中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2009年
3期
441-442
,共2页
肾%损伤%诊断%实验室技术和方法
腎%損傷%診斷%實驗室技術和方法
신%손상%진단%실험실기술화방법
Kidney%Injuries%Diagnosis%laboratory techniques and procedures
目的 探讨早期诊断肾脏损害的方法.方法 采用酶联免疫吸附法检测尿微量白蛋白(Alb)、尿蛋白1(UPI)、尿视黄醇结合蛋白(RBP),比色法测定N-乙酰-B-D-葡萄苷酶(NAG),酶法测定尿肌酐(Cr).结果 对照组尿Alb/Cr(1.93±0.42)mg/mmol、UPI/Cr(47.61±5.86)μg/mmol、RBP/Cr(24.4±13.55)μg/mmol,NAG/Cr(0.96±0.58)u/mmol,尿蛋白定性阴性的糖尿病、高血压、系统性红斑狼疮及尿路感染患者尿Alb/Cr、UPl/Cr、RBP/Cr及NAG/Cr较对照组显著增高(P＜0.01),单项或双项检测Alb、UPl、RBP或NAG这四项指标阳性率偏低,将这四项指标联合检测在糖尿病、高血压、系统性红斑狼疮及尿路感染中阳性率分别达92%、80%、85%、76%,明显提高了阳性检出率.结论 联合检测尿Alb、UPI、RBP及NAG是诊断早期肾脏损害的灵敏、可靠的实验室指标.
目的 探討早期診斷腎髒損害的方法.方法 採用酶聯免疫吸附法檢測尿微量白蛋白(Alb)、尿蛋白1(UPI)、尿視黃醇結閤蛋白(RBP),比色法測定N-乙酰-B-D-葡萄苷酶(NAG),酶法測定尿肌酐(Cr).結果 對照組尿Alb/Cr(1.93±0.42)mg/mmol、UPI/Cr(47.61±5.86)μg/mmol、RBP/Cr(24.4±13.55)μg/mmol,NAG/Cr(0.96±0.58)u/mmol,尿蛋白定性陰性的糖尿病、高血壓、繫統性紅斑狼瘡及尿路感染患者尿Alb/Cr、UPl/Cr、RBP/Cr及NAG/Cr較對照組顯著增高(P＜0.01),單項或雙項檢測Alb、UPl、RBP或NAG這四項指標暘性率偏低,將這四項指標聯閤檢測在糖尿病、高血壓、繫統性紅斑狼瘡及尿路感染中暘性率分彆達92%、80%、85%、76%,明顯提高瞭暘性檢齣率.結論 聯閤檢測尿Alb、UPI、RBP及NAG是診斷早期腎髒損害的靈敏、可靠的實驗室指標.
목적 탐토조기진단신장손해적방법.방법 채용매련면역흡부법검측뇨미량백단백(Alb)、뇨단백1(UPI)、뇨시황순결합단백(RBP),비색법측정N-을선-B-D-포도감매(NAG),매법측정뇨기항(Cr).결과 대조조뇨Alb/Cr(1.93±0.42)mg/mmol、UPI/Cr(47.61±5.86)μg/mmol、RBP/Cr(24.4±13.55)μg/mmol,NAG/Cr(0.96±0.58)u/mmol,뇨단백정성음성적당뇨병、고혈압、계통성홍반랑창급뇨로감염환자뇨Alb/Cr、UPl/Cr、RBP/Cr급NAG/Cr교대조조현저증고(P＜0.01),단항혹쌍항검측Alb、UPl、RBP혹NAG저사항지표양성솔편저,장저사항지표연합검측재당뇨병、고혈압、계통성홍반랑창급뇨로감염중양성솔분별체92%、80%、85%、76%,명현제고료양성검출솔.결론 연합검측뇨Alb、UPI、RBP급NAG시진단조기신장손해적령민、가고적실험실지표.
Objective To explore diagnostic basis for early renal lnjuryo Methods Enzyme-linked immunosorbent assay wag used,urine protein icroalbumin,Micoalbuminl,retinol binding protein.Urinary N-acetyle B-D-glucosaminidase was determineby chromatometry.Urinary creatinine was determined with enzymic method.Results The levels of urinary transferrin,miroalbumin and NAG in the controls were Alb/Cr(1.93±0.42(mg/mmol),UPI/Cr 47.61±5.86(mg/mmol),RBP/cr:24.41±3.55(mg/mmol),NAG/Cr.0.96±0.58(u/mmol).The levels of urirary transferrin,microalbumin and NAG in urinary albumin negative groups(diabetes,hypertension,lupus erythematosus,Wethra infection)were higher than those in the controls(P＜0.01),positive rates of determining one or two of the four items were lowerthan that of determining Alb UPl RBP and NAG.together.Determing for items together would raise the positive rate to 92% in diabetes,80%in hypertension,85%in lupus erytematosus,76%in urethra infection respectively.Conclusion It is sensitive and reliable tO detect Alb,UPI,RBP and NAG together for diagnosis of early renal injury.
