中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
15期
2671-2674
,共4页
下颌骨%牵引成骨%基因重组人骨形态发生蛋白2%骨组织工程
下頜骨%牽引成骨%基因重組人骨形態髮生蛋白2%骨組織工程
하합골%견인성골%기인중조인골형태발생단백2%골조직공정
背景:重组人骨形态发生蛋白2能诱导未分化的间充质细胞不可逆地分化形成软骨和骨,从而导致新骨的形成.目的:观察在下颌骨牵引成骨过程中应用基因重组人骨形态发生蛋白2(rhBMP-2)对成骨的影响.方法:选用日本成年大耳白兔45只,随机分为3组,建立下颌骨牵引模型,分别将空白胶原、1.5mg和3.0mg重组人骨形态发生蛋白2胶原复合物植入下颌骨切开处,固定、牵引0.4 mm/次,2次/d,共计5d,总延长下颌骨4mm.在稳定期第1,3,7,14,28天分别处死各组动物,取牵引区新生骨痂行放射学及组织学检测.结果与结论:重组人骨形态发生蛋白2组中的新生骨组织较空白胶原对照组中多且成熟,3.0mg重组人骨形态发生蛋白2组较1.5 mg重组人骨形态发生蛋白2组新骨形成多且成熟.结果表明,重组人骨形态发生蛋白2能促进兔下颌骨牵引成骨,并且具有浓度依赖性.
揹景:重組人骨形態髮生蛋白2能誘導未分化的間充質細胞不可逆地分化形成軟骨和骨,從而導緻新骨的形成.目的:觀察在下頜骨牽引成骨過程中應用基因重組人骨形態髮生蛋白2(rhBMP-2)對成骨的影響.方法:選用日本成年大耳白兔45隻,隨機分為3組,建立下頜骨牽引模型,分彆將空白膠原、1.5mg和3.0mg重組人骨形態髮生蛋白2膠原複閤物植入下頜骨切開處,固定、牽引0.4 mm/次,2次/d,共計5d,總延長下頜骨4mm.在穩定期第1,3,7,14,28天分彆處死各組動物,取牽引區新生骨痂行放射學及組織學檢測.結果與結論:重組人骨形態髮生蛋白2組中的新生骨組織較空白膠原對照組中多且成熟,3.0mg重組人骨形態髮生蛋白2組較1.5 mg重組人骨形態髮生蛋白2組新骨形成多且成熟.結果錶明,重組人骨形態髮生蛋白2能促進兔下頜骨牽引成骨,併且具有濃度依賴性.
배경:중조인골형태발생단백2능유도미분화적간충질세포불가역지분화형성연골화골,종이도치신골적형성.목적:관찰재하합골견인성골과정중응용기인중조인골형태발생단백2(rhBMP-2)대성골적영향.방법:선용일본성년대이백토45지,수궤분위3조,건립하합골견인모형,분별장공백효원、1.5mg화3.0mg중조인골형태발생단백2효원복합물식입하합골절개처,고정、견인0.4 mm/차,2차/d,공계5d,총연장하합골4mm.재은정기제1,3,7,14,28천분별처사각조동물,취견인구신생골가행방사학급조직학검측.결과여결론:중조인골형태발생단백2조중적신생골조직교공백효원대조조중다차성숙,3.0mg중조인골형태발생단백2조교1.5 mg중조인골형태발생단백2조신골형성다차성숙.결과표명,중조인골형태발생단백2능촉진토하합골견인성골,병차구유농도의뢰성.
BACKGROUND:Recombinant human bone morphogenetic protein 2(rhBMP-2)can induce undifferentiated mesenchymal cells irreversibly differentiated into cartilage and bone,thus,lead to the fornlation of new bone.OBJECTIVE:To study the effect of rhBMP-2 on the mandibular distraction.METHODS:A totaf of 45 Japanese white rabbits were prepared for mandibular distraction models and randomly assigned into control,1.5 mg rhBMP-2 and 3.0 mg rhBMP-2 groups.Collagen sponge and collagen binding proteins were implanted into mandibles of rabbits,respectively,and distraction 0.4mm per time,twice per day for 5 days.The mandibles were lengthened 4 mm.All rats were sacdficed at 1,3,7,14 and 28 days after operation,and the new generated bone were detected by X-ray and histological examination.RESULTS AND CONCLUSION:Compared with the control group.there were more neonatal bone tissues with maturity in the rhBMP-2 group,especially in the rhBMP-23.0 mg group.The results demonstrated that RhBMP-2 appeam to be able to accelerate bone formation of mandibular distraction osteogenesis in a concentration-dependent manner.
