CAJ | 학술논문

对采自连云港海域的海泥样品进行放线菌选择性分离,用其发酵液进行抗肿瘤活性筛选,并对活性较好的菌株HGF26进行了初步鉴定.从海泥样品中共分离得到放线菌78株,以人肝癌细胞HepG2为靶标,活性筛选得到细胞毒活性达60%以上的阳性菌株3株,以其他5株肿瘤细胞为靶标的复筛表明,菌株HGF26的发酵液对多种肿瘤细胞具有显著的细胞毒活性,其中对胃癌细胞BGC823的细胞毒活性为79%,活性产物具有较好的酸碱和热稳定性.通过对菌株的培养特征、形态特征、生理生化特征和细胞壁成分分析,将菌株HGF26初步鉴定为微白黄链霉菌的海洋变种.
대채자련운항해역적해니양품진행방선균선택성분리,용기발효액진행항종류활성사선,병대활성교호적균주HGF26진행료초보감정.종해니양품중공분리득도방선균78주,이인간암세포HepG2위파표,활성사선득도세포독활성체60%이상적양성균주3주,이기타5주종류세포위파표적복사표명,균주HGF26적발효액대다충종류세포구유현저적세포독활성,기중대위암세포BGC823적세포독활성위79%,활성산물구유교호적산감화열은정성.통과대균주적배양특정、형태특정、생리생화특정화세포벽성분분석,장균주HGF26초보감정위미백황련매균적해양변충.
Actinomycete selective isolation was carried out with marine mud samples collected from sea area in Li-anyungang, a screening for antitumor activity with their fermentation broth, and a better strain in the antitumor activity HGF26 was initially characterized. Altogether 78 strains were isolated from the mud samples, 3 positive of them were screened to have over 60% of cytotoxic activity taking human hepatoma cell HepG2 as a target; it was also indicated by re-screening with another five strains tumor cell that fermentation broth of strain HGF26 had a strong cytoxic activity against many kinds of tumor cell, the cytotoxic activity against gastric carcinoma cell BGC823 was as high as 79% , and the active product having acid-and-alkaline and heat stability, h was characterized that HGF26 as Streptomyces al-bidoflavas var. marinus through culture, morphological, physiological and biochemical features, and cell wall compo-nent analysis.