中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2001年
2期
101-104
,共4页
汪斌超%李龙芸%姚连昌%刘丽华%朱元珏
汪斌超%李龍蕓%姚連昌%劉麗華%硃元玨
왕빈초%리룡예%요련창%류려화%주원각
基因,p53%肺肿瘤%诊断%痰
基因,p53%肺腫瘤%診斷%痰
기인,p53%폐종류%진단%담
目的 评价痰标本检测p53基因点突变方法及其作为肺癌早期临床诊断监测指标的真实性和可靠性。方法 用聚合酶链反应(PCR)-单链多肽性(SSCP)-银染法检测54例原发性肺癌患者和114例良性肺疾病患者痰标本中p53基因第5~8外显子的点突变,同时进行痰涂片细胞学检查。结果 p53突变在肺癌组检出率为55.56%(30/54),非肺癌组检出率为1.75%(2/114),P<0.001。痰标本检测p53基因突变作为肺癌临床诊断监测指标的灵敏性为55.56%,特异性为98.25%,阳性似然比为31.75。肺癌组p53阳性检出率(55.56%)与痰涂片瘤细胞阳性检出率(35.19%)比较,差异有显著性(P<0.05),关联性有极显著意义(P<0.01)。肺癌组p53检出率与性别、吸烟指数、病理分型、疾病分期均无明显关系,但与年龄有密切关系,高龄患者(≥60岁)检出率高(P=0.02)。结论 PCR-SSCP-银染法检测痰标本p53突变可以在可疑肺癌患者(如吸烟并慢性肺疾患者)中作为一项随访监测指标,并将有助于肺癌的早期临床诊断。
目的 評價痰標本檢測p53基因點突變方法及其作為肺癌早期臨床診斷鑑測指標的真實性和可靠性。方法 用聚閤酶鏈反應(PCR)-單鏈多肽性(SSCP)-銀染法檢測54例原髮性肺癌患者和114例良性肺疾病患者痰標本中p53基因第5~8外顯子的點突變,同時進行痰塗片細胞學檢查。結果 p53突變在肺癌組檢齣率為55.56%(30/54),非肺癌組檢齣率為1.75%(2/114),P<0.001。痰標本檢測p53基因突變作為肺癌臨床診斷鑑測指標的靈敏性為55.56%,特異性為98.25%,暘性似然比為31.75。肺癌組p53暘性檢齣率(55.56%)與痰塗片瘤細胞暘性檢齣率(35.19%)比較,差異有顯著性(P<0.05),關聯性有極顯著意義(P<0.01)。肺癌組p53檢齣率與性彆、吸煙指數、病理分型、疾病分期均無明顯關繫,但與年齡有密切關繫,高齡患者(≥60歲)檢齣率高(P=0.02)。結論 PCR-SSCP-銀染法檢測痰標本p53突變可以在可疑肺癌患者(如吸煙併慢性肺疾患者)中作為一項隨訪鑑測指標,併將有助于肺癌的早期臨床診斷。
목적 평개담표본검측p53기인점돌변방법급기작위폐암조기림상진단감측지표적진실성화가고성。방법 용취합매련반응(PCR)-단련다태성(SSCP)-은염법검측54례원발성폐암환자화114례량성폐질병환자담표본중p53기인제5~8외현자적점돌변,동시진행담도편세포학검사。결과 p53돌변재폐암조검출솔위55.56%(30/54),비폐암조검출솔위1.75%(2/114),P<0.001。담표본검측p53기인돌변작위폐암림상진단감측지표적령민성위55.56%,특이성위98.25%,양성사연비위31.75。폐암조p53양성검출솔(55.56%)여담도편류세포양성검출솔(35.19%)비교,차이유현저성(P<0.05),관련성유겁현저의의(P<0.01)。폐암조p53검출솔여성별、흡연지수、병리분형、질병분기균무명현관계,단여년령유밀절관계,고령환자(≥60세)검출솔고(P=0.02)。결론 PCR-SSCP-은염법검측담표본p53돌변가이재가의폐암환자(여흡연병만성폐질환자)중작위일항수방감측지표,병장유조우폐암적조기림상진단。
Objectives To evaluate the value of detecting point mutation of p53 gene in sputum sample and its validity and reliability as a surveillance index in early diagnosis of lung cancer in suspicious patients. Methods Sputum samples were collected from 54 cases identified as lung cancer and 114 cases as pulmonary benign disease. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed for detection of point mutation at exon 5~8 of p53 gene and sputum smear also used for each sample. Results A detection rate of 55. 56% (30/54) in the lung cancer group is significantly higher than that of 1.75% (2/114) in the control (P<0.001). In the lung cancer group, the sensitivity, specificity, and positive likelihood ratio of p53 alterations as a diagnostic marker of lung cancer were 55. 56%, 98. 25% and 31. 75 respectively. Moreover, the detection rates were 35. 19% (19/54) by smears and 55. 56% (30/54) by PCR-SSCP-silver stain ; both the difference and consistency were statistically significant (P<0.01 and P<0.05 respectively). Statistic analysis did not show any significant relation between p53 alteration and clinical parameters such as gender, smoking habits, histotypes and stages, but the detection rate of p53 alteration in older patients (≥60 years old ) was significantly higher than that in younger (P=0. 02). One case with p53 alteration at exon 5 in the control group was confirmed to be squamous carcinoma after 4 years of follow-up. Conclusion Detection of p53 gene alteration in sputum sample by PCR-SSCP-silver stain can be used as a follow-up surveillance index for early diagnosis of lung cancer in suspicious patients.