CAJ | 학술논문

目的探讨生长抑素类似物奥曲肽能否增强人肺腺癌细胞株A549对化疗药物的敏感性.方法将不同浓度奥曲肽、顺铂和5-氟尿嘧啶(5-Fu)作用于人肺腺癌细胞株A549,运用四甲基偶氮唑盐(MTT)法检测奥曲肽单独以及与顺铂和5-Fu联合作用后48h对A549细胞株生长的影响,每个药物浓度6个复孔,实验重复3次,结果取平均值,并用Isobologram分析评价药物联合应用的作用性质.结果 MTT法结果显示,奥曲肽对A549细胞生长起抑制作用,在浓度1.3～166.7 mg/L范围内,抑制率呈剂量依赖性；其与顺铂和5-Fu共同作用后,对A549细胞的抑制作用明显增高.3种药物联合作用比奥曲肽单用或顺铂、5-Fu两药联合作用差异均具有统计学意义(P＜0.05),其半数抑制浓度(IC50)的效应点落在Isobologram图中的协同区域.结论在体外实验中,奥曲肽能够抑制A549细胞增殖,且联合顺铂和5-Fu后对A549细胞生长抑制作用增强,为协同作用.奥曲肽能提高A549细胞对顺铂和5-Fu的敏感性.
목적 탐토생장억소유사물오곡태능부증강인폐선암세포주A549대화료약물적민감성.방법 장불동농도오곡태、순박화5-불뇨밀정(5-Fu)작용우인폐선암세포주A549,운용사갑기우담서염(MTT)법검측오곡태단독이급여순박화5-Fu연합작용후48h대A549세포주생장적영향,매개약물농도6개복공,실험중복3차,결과취평균치,병용Isobologram분석평개약물연합응용적작용성질.결과 MTT법결과현시,오곡태대A549세포생장기억제작용,재농도1.3～166.7 mg/L범위내,억제솔정제량의뢰성；기여순박화5-Fu공동작용후,대A549세포적억제작용명현증고.3충약물연합작용비오곡태단용혹순박、5-Fu량약연합작용차이균구유통계학의의(P＜0.05),기반수억제농도(IC50)적효응점락재Isobologram도중적협동구역.결론 재체외실험중,오곡태능구억제A549세포증식,차연합순박화5-Fu후대A549세포생장억제작용증강,위협동작용.오곡태능제고A549세포대순박화5-Fu적민감성.
Objective To investigate whether octreotide,as somatostatin analogue,can enhance the sensitivity of the human lung adenocarcinoma cell line A549 to chemotherapeutic drugs.Methods Different concentration of octretide,cisplatin and 5-Fluorouracil (5-Fu) was respectively acted on the lung adenocarcinoma cell line A549.The absorbance value was tested by colorimetry through MTT method to evaluate the effect of octreotide,cisplatin,5-Fu or the three drugs combined respectively after 48 hours.Each drug concentration had six holes and it repeated three times.The effects of combination therapy was analysed with isobologram.Results It was proved that octreotide could inhibit the proliferation of A549 cells in a dose-dependent manner at the concentration range of 1.3 mg/L ～ 166.7 mg/L.The inhibition rate was dose-dependent which was higher when octreotide combined with cisplatin and 5-Fu than it alone.It has statistically significant difference (P ＜ 0.05 ).The effect plots of IC50 were located in the synergy areas of isobologram.Conclusion It can be concluded that octreotide could inhibit the proliferation of A549 cells in vitro.This inhibition enhances when octreotide is combined with cisplatin and 5-Fu.Octreotide can enhance the susceptibility of A549 cells to cisplatin and 5-Fu.