전이상관,유망성위위암치료급판단예후적중요지표.
Objective The present studies aimed at studying the expression and significance of HIF-1α and VEGF-C in gastric carcinoma. Methods SP method was used for the immunohistochemical detection of HIF-1α and VEGF-C proteins in paraffin embedded archival samples from 44 patients with gastric carcinoma and 20 normal gastric tissues. Results In 44 cases, the positive rate of HIF-1αwas 59.09%(26/44)and 23 cases 52. 27%(23/44)showed VEGF-C positive in cytoplasm and they were significantly higher than those in gastric normal tissue. The positive rate of HIF-1α in gastric cancer tissue expression with lymph node metastasis was higher than that without lymph node metastasis(72. 41% 21/29 vs 33.33 % 5/15;x2 = 6.25, P ＜ 0. 05).The positive rate of VEGF-C expression with lymph node metastasis and that without lymph node metastasis was 65. 52%(19/29)and 26. 67%(4/15)respectively(x2 = 4. 53, P ＜ 0. 05). The expression of HIF-1α and VEGF-C was closely correlated with lymph node metastasis and the depth of tumor invasion(x2 = 5. 14,P ＜ 0. 05, and x2 = 4. 38, P ＜ 0. 05), but no relationship with tumor position, sex and age. The positive expression of HIF-1α was consistent with the positive expression of VEGF-C. Conclusion The up regulated expression of HIF-1α and VEGF-C were observed in gastric cancer. The expression of HIF-1α and VEGF-C were correlated with lymph node metastasis and they were of some value for predicting prognosis of gastric carcinoma.
