中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
10期
590-594
,共5页
马善奋%梁景平%姜云涛%朱彩莲
馬善奮%樑景平%薑雲濤%硃綵蓮
마선강%량경평%강운도%주채련
牙根龋%牙菌斑%细菌群落%变性梯度凝胶电泳
牙根齲%牙菌斑%細菌群落%變性梯度凝膠電泳
아근우%아균반%세균군락%변성제도응효전영
Root caries%Dental plaque%Bacterial floral%Denaturing gradient gel electrophoresis
目的 研究老年人根面龋牙菌斑内细菌群落构成,以期为今后深入研究根面龋的病因提供依据.方法 提取9例60岁以上根面龋患者9颗患牙的牙菌斑细菌DNA,应用聚合酶链反应-变性梯度凝胶电泳( PCR-denaturing gradient gel electrophoresis,PCR-DGGE)技术扩增,克隆、测序并与核酸序列数据库进行比对,鉴定优势菌属种类.结果 老年人根面龋牙菌斑中共检测到114个细菌基因型,分属于不动杆菌属[0.9％ (1/114)]、放线杆菌属[1.8％ (2/114)]、放线菌属[15.8％( 18/114)]、Aggregatibacter菌属[0.9％(1/114)]、二氧化碳嗜纤维菌属[14.0％ (16/114)]、棒状杆菌属[0.9％ (1/114)]、嗜血杆菌属[0.9％ (1/114)]、动弯杆菌属[0.9％ (1/114)]、纳西杆菌属[0.9％(1/114)]、奈瑟球菌属[10.5％ (12/114)]、卟啉单胞菌属[0.9％ (1/114)]、普氏菌属[12.3％(14/114)]、新月单胞菌属[6.1％ (7/114)]、葡萄球菌属[1.8％ (2/114)]、口腔链球菌群[6.1％(7/114)]和变异链球菌群[7.9％ (9/114)]、Tannerella菌属[0.9％ (1/114)]、密螺旋体[1.8％(2/114)]和韦永球菌属[10.5％ (12/114)],以及两例未获培养的不知名菌种[1.8％ (2/114)].未获培养的基因型占总数的19.3％(22/114).革兰阳性细菌的基因型占31.6％(36/114),革兰阴性细菌的基因型占66.7％ (76/114).结论 老年人根面龋牙菌斑中细菌基因型多且分布较广,以革兰阴性菌占多数；未发现特异性致龋细菌基因型.
目的 研究老年人根麵齲牙菌斑內細菌群落構成,以期為今後深入研究根麵齲的病因提供依據.方法 提取9例60歲以上根麵齲患者9顆患牙的牙菌斑細菌DNA,應用聚閤酶鏈反應-變性梯度凝膠電泳( PCR-denaturing gradient gel electrophoresis,PCR-DGGE)技術擴增,剋隆、測序併與覈痠序列數據庫進行比對,鑒定優勢菌屬種類.結果 老年人根麵齲牙菌斑中共檢測到114箇細菌基因型,分屬于不動桿菌屬[0.9％ (1/114)]、放線桿菌屬[1.8％ (2/114)]、放線菌屬[15.8％( 18/114)]、Aggregatibacter菌屬[0.9％(1/114)]、二氧化碳嗜纖維菌屬[14.0％ (16/114)]、棒狀桿菌屬[0.9％ (1/114)]、嗜血桿菌屬[0.9％ (1/114)]、動彎桿菌屬[0.9％ (1/114)]、納西桿菌屬[0.9％(1/114)]、奈瑟毬菌屬[10.5％ (12/114)]、卟啉單胞菌屬[0.9％ (1/114)]、普氏菌屬[12.3％(14/114)]、新月單胞菌屬[6.1％ (7/114)]、葡萄毬菌屬[1.8％ (2/114)]、口腔鏈毬菌群[6.1％(7/114)]和變異鏈毬菌群[7.9％ (9/114)]、Tannerella菌屬[0.9％ (1/114)]、密螺鏇體[1.8％(2/114)]和韋永毬菌屬[10.5％ (12/114)],以及兩例未穫培養的不知名菌種[1.8％ (2/114)].未穫培養的基因型佔總數的19.3％(22/114).革蘭暘性細菌的基因型佔31.6％(36/114),革蘭陰性細菌的基因型佔66.7％ (76/114).結論 老年人根麵齲牙菌斑中細菌基因型多且分佈較廣,以革蘭陰性菌佔多數；未髮現特異性緻齲細菌基因型.
목적 연구노년인근면우아균반내세균군락구성,이기위금후심입연구근면우적병인제공의거.방법 제취9례60세이상근면우환자9과환아적아균반세균DNA,응용취합매련반응-변성제도응효전영( PCR-denaturing gradient gel electrophoresis,PCR-DGGE)기술확증,극륭、측서병여핵산서렬수거고진행비대,감정우세균속충류.결과 노년인근면우아균반중공검측도114개세균기인형,분속우불동간균속[0.9％ (1/114)]、방선간균속[1.8％ (2/114)]、방선균속[15.8％( 18/114)]、Aggregatibacter균속[0.9％(1/114)]、이양화탄기섬유균속[14.0％ (16/114)]、봉상간균속[0.9％ (1/114)]、기혈간균속[0.9％ (1/114)]、동만간균속[0.9％ (1/114)]、납서간균속[0.9％(1/114)]、내슬구균속[10.5％ (12/114)]、계람단포균속[0.9％ (1/114)]、보씨균속[12.3％(14/114)]、신월단포균속[6.1％ (7/114)]、포도구균속[1.8％ (2/114)]、구강련구균군[6.1％(7/114)]화변이련구균군[7.9％ (9/114)]、Tannerella균속[0.9％ (1/114)]、밀라선체[1.8％(2/114)]화위영구균속[10.5％ (12/114)],이급량례미획배양적불지명균충[1.8％ (2/114)].미획배양적기인형점총수적19.3％(22/114).혁란양성세균적기인형점31.6％(36/114),혁란음성세균적기인형점66.7％ (76/114).결론 노년인근면우아균반중세균기인형다차분포교엄,이혁란음성균점다수；미발현특이성치우세균기인형.
Objective To analyze the community in dental plaque of elder people with root caries.Methods Total DNAs were extracted from the root caries dental plaques of nine elders over 60 years of age.Polymerase chaid reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial composition,DGGE bands were excised from the gels for sequencing and identification.Results The dominant genus in root caries dental plaque of elder people were:Acinetobacte [0.9％ (1/114) ],Actinobaculum [ 1.8％ ( 2/114 ) ],Actinomyces [ 15.8％ ( 18/114 ) ],Aggregatibacter [0.9％ (1/114) ],Capnocytophaga [ 14.0％ ( 16/114 ) ],Corynebacterium [ 0.9％ ( 1/114 ) ],Haemophilus [0.9％ ( 1/1 14 ) ],Mobiluncus [ 0.9％ ( 1/114 ) ],Naxibacter [ 0.9％ ( 1/1 14 ) ],Neisseriaceae [ 10.5％ (12/114) ],Porphyromonas [ 0.9％ ( 1/114 ) ],Prevotella [ 12.3％ ( 14/114 ) ],Selenomonas [ 6.1％(7/114 ) ],Staphylococcus [ 1.8％ ( 2/114 ) ],Oralis streptococcus [ 6.1％ ( 7/114 ) ],Mutans streptococcu [7.9％ (9/114) ],Tannerella [0.9％ ( 1/114)],Treponema [ 1.8％ (2/114)],Veillonella [ 10.5％(12/114) ] and two uncultured unknown genus [ 1.8％ (2/114 )].Uncultred genotypes accounted for 19.30％ of the total.Gram-positive bacteria genotype accounted for 31.6％ (36/114),and Gram-negative bacteria genotype accounted for 66.7％ (76/114).Conclusions There were many bacteria genotypes in root caries dental plaque in the elderly,which were widely distributed.Gram-negative bacteria accounted for the majority.Genotype-specific pathogenic bacteria were not found.
