CAJ | 학술논문

目的探讨内毒素(LPS)激活X盒结合蛋白1(XBP1)信号转导通路对移植肝缺血再灌注损伤的影响及其机制.方法以雄性SD大鼠为供、受者,分为冷缺血转染组、活体转染组和对照组,以两袖套法建立肝移植模型.冷缺血转染组大鼠于冷缺血期经门静脉灌注转染携带XBP1短发夹干扰RNA的质粒(pSIXBP1);活体转染组大鼠在门静脉袖套吻合完成后经门静脉分支注入pSIXBP1;对照组不予任何处理.分别于移植肝门静脉恢复再灌注后60和180 min处死大鼠,光镜观察肝组织病理损害程度,逆转录聚合酶链反应及蛋白免疫印记法测定肝组织XBP1 mRNA和XBP的表达水平;酶连免疫吸附试验检测肝组织核因子κB(NF-κB)的活性及血清肿瘤坏死因子α(TNF-α)的含量.结果再灌注后180 min,冷缺血转染组病理损害程度、XBP1 mRNA含量和XBP1含量低于活体转染组和对照组(P＜0.05),该组TNF-α的表达水平为(584.94±15.97)pg/ml,低于活体转染组和对照组(P＜0.05).而再灌注后180 min时,3组NF-κB活性的差异无统计学意义(P＞0.05).结论 XBP1通路与NF-κB通路在大鼠肝脏缺血再灌注损伤中对TNF-α基因的调控作用是两个相对独立的环节,XBP1短发夹RNA干扰技术能有效减轻肝移植时的缺血再灌注损伤.
목적 탐토내독소(LPS)격활X합결합단백1(XBP1)신호전도통로대이식간결혈재관주손상적영향급기궤제.방법 이웅성SD대서위공、수자,분위랭결혈전염조、활체전염조화대조조,이량수투법건립간이식모형.랭결혈전염조대서우랭결혈기경문정맥관주전염휴대XBP1단발협간우RNA적질립(pSIXBP1);활체전염조대서재문정맥수투문합완성후경문정맥분지주입pSIXBP1;대조조불여임하처리.분별우이식간문정맥회복재관주후60화180 min처사대서,광경관찰간조직병리손해정도,역전록취합매련반응급단백면역인기법측정간조직XBP1 mRNA화XBP적표체수평;매련면역흡부시험검측간조직핵인자κB(NF-κB)적활성급혈청종류배사인자α(TNF-α)적함량.결과 재관주후180 min,랭결혈전염조병리손해정도、XBP1 mRNA함량화XBP1함량저우활체전염조화대조조(P＜0.05),해조TNF-α적표체수평위(584.94±15.97)pg/ml,저우활체전염조화대조조(P＜0.05).이재관주후180 min시,3조NF-κB활성적차이무통계학의의(P＞0.05).결론 XBP1통로여NF-κB통로재대서간장결혈재관주손상중대TNF-α기인적조공작용시량개상대독립적배절,XBP1단발협RNA간우기술능유효감경간이식시적결혈재관주손상.
Objective To explore the regulation mechanism of X box binding protein 1 (XBP1)signal transduction pathway for TNF-α and effective approach in ischemia/reperfusion (I/R) injury of liver transplantation for short hairpin RNA (shRNA) interference used to gene therapy in liver graft.Methods Male Sprague-Dawley rats were divided into three groups: the cold ischemia transfection group (CIT), the in vivo transfection group (IVT) and the control group. Experiments of orthotopic liver transplantation were performed by two cuff method. The rats in CIT were perfused with XBP1-shRNA plasmid (pSIXBP1) during cold ischemia phase, those in IVT received the equivalent volume (2 ml) of pSIIRAK 4 after portal vein inoculation, and those in the control group were not subjected to any treatment. Rats were killed at 60 or 180 min after restoring reperfusion of hepatic portal vein.Histopathological damage degree of graft liver was observed by light microscope. The expression levels of XBP1 gene and protein were detected by RT-PCR and Western blotting. The activities of NF-κB and the serum TNF-α level were detected by ELISA. Results All the indexes including the degree of histopathological damage, the expression levels of XBP1 mRNA and protein and the TNF-α level were significantly decreased in CIT as compared with IVT and control group (P＜0. 05). However,there was no significant difference in NF-κB activity among the three groups (P＞0. 05). Conclusion The role of XBP1 pathway in TNF-α gene regulation and that of NF-κB pathway in rat liver I/R injury are two relatively independent aspects, and the depression of XBP1 expression with XBP1 shRNA through portal vein perfusion during cold ischemia phase could effectively alleviate graft hepatic I/R