CAJ | 학술논문

采用蔗糖密度梯度离心法对钝顶螺旋藻光合膜蛋白(PSI)进行分离纯化,并对其光谱学性质、热稳定性及光合放氧活性进行分析表征.结果发现,采用蔗糖密度精度离心法,可以成功分离出4条色素蛋白质复合体条带,其中最下层条带为完整的PSI三聚体,其每毫克叶绿素a光合放氧活性达到420 μmol/h.当温度达到50 ℃左右时,分离得到的PSI在溶液开始变性失活.
채용자당밀도제도리심법대둔정라선조광합막단백(PSI)진행분리순화,병대기광보학성질、열은정성급광합방양활성진행분석표정.결과발현,채용자당밀도정도리심법,가이성공분리출4조색소단백질복합체조대,기중최하층조대위완정적PSI삼취체,기매호극협록소a광합방양활성체도420 μmol/h.당온도체도50 ℃좌우시,분리득도적PSI재용액개시변성실활.
Photosystem I(PSI) of Spirulina platensis was isolated and purified with sucrose density gradient centrifugation using Triton X-100 as a solubilizing agent. The solubilizing ratio of detergent concentration to chlorophyll a of thylakoid membrane was optimized and four pigment bands were obtained. Each band was analyzed by the absorption spectrum, the fluorescence spectrum, and SDS-PAGE. The intact functional PSI trimer was identified as the bottom band with oxygen consumption reached 420 μmol/(mg·h). Circular dichroism spectrum was used to characterize the stability of PSI with the heat treatment.