时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
4期
784-786
,共3页
瞿畅%赖志才%裴赢%张美英%王一飞%张颖君%杨崇仁
瞿暢%賴誌纔%裴贏%張美英%王一飛%張穎君%楊崇仁
구창%뢰지재%배영%장미영%왕일비%장영군%양숭인
余甘子%单纯疱疹病毒1型%单纯疱疹病毒2型%细胞病变效应法%空斑减数法
餘甘子%單純皰疹病毒1型%單純皰疹病毒2型%細胞病變效應法%空斑減數法
여감자%단순포진병독1형%단순포진병독2형%세포병변효응법%공반감수법
Phyllanthus emblica%HSV-1%HSV-2%CPE%Plaques reduction ratio
目的 从余甘子的醋酸乙酯提取物、甲醇提取物和水提物3种提取物体外筛选抗单纯疱疹病毒1型和2型的活性成分.方法 通过观察药物毒性、病毒引起的细胞病变效应、空斑减数法,判断药物的毒性及其抗病毒效应.结果 余甘子的醋酸乙酯提取物、甲醇提取物和水提物对于单纯疱疹病毒1型的半数抑制浓度分别为25.28,66.17,100.94 μg/ml;余甘子的3种提取物对于单纯疱疹病毒2型的半数抑制浓度分别为31.70,180.3,112.1 μg/ml.结论 余甘子3种提取物在体外对单纯疱疹病毒1型和2型均有一定的抑制作用.
目的 從餘甘子的醋痠乙酯提取物、甲醇提取物和水提物3種提取物體外篩選抗單純皰疹病毒1型和2型的活性成分.方法 通過觀察藥物毒性、病毒引起的細胞病變效應、空斑減數法,判斷藥物的毒性及其抗病毒效應.結果 餘甘子的醋痠乙酯提取物、甲醇提取物和水提物對于單純皰疹病毒1型的半數抑製濃度分彆為25.28,66.17,100.94 μg/ml;餘甘子的3種提取物對于單純皰疹病毒2型的半數抑製濃度分彆為31.70,180.3,112.1 μg/ml.結論 餘甘子3種提取物在體外對單純皰疹病毒1型和2型均有一定的抑製作用.
목적 종여감자적작산을지제취물、갑순제취물화수제물3충제취물체외사선항단순포진병독1형화2형적활성성분.방법 통과관찰약물독성、병독인기적세포병변효응、공반감수법,판단약물적독성급기항병독효응.결과 여감자적작산을지제취물、갑순제취물화수제물대우단순포진병독1형적반수억제농도분별위25.28,66.17,100.94 μg/ml;여감자적3충제취물대우단순포진병독2형적반수억제농도분별위31.70,180.3,112.1 μg/ml.결론 여감자3충제취물재체외대단순포진병독1형화2형균유일정적억제작용.
Objective To study the active components of anti- HSV-1 and HSV-2 of crude extract from Phyllanthus emblica.Methods The toxic and antivirous effects were evaluated by observing toxic effect,cytopathic effect(CPE),MTT colorimetric assay for vital cell rate,plaques reduction ratio.Results Inhibition on herpes simplex virus were observed during the experiment.The IC_(50) on HSV-1 of acetic ether extract,methanol extract,aqueous extract from Phyllanthus emblica were 25.28,66.17,100.94μg/ml; while the IC_(50) on HSV-2 of the extracts above were 31.70,180.3,112.1μg/ml.Conclusion The three extracts from Phyllanthus emblica can inhibit both of HSV-1 and HSV-2 in vitro.
