CAJ | 학술논문

目的观察PI3K/Akt在高糖诱导的肾小管上皮细胞转分化中的作用及对分化抑制因子2(Id2)表达的影响.方法大鼠肾小管上皮细胞随机分成3组:对照(Con)组,高糖(H-Glu)组和PI3K抑制剂Wortmannin(Wort)组.Con组加正常培养液,H-Glu组在培养液中加30 mmol/L葡萄糖,Wort组用Wortmannin预处理1 h后,加30 mmol/L葡萄糖.24 h后用免疫细胞化学、Western blot法和RT-PCR法检测α平滑肌肌动蛋白(α-SMA)、p-Akt和Id2的表达.结果 Con组α-SMA、p-Akt阳性表达细胞很少,绝大部分细胞出现Id2阳性表达.与Con组比较,H-Glu组出现α-SMA和p-Akt表达升高,Id2表达降低(P<0.05).与H-Glu组比较,Wort组α-SMA和p-Akt表达下降,Id2表达升高(P<0.05).结论 PI3K/Akt可能通过抑制Id2基因表达,参与高糖诱导的大鼠肾小管上皮细胞转分化.
목적 관찰PI3K/Akt재고당유도적신소관상피세포전분화중적작용급대분화억제인자2(Id2)표체적영향.방법 대서신소관상피세포수궤분성3조:대조(Con)조,고당(H-Glu)조화PI3K억제제Wortmannin(Wort)조.Con조가정상배양액,H-Glu조재배양액중가30 mmol/L포도당,Wort조용Wortmannin예처리1 h후,가30 mmol/L포도당.24 h후용면역세포화학、Western blot법화RT-PCR법검측α평활기기동단백(α-SMA)、p-Akt화Id2적표체.결과 Con조α-SMA、p-Akt양성표체세포흔소,절대부분세포출현Id2양성표체.여Con조비교,H-Glu조출현α-SMA화p-Akt표체승고,Id2표체강저(P<0.05).여H-Glu조비교,Wort조α-SMA화p-Akt표체하강,Id2표체승고(P<0.05).결론 PI3K/Akt가능통과억제Id2기인표체,삼여고당유도적대서신소관상피세포전분화.
Objective To observe the effects of PI3K/Akt on inhibitor of differentiation 2 in tubular epithelial-myofibroblast transition induced by high glucose.MethodsNormal rat kidney tubular epithelial cells were randomly divided into three groups:control group(group Con),high glucose group (group H-Glu) and wortmannin group(group Wort). Cells in group Wort were pretreated with wortmannin for an hour before stimulation with high glucose ( 30mmol/L) in group H-Glu and Wort, and cells in group Con were treated with media only. Twenty four hours later, the level of phosphorylation Akt ,Id2 and expression of α-SMA were assayed.ResultsExpression of p-Akt and α-SMA were very low and most of the cell had positive expression of Id2 in group Con. In group H-Glu the expression of α-SMA and p-Akt increased and Id2 decreased significantly, while in group Wort the wortmannin obviously alleviated the change induced by high glucose. Conclusions PI3K/Akt may involve in tubular epithelial-myofibroblast transition induced by high glucose through inhibiting Id2. 　