CAJ | 학술논문

目的:分析临床分离肠外致病大肠杆菌(Extraintestinal pathogenic escherichia coli,ExPEC)的血清型、毒力基因及其与肠内致病大肠杆菌的同源性,探讨ExPEC与肠内致病大肠杆菌的相关性.方法:以我院2006-2009年临床分离的227株ExPEC为研究对象.用常见肠内30种致病大肠杆菌"OK"诊断血清,对上述227株临床分离ExPEC做血清分型,并用PCR分析相关毒力基因,通过脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)分析部分患者ExPEC与该患者肠内大肠杆菌的同源性.结果:在227株ExPEC中,分离出19株肠产毒性大肠杆菌(Enterotoxigenic escherichia coli,ETEC)、15株肠致病性大肠杆菌(Enteropathogenic escherichia coli,EPEC).ETEC不耐热肠毒素基因(Heat-labile enterotoxin,ItA)表达率31.57%、耐热肠毒素基因(Heat-stable enterotoxin,stI)表达率47.36%、stI/ltA21.05%;EPEC紧密粘附素毒力基(E.coli attachment A,eac A)表达率60.0%,束状菌毛毒力基因(Bundle-forming pilus,bfpA)66.66%,eaeA/bfpA 26.66%.PFGE分型:2例患者血培养大肠杆菌与该患者粪便中分离出的大肠杆菌PFGE分型一致.结论:肠内致病大肠杆菌血清型能引起临床肠外感染,但所占比例相对较少,且主要为ETEC和EPEC血清型.ExPEC感染的败血症患者,其来源可能是其肠道致病大肠杆菌的移位所致.
목적:분석림상분리장외치병대장간균(Extraintestinal pathogenic escherichia coli,ExPEC)적혈청형、독력기인급기여장내치병대장간균적동원성,탐토ExPEC여장내치병대장간균적상관성.방법:이아원2006-2009년림상분리적227주ExPEC위연구대상.용상견장내30충치병대장간균"OK"진단혈청,대상술227주림상분리ExPEC주혈청분형,병용PCR분석상관독력기인,통과맥충장응효전영(Pulsed field gel electrophoresis,PFGE)분석부분환자ExPEC여해환자장내대장간균적동원성.결과:재227주ExPEC중,분리출19주장산독성대장간균(Enterotoxigenic escherichia coli,ETEC)、15주장치병성대장간균(Enteropathogenic escherichia coli,EPEC).ETEC불내열장독소기인(Heat-labile enterotoxin,ItA)표체솔31.57%、내열장독소기인(Heat-stable enterotoxin,stI)표체솔47.36%、stI/ltA21.05%;EPEC긴밀점부소독력기(E.coli attachment A,eac A)표체솔60.0%,속상균모독력기인(Bundle-forming pilus,bfpA)66.66%,eaeA/bfpA 26.66%.PFGE분형:2례환자혈배양대장간균여해환자분편중분리출적대장간균PFGE분형일치.결론:장내치병대장간균혈청형능인기림상장외감염,단소점비례상대교소,차주요위ETEC화EPEC혈청형.ExPEC감염적패혈증환자,기래원가능시기장도치병대장간균적이위소치.