湖北农业科学
湖北農業科學
호북농업과학
2010年
3期
630-631
,共2页
北五味子%组织培养%培养基
北五味子%組織培養%培養基
북오미자%조직배양%배양기
Schisandra sphenanthera Rehd. et Wils%tissue culture%culture medium
以北五味子的茎为外植体,采用3种不同的MS培养基对其进行诱导,探讨不同浓度NAA、2.4-D对北五味子茎的诱导成活率的影响.结果表明,MS+NAA 0.1 mg/L+2,4-D 0.5mg/L+6-BA 2ms/L+蔗糖30g/L+琼脂9g/L培养基对北五味子茎的诱导成活率最高,为最佳诱导分化培养基.
以北五味子的莖為外植體,採用3種不同的MS培養基對其進行誘導,探討不同濃度NAA、2.4-D對北五味子莖的誘導成活率的影響.結果錶明,MS+NAA 0.1 mg/L+2,4-D 0.5mg/L+6-BA 2ms/L+蔗糖30g/L+瓊脂9g/L培養基對北五味子莖的誘導成活率最高,為最佳誘導分化培養基.
이북오미자적경위외식체,채용3충불동적MS배양기대기진행유도,탐토불동농도NAA、2.4-D대북오미자경적유도성활솔적영향.결과표명,MS+NAA 0.1 mg/L+2,4-D 0.5mg/L+6-BA 2ms/L+자당30g/L+경지9g/L배양기대북오미자경적유도성활솔최고,위최가유도분화배양기.
Using four kinds of MS culture medium, the effects of different combinations of NAA and the 2,4-D as A (0.1 mg/L,0.5 mg/L), B (0.3 mg/L,0.3 mg/L), C (0.5 mg/L,0.1 mg/L) and CK(0 mg/L) on induction and differentiation of stem in Schisandra sphenanthera Rehd. et Wils were studied. The findings indicated that the culture medium A (0.1 mg/L,0.5 mg/L) was the best for stem induction survival rate of Schisandra sphenanthera Rehd. et Wils.
