CAJ | 학술논문

目的探讨嗜酸性粒细胞趋化因子(Eotaxin)、T淋巴细胞激活上调性表达分泌因子(RANTES)在正常妊娠免疫耐受及反复性自然流产中的作用.方法构建正常妊娠小鼠模型CBA/J×BALB/c(正常妊娠组)和反复性自然流产小鼠模型CBA/J×DBA/2J(自然流产组);采用免疫组织化学方法检测胎盘细胞趋化因子Eotaxin、RANTES的表达;采用酶联免疫吸附试验(ELlSA)检测胎盘细胞培养上清液的白细胞介素(IL)-4,干扰素(IFN)-γ水平,分析胎盘细胞趋化因子Eotaxin、RANTES与细胞因子IL-4、IFN-γ水平的相关性.结果自然流产组的胚胎丢失率为18.3%,显著高于正常妊娠组的3.6%(P<0.05).正常妊娠组的胎盘细胞Eotaxin的表达阳性率为53.6%.显著高于自然流产组的40.8%(P(0.05);而正常妊娠组的胎盘细胞RANTES的表达阳性率为44.3%,显著低于自然流产组的62.3%(P<0.05).正常妊娠组的IL-4水平为(1.33±0.43)ng/L,显著高于自然流产组的(0.78±0.41)ng/L(P=0.002);正常妊娠组的IFN-γ水平为(1.24±0.57)ng/L,显著低于自然流产组的(1.67±0.44)ng/L(P=0.038).自然流产组中,胎盘细胞Eotaxin的表达与IL-4水平呈正相关(r=0.752,P=0.003),RANTES的表达与]FN-γ水平亦呈正相关(r=0.658,P=0.014);正常妊娠组中,RANTES的表达与IFN-γ水平呈正相关(r=0.649,P=0.012),IL-4水平随Eotaxin表达阳性率的上升而显示出增加趋势.结论 Eotaxin、RANTES在妊娠免疫耐受形成和自然流产的发病机制中可能发挥重要作用.
목적 탐토기산성립세포추화인자(Eotaxin)、T림파세포격활상조성표체분비인자(RANTES)재정상임신면역내수급반복성자연유산중적작용.방법 구건정상임신소서모형CBA/J×BALB/c(정상임신조)화반복성자연유산소서모형CBA/J×DBA/2J(자연유산조);채용면역조직화학방법검측태반세포추화인자Eotaxin、RANTES적표체;채용매련면역흡부시험(ELlSA)검측태반세포배양상청액적백세포개소(IL)-4,간우소(IFN)-γ수평,분석태반세포추화인자Eotaxin、RANTES여세포인자IL-4、IFN-γ수평적상관성.결과 자연유산조적배태주실솔위18.3%,현저고우정상임신조적3.6%(P<0.05).정상임신조적태반세포Eotaxin적표체양성솔위53.6%.현저고우자연유산조적40.8%(P(0.05);이정상임신조적태반세포RANTES적표체양성솔위44.3%,현저저우자연유산조적62.3%(P<0.05).정상임신조적IL-4수평위(1.33±0.43)ng/L,현저고우자연유산조적(0.78±0.41)ng/L(P=0.002);정상임신조적IFN-γ수평위(1.24±0.57)ng/L,현저저우자연유산조적(1.67±0.44)ng/L(P=0.038).자연유산조중,태반세포Eotaxin적표체여IL-4수평정정상관(r=0.752,P=0.003),RANTES적표체여]FN-γ수평역정정상관(r=0.658,P=0.014);정상임신조중,RANTES적표체여IFN-γ수평정정상관(r=0.649,P=0.012),IL-4수평수Eotaxin표체양성솔적상승이현시출증가추세.결론 Eotaxin、RANTES재임신면역내수형성화자연유산적발병궤제중가능발휘중요작용.
Objective To investigate the role of chemokine condensed from eosinophil chemotaxin (Eotaxin), reduced upon activation, normal T cell expressed and secreted (RANTES) expression in the placenta cells in normal pregnant immune tolerance and repeated spontaneous abortion. Methods Normal pregnant mouse model CBA/J × BALB/C (NNP group) and spontaneous abortion mouse model CBA/J × DBA/2J model (SA group) were established. The expression of Eotaxin, RANTES on cells of the placenta was detected by immunohistochemistry method; the concentrations of interleukin (IL)-4, interferon (IFN)-γ in culture supernatant were determined by ELISA; and the correlation of the chemokine expression with the concentration of cytokine was analyzed. Results The embryo loss rate of SA group (18.3%) was significantly higher compared with that of the NNP group (3.6 %, P<0. 05). The positive rate of Eotaxin in the NNP group (53.6%) was significantly higher than that in the SA group (40.8%, P<0. 05), and the positive rate of RANTES in the NNP group (44.3%) was significantly lower than that in the SA group (62.3 %, P<0. 05). The concentration of IFN-γ ([1.67±0.44] ng/L) in the SA group was significantly higher and the concentration of IL-4 ([0.78 ± 0.41] ng/L) was significantly lower than those in the NNP group (vs. [1.24 ±0.57] ng/L and [1.33 ± 0.43] ng/L, respectively, all P<0.05). We also found that in the SA group the positive rate of Eotaxin was positively correlated with the concentration of IL-4, and the positive rate of RANTES was positively correlated with the concentration of IFN-γ (r = 0. 752, 0. 658, P = 0. 003, 0. 014). In the NNP group the positive rate of RANTES was positively correlated with the concentration of IFN-γ(r= 0. 649, P = 0. 012). IL-4 level increased with the positive rate of Eotaxin. Conclusion Eotaxin and RANTES may play an important role in the pathogenesis of pregnancy immune tolerance and spontaneous abortion.