国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2009年
5期
394-397
,共4页
赵海涛%温培娥%任霞%杨炜华%范华%乔高娟%唐天华%任海全%毕可红%姜国胜
趙海濤%溫培娥%任霞%楊煒華%範華%喬高娟%唐天華%任海全%畢可紅%薑國勝
조해도%온배아%임하%양위화%범화%교고연%당천화%임해전%필가홍%강국성
细胞凋亡%砷剂
細胞凋亡%砷劑
세포조망%신제
Apoptosis%Arsenicals
目的 研究PML-RARα表达阴性的HL-60细胞和表达阳性的NB4细胞对三氧化二砷(As2O3)诱导细胞凋亡敏感性差异的分子机制.方法 经As2O3作用人急性早幼粒细胞白血病HL-60和NB4细胞后,MTT法检测细胞增殖变化,流式细胞术测定细胞凋亡水平的变化,RT-PCR检测Bcl-2、Bax、Fas等基因在mRNA水平的表达变化.结果 As2O3可诱导NIM细胞出现明显的凋亡现象,细胞增殖受到明显抑制(P<0.05),Bcl-2基因表达明显下调(P<0.05),但Bax、Fas基因表达没有明显变化(P>0.05),Bcl-2/Bax比值明显降低(P<0.05).而对于PML-RARa表达阴性的HL-60细胞,经小剂量As2O3作用后,对细胞的增殖抑制作用不明显(P>0.05),未出现明显凋亡现象,Bcl-2、Bax、Fas或Bcl-2/Bax表达水平及比值变化不明显(P>0.05).结论 Bcl-2/Bax表达比值变化与否,可能是小剂量As2O3诱导PML-RARα表达阴性的HL-60细胞和表达阳性的NB4细胞发生凋亡差异的分子机制.
目的 研究PML-RARα錶達陰性的HL-60細胞和錶達暘性的NB4細胞對三氧化二砷(As2O3)誘導細胞凋亡敏感性差異的分子機製.方法 經As2O3作用人急性早幼粒細胞白血病HL-60和NB4細胞後,MTT法檢測細胞增殖變化,流式細胞術測定細胞凋亡水平的變化,RT-PCR檢測Bcl-2、Bax、Fas等基因在mRNA水平的錶達變化.結果 As2O3可誘導NIM細胞齣現明顯的凋亡現象,細胞增殖受到明顯抑製(P<0.05),Bcl-2基因錶達明顯下調(P<0.05),但Bax、Fas基因錶達沒有明顯變化(P>0.05),Bcl-2/Bax比值明顯降低(P<0.05).而對于PML-RARa錶達陰性的HL-60細胞,經小劑量As2O3作用後,對細胞的增殖抑製作用不明顯(P>0.05),未齣現明顯凋亡現象,Bcl-2、Bax、Fas或Bcl-2/Bax錶達水平及比值變化不明顯(P>0.05).結論 Bcl-2/Bax錶達比值變化與否,可能是小劑量As2O3誘導PML-RARα錶達陰性的HL-60細胞和錶達暘性的NB4細胞髮生凋亡差異的分子機製.
목적 연구PML-RARα표체음성적HL-60세포화표체양성적NB4세포대삼양화이신(As2O3)유도세포조망민감성차이적분자궤제.방법 경As2O3작용인급성조유립세포백혈병HL-60화NB4세포후,MTT법검측세포증식변화,류식세포술측정세포조망수평적변화,RT-PCR검측Bcl-2、Bax、Fas등기인재mRNA수평적표체변화.결과 As2O3가유도NIM세포출현명현적조망현상,세포증식수도명현억제(P<0.05),Bcl-2기인표체명현하조(P<0.05),단Bax、Fas기인표체몰유명현변화(P>0.05),Bcl-2/Bax비치명현강저(P<0.05).이대우PML-RARa표체음성적HL-60세포,경소제량As2O3작용후,대세포적증식억제작용불명현(P>0.05),미출현명현조망현상,Bcl-2、Bax、Fas혹Bcl-2/Bax표체수평급비치변화불명현(P>0.05).결론 Bcl-2/Bax표체비치변화여부,가능시소제량As2O3유도PML-RARα표체음성적HL-60세포화표체양성적NB4세포발생조망차이적분자궤제.
Objective To study the molecular mechanism of different sensitivities to apoptosis induced by low concentration of As2O3 in PML-RARα negative HL-60 cells and PML-RARα positive NB4 cells. Meth-ods NB4 and HL-60 cells were cultured with As2O3 for 1 to 4 days; cell proliferation were detected by MTT method; the apoptosis was detected by flow cytometry,Bcl-2,Bax and Fas mRNA were determined by RT-PCR. Results The proliferation of NB4 cells was inhibited obviously by As2O3(1.0 μmol/L)with the induction of apoptosis( P <0.05) ,which was accompanied by the down-regulation of Bcl-2 mRNA expression( P <0.05)and the ratio of Bcl-2/Bax(P <0.05), but there was no obvious variation of Bax and Fas expression( P >0.05). Inhibition of proliferation and apoptosis were not obvious in PML-RARα negative HL-60 cells induced by low concentration As2O3 ( P >0.05), and there was no obvious variation of Bcl-2, Bax, Fas mRNA expres-sion or Bcl/Bax ratio( P >0.05). Conclusion The ratio of Bcl-2/Bax is contributed to the different sensitiv-ities of PML-RARα negative HL-60 cells and positive NB4 cells induced by low concentration of As2O3.
