CAJ | 학술논문

研究去核山羊(Capra hircus)体内成熟的MⅡ期卵母细胞与异种成年的哺乳动物(包括山羊、渡尔山羊、牛、塔尔羊、熊猫)及人的成纤维细胞融合形成的体细胞核移植胚胎着床前的发育能力.结果显示这些异种体细胞核移植重构胚可以完成着床前发育,并形成囊胚.种内体细胞核移植胚的融合率和囊胚发育率分别为78.67%(557/708)和56.29%(264/469);亚种问或种问体细胞核移植胚的融合率和囊胚发育率分别为:波尔山羊78.18%(541/692)、33.90%(40/118),牛70.53%(146/207).22.52%(25/111),塔尔羊53.51%(61/114)、5.26%(3/570),熊猫79.82%(1159/1452)、8.35%(75/898),人68.76%(317/461)、5.41%(16/296).由此结果得出以下结论:(1)山羊M Ⅱ期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率;(2)山羊M Ⅱ期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育;(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的.
연구거핵산양(Capra hircus)체내성숙적MⅡ기란모세포여이충성년적포유동물(포괄산양、도이산양、우、탑이양、웅묘)급인적성섬유세포융합형성적체세포핵이식배태착상전적발육능력.결과현시저사이충체세포핵이식중구배가이완성착상전발육,병형성낭배.충내체세포핵이식배적융합솔화낭배발육솔분별위78.67%(557/708)화56.29%(264/469);아충문혹충문체세포핵이식배적융합솔화낭배발육솔분별위:파이산양78.18%(541/692)、33.90%(40/118),우70.53%(146/207).22.52%(25/111),탑이양53.51%(61/114)、5.26%(3/570),웅묘79.82%(1159/1452)、8.35%(75/898),인68.76%(317/461)、5.41%(16/296).유차결과득출이하결론:(1)산양M Ⅱ기란모세포포질여공핵세포지간적친연성불영향량자적융합솔;(2)산양M Ⅱ기란모세포적포질능지지이충간체세포핵이식배적착상전발육;(3)친연관계근적충간핵이식배적낭배발육솔고우친연관계원적충간핵이식배적.
The preimplantation development competences of somatic cell nuclear transfer (SCNT) embryos reconstructed with enuleated goat (Capra hircus) Metaphase Ⅱ (MⅡ) oocytes matured in vivo and whole cells derived from adult fibroblastsof several mammalian species (goat, boer goat, bovine, tahr, panda) and human patient were evaluated. Results obtained from our experiments revealed that these reconstructed SCNT embryos could complete preimplantation development to formblastocysts. The fusion rate and blastocyst rate of intra-species SCNT embryos (Capra hircus as control) was 78.67 (557/708); 56.29% (264/469), that of sub-species or inter-species SCNT embryos were: boer goat 78.18% (541/692); 33.90% (40/118),bovine 70.53% (146/207); 22.52% (25/111), tahr 53.51% (61/114); 5.26% (3/570), panda 79.82% (1159/1452); 8.35% (75/898) and human 68.76% (317/461); 5.41% (16/296), respectively. It is concluded that (1) there are no relationships betweenfusion rate and relativeness of the recipient cytoplasm to nucleus donor cells, (2) cytoplast of the goat MⅡ oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species, (3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.