肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
4期
232-235
,共4页
李大林%章婕%傅振坤%张明焱%许凤燕%庞达
李大林%章婕%傅振坤%張明焱%許鳳燕%龐達
리대림%장첩%부진곤%장명염%허봉연%방체
乳腺肿瘤%多态性,单核苷酸%疾病易感性
乳腺腫瘤%多態性,單覈苷痠%疾病易感性
유선종류%다태성,단핵감산%질병역감성
Breast neoplasms%Polymurphism,single nucleotide%Disease susceptibility
目的 探讨B7-H4基因rs10754339、rs10801935和rs3738414等SNP位点多态性与黑龙江省妇女乳腺癌发病风险的相关性.方法 利用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术,对287例黑龙江省汉族女性乳腺癌患者和305名健康对照者进行B7-H4基因位点多态性检测,并确定常见单体型(频率≥1%),分析患者与健康对照差异,进而确定该基因与黑龙江省汉族妇女乳腺癌的相关性.结果 B7-H4基因3'-UTR区rs10754339位点的G等位基因、AA、AG基因型发生频率在乳腺癌组和健康对照组间差异有统计学意义(P=0.030,OR 1.359,95% CI 1.030～1.794;P=0.018,OR 0.671,95%CI 0.482～0.935;P=0.029,OR 1.455,95%CI 1.038～2.038).5'-UTR区rs3738414位点的A等位基因、GG基因型和AG基因型发生频率在乳腺癌患者和健康对照组之间差异有统计学意义(P=0.0008,OR 0.604,95% CI 0.455～0.803;P=0.001,OR 1.804,95% CI 1.289～2.253;P=0.005,OR 0.612,95%CI 0.435～0.862).AAA单体型和GAG单体型发生频率在乳腺癌患者和健康对照组之间差异有统计学意义(P=0.0015,OR 0.614,95% CI 0.456～0.828;P=0.0003,OR 1.954,95% CI 1.363～2.803).结论 B7-H4基因多态性与黑龙江省汉族妇女乳腺癌发病存在一定相关性.
目的 探討B7-H4基因rs10754339、rs10801935和rs3738414等SNP位點多態性與黑龍江省婦女乳腺癌髮病風險的相關性.方法 利用聚閤酶鏈反應限製性片段長度多態性(PCR-RFLP)技術,對287例黑龍江省漢族女性乳腺癌患者和305名健康對照者進行B7-H4基因位點多態性檢測,併確定常見單體型(頻率≥1%),分析患者與健康對照差異,進而確定該基因與黑龍江省漢族婦女乳腺癌的相關性.結果 B7-H4基因3'-UTR區rs10754339位點的G等位基因、AA、AG基因型髮生頻率在乳腺癌組和健康對照組間差異有統計學意義(P=0.030,OR 1.359,95% CI 1.030～1.794;P=0.018,OR 0.671,95%CI 0.482～0.935;P=0.029,OR 1.455,95%CI 1.038～2.038).5'-UTR區rs3738414位點的A等位基因、GG基因型和AG基因型髮生頻率在乳腺癌患者和健康對照組之間差異有統計學意義(P=0.0008,OR 0.604,95% CI 0.455～0.803;P=0.001,OR 1.804,95% CI 1.289～2.253;P=0.005,OR 0.612,95%CI 0.435～0.862).AAA單體型和GAG單體型髮生頻率在乳腺癌患者和健康對照組之間差異有統計學意義(P=0.0015,OR 0.614,95% CI 0.456～0.828;P=0.0003,OR 1.954,95% CI 1.363～2.803).結論 B7-H4基因多態性與黑龍江省漢族婦女乳腺癌髮病存在一定相關性.
목적 탐토B7-H4기인rs10754339、rs10801935화rs3738414등SNP위점다태성여흑룡강성부녀유선암발병풍험적상관성.방법 이용취합매련반응한제성편단장도다태성(PCR-RFLP)기술,대287례흑룡강성한족녀성유선암환자화305명건강대조자진행B7-H4기인위점다태성검측,병학정상견단체형(빈솔≥1%),분석환자여건강대조차이,진이학정해기인여흑룡강성한족부녀유선암적상관성.결과 B7-H4기인3'-UTR구rs10754339위점적G등위기인、AA、AG기인형발생빈솔재유선암조화건강대조조간차이유통계학의의(P=0.030,OR 1.359,95% CI 1.030～1.794;P=0.018,OR 0.671,95%CI 0.482～0.935;P=0.029,OR 1.455,95%CI 1.038～2.038).5'-UTR구rs3738414위점적A등위기인、GG기인형화AG기인형발생빈솔재유선암환자화건강대조조지간차이유통계학의의(P=0.0008,OR 0.604,95% CI 0.455～0.803;P=0.001,OR 1.804,95% CI 1.289～2.253;P=0.005,OR 0.612,95%CI 0.435～0.862).AAA단체형화GAG단체형발생빈솔재유선암환자화건강대조조지간차이유통계학의의(P=0.0015,OR 0.614,95% CI 0.456～0.828;P=0.0003,OR 1.954,95% CI 1.363～2.803).결론 B7-H4기인다태성여흑룡강성한족부녀유선암발병존재일정상관성.
Objective To evaluate the association between the polymorphisms of BT-H4 gene and the risk of breast cancer, a case-control study was conducted in the population of Heilongjiang province, China. Methods We genotyped the single nucleotide polymorphisms of rs10754339, rs10801935 and rs3738414 in B7-H4 gene by PCR-RFLP in a Chinese population consisting of 287 breast cancer cases and 305 controls matched for age and sex, tagged all common haplotypes (frequency ≥ 1%), and analyzed the differences between patients and normal controls. Results Our data indicated that in rs10754339, the frequencies of G allele, AA genotype and AG genotype were significantly different between patients and controls (P=0.030, OR 1.359, 95 % CI 1.030-1.794; P=0.018, OR 0.671, 95 % CI 0.482-0.935; P=0.029, OR 1.455, 95 % CI 1.038-2.038, respectively). In rs3738414, the frequencies of A allele, GG genotype and AG genotype were significantly different between patients and controls (P=0.0008, OR 0.604, 95 % CI 0.455-0.803; P=0.001, OR 1.804, 95 % CI 1.289-2.253; P=0.005, OR 0.612, 95 % CI 0.435-0.862). The frequencies of AAA haplotype and GAG haplotype were significantly different between patients and controls (P=0.0015, OR 0.614, 95 % CI 0.456-0.828; P=0.0003, OR 1.954, 95 % CI 1.363-2.803). Conclusion Polymorphisms of B7-H4 gene appear to be associated with breast cancer in the population of Heilongjiang province, China.
