中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
14期
2612-2616
,共5页
郇林春%杨新宇%赵旺淼%赵岩%赵杰%张奇%杨树源
郇林春%楊新宇%趙旺淼%趙巖%趙傑%張奇%楊樹源
순림춘%양신우%조왕묘%조암%조걸%장기%양수원
海马%Hes1%神经细胞生成%神经干细胞%免疫组织化学%小鼠
海馬%Hes1%神經細胞生成%神經榦細胞%免疫組織化學%小鼠
해마%Hes1%신경세포생성%신경간세포%면역조직화학%소서
背景:近年来的研究显示,在胚胎神经系统发育过程中,Hes1的表达调控对保持神经干细胞的数量、调控其分化至关重要.此外,成年个体内处于静止期的纤维母细胞重新获得分裂增殖的能力需要Hes1表达的上调.这表明Hes1在成体中也与某些潜在干细胞的增殖具有密切的关系.因此研究Hes1在成体神经干细胞中的表达及其与成体神经细胞生成的关系也就被提上日程.但Hes1在成年个体神经系统的表达至今未明.目的:观察和分析小鼠脑中不同部位Hes1的表达及Hes1阳性细胞的细胞类型.方法:12只成年雄性C57BL/6小鼠随机数字表法分为单染组和双染组,每组6只.单染组商接取材,采用免疫组织化学技术检测Hes1在小鼠脑中各部位的表达.双染组小鼠以200 mg/kg Brdu的剂量每天腹腔注射1次,连续注射3 d,第4天取材,采用双标记物染色观察分析海马区Hes1阳性细胞的细胞类型.结果与结论:在所有观察的解剖部位中,Hes1表达于所有存在神经细胞的部位,Brdu阳性细胞几乎全部表达Hes1,NeuN阳性细胞全部表达Hes1,而神经胶质原纤维酸性蛋白阳性细胞则完全不表达Hes1.由此可知,Hes1表达于神经细胞和神经干细胞中,胶质细胞不表达Hes1.
揹景:近年來的研究顯示,在胚胎神經繫統髮育過程中,Hes1的錶達調控對保持神經榦細胞的數量、調控其分化至關重要.此外,成年箇體內處于靜止期的纖維母細胞重新穫得分裂增殖的能力需要Hes1錶達的上調.這錶明Hes1在成體中也與某些潛在榦細胞的增殖具有密切的關繫.因此研究Hes1在成體神經榦細胞中的錶達及其與成體神經細胞生成的關繫也就被提上日程.但Hes1在成年箇體神經繫統的錶達至今未明.目的:觀察和分析小鼠腦中不同部位Hes1的錶達及Hes1暘性細胞的細胞類型.方法:12隻成年雄性C57BL/6小鼠隨機數字錶法分為單染組和雙染組,每組6隻.單染組商接取材,採用免疫組織化學技術檢測Hes1在小鼠腦中各部位的錶達.雙染組小鼠以200 mg/kg Brdu的劑量每天腹腔註射1次,連續註射3 d,第4天取材,採用雙標記物染色觀察分析海馬區Hes1暘性細胞的細胞類型.結果與結論:在所有觀察的解剖部位中,Hes1錶達于所有存在神經細胞的部位,Brdu暘性細胞幾乎全部錶達Hes1,NeuN暘性細胞全部錶達Hes1,而神經膠質原纖維痠性蛋白暘性細胞則完全不錶達Hes1.由此可知,Hes1錶達于神經細胞和神經榦細胞中,膠質細胞不錶達Hes1.
배경:근년래적연구현시,재배태신경계통발육과정중,Hes1적표체조공대보지신경간세포적수량、조공기분화지관중요.차외,성년개체내처우정지기적섬유모세포중신획득분렬증식적능력수요Hes1표체적상조.저표명Hes1재성체중야여모사잠재간세포적증식구유밀절적관계.인차연구Hes1재성체신경간세포중적표체급기여성체신경세포생성적관계야취피제상일정.단Hes1재성년개체신경계통적표체지금미명.목적:관찰화분석소서뇌중불동부위Hes1적표체급Hes1양성세포적세포류형.방법:12지성년웅성C57BL/6소서수궤수자표법분위단염조화쌍염조,매조6지.단염조상접취재,채용면역조직화학기술검측Hes1재소서뇌중각부위적표체.쌍염조소서이200 mg/kg Brdu적제량매천복강주사1차,련속주사3 d,제4천취재,채용쌍표기물염색관찰분석해마구Hes1양성세포적세포류형.결과여결론:재소유관찰적해부부위중,Hes1표체우소유존재신경세포적부위,Brdu양성세포궤호전부표체Hes1,NeuN양성세포전부표체Hes1,이신경효질원섬유산성단백양성세포칙완전불표체Hes1.유차가지,Hes1표체우신경세포화신경간세포중,효질세포불표체Hes1.
BACKGROUND:In recent years,it has been shown in studies that regulation of the expression of Has1 is very important to maintain neural stem cells and regulate its differentiation during the development of embryonic nervous system.Moreover,upregulation of Hes1 expression is required for quiescent adult flbroblasts resuming proliferation.This indicates that there is a close relationship between Hes1 and some potential stem cells in adult.Therefore,the study of Hes1 expression in neural stem cells and its relationship with adult neurogenasis is put on the agenda.However,the expression of Hes1 in adult brain is still not clear.OBJECTIVE:To observe the expression of Hea1 in adult mouse brain and the cell types of Has1 positive cells.METHODS:Totally 12 adult male C57BL/6 mice were randomly evenly divided into two groups by using the random number table.For the first group,the mouse brain was removed directly and immunohistochemical staining was used to detect the expression of Has1 in the adult mouse brain.For the second group,Brdu was injected intraperitoneally to every mouse with a dosage of 200 mg/kg,once a day,for 3 days.The mouse brain was removed at 4 days and then double staining was carded out to observe and analysis the cell types of Hes1 positive cells in hippocampus.RESULTS AND CONCLUSION:Hes1 was expressed in all the observed anatomical site where neural cells exist,Brdu positive cells nearly all expressed Hea1,NeuN positive cells all expressed Has1,whereas GFAP positive cells did not express Hes1 completely.Hes1 was expressed in all neurons and neural stem cells,and glial cells did not express Hes1.
