CAJ | 학술논문

目的观察兔重度眼球穿孔伤视网膜光感受细胞胀亡(oncosis)情况和核糖核酸(RNA)的表达,以及两者在重度眼球损伤中的作用.方法健康新西兰白兔制作重度眼球穿孔伤模型,用甲基绿-派罗宁-马休黄(MG-P-MY)染色法和超高倍镜分别观察细胞胀亡情况及进行细胞RNA检测.结果对照组和1 h组未见胀亡细胞,3 h组出现胀亡细胞,6 h组胀亡细胞数达高峰,12 h组有所下降.3 h组在内核层及神经节细胞层有少量RNA阳性表达,6 h内核层阳性表达增多,12 h阳性表达明显,24 h可见大量RNA阳性细胞.结论眼球枪弹穿孔伤后,胀亡是视网膜细胞死亡的另一种主要方式.在视网膜细胞死亡过程中,胀亡和凋亡是其主要死亡方式.
목적 관찰토중도안구천공상시망막광감수세포창망(oncosis)정황화핵당핵산(RNA)적표체,이급량자재중도안구손상중적작용.방법 건강신서란백토제작중도안구천공상모형,용갑기록-파라저-마휴황(MG-P-MY)염색법화초고배경분별관찰세포창망정황급진행세포RNA검측.결과 대조조화1 h조미견창망세포,3 h조출현창망세포,6 h조창망세포수체고봉,12 h조유소하강.3 h조재내핵층급신경절세포층유소량RNA양성표체,6 h내핵층양성표체증다,12 h양성표체명현,24 h가견대량RNA양성세포.결론 안구창탄천공상후,창망시시망막세포사망적령일충주요방식.재시망막세포사망과정중,창망화조망시기주요사망방식.
Objective To investigate the oncosis ceils and the RNA positive retinal photoreceptor cells after severe penetrating rabbit eye injury.Methods White New Zealand rabbits were selected.The RNA positive cell were observed through MG-P-MY stain methods,and the oncosis cell were observed under extremely high times microscopy.Results There was no oncosis cell in the control group or 1 hour experimental group.The oncosis cells could be seen in 3 hour group,more in 6 hour group,and the oncosis cell number decreased in 12 hour group.In 3 hour group,a lower positive expression of RNA were observed in the ganglion and inner layer cells,it increased obviously in 6 hour group,and there were more RNA expression in 12 hour group.Numerous of RNA expression cells could be seen in 24 hour group.Conclusion The oncosis is the main way of the retinal cell dying after the rabbit penetrating eye injury.In the process of retinal cell death,oncosis and apoptosis is the main mode.