国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2012年
9期
713-717
,共5页
薛栋%左凯%李新军%王建强%张成德%成丕光%张同军%巩本刚
薛棟%左凱%李新軍%王建彊%張成德%成丕光%張同軍%鞏本剛
설동%좌개%리신군%왕건강%장성덕%성비광%장동군%공본강
肝胰管壶腹%肿瘤%Survivin%环氧化酶-2%免疫组织化学
肝胰管壺腹%腫瘤%Survivin%環氧化酶-2%免疫組織化學
간이관호복%종류%Survivin%배양화매-2%면역조직화학
Ampulla of vater%Neoplasms%Survivin%Cyclooxygenase 2%Immunohischemistry
目的 探讨Survivin和环氧合酶-2(COX-2)蛋白在壶腹癌组织中的表达及其临床意义.方法 用免疫组化EnVision法对40例壶腹癌组织进行Survivin和COX-2蛋白检测,并对8例正常壶腹组织进行对照研究.结果 壶腹癌组织中33例Survivin蛋白呈阳性表达,阳性率为82.5％,对照组中无阳性表达,两组阳性率比较,差异有统计学意义(P＜0.01)；Survivin表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性(均P ＜0.05).在壶腹癌组中27例COX-2蛋白阳性表达,阳性率为67.5％,而对照组8例无阳性表达,两组阳性率比较差异有统计学意义(P＜0.01)；COX-2表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性(均P ＜0.05).经Spearman等级相关性分析表明,Survivin与COX-2蛋白表达呈正相关(r=0.383,P=0.015).结论 Survivin基因和COX-2基因的异常表达可能对壶腹癌的发生和发展起重要作用,可能为壶腹癌的早期诊断和基因治疗提供新的靶点.
目的 探討Survivin和環氧閤酶-2(COX-2)蛋白在壺腹癌組織中的錶達及其臨床意義.方法 用免疫組化EnVision法對40例壺腹癌組織進行Survivin和COX-2蛋白檢測,併對8例正常壺腹組織進行對照研究.結果 壺腹癌組織中33例Survivin蛋白呈暘性錶達,暘性率為82.5％,對照組中無暘性錶達,兩組暘性率比較,差異有統計學意義(P＜0.01)；Survivin錶達與十二指腸浸潤、胰腺浸潤、淋巴結轉移均有明顯相關性(均P ＜0.05).在壺腹癌組中27例COX-2蛋白暘性錶達,暘性率為67.5％,而對照組8例無暘性錶達,兩組暘性率比較差異有統計學意義(P＜0.01)；COX-2錶達與十二指腸浸潤、胰腺浸潤、淋巴結轉移均有明顯相關性(均P ＜0.05).經Spearman等級相關性分析錶明,Survivin與COX-2蛋白錶達呈正相關(r=0.383,P=0.015).結論 Survivin基因和COX-2基因的異常錶達可能對壺腹癌的髮生和髮展起重要作用,可能為壺腹癌的早期診斷和基因治療提供新的靶點.
목적 탐토Survivin화배양합매-2(COX-2)단백재호복암조직중적표체급기림상의의.방법 용면역조화EnVision법대40례호복암조직진행Survivin화COX-2단백검측,병대8례정상호복조직진행대조연구.결과 호복암조직중33례Survivin단백정양성표체,양성솔위82.5％,대조조중무양성표체,량조양성솔비교,차이유통계학의의(P＜0.01)；Survivin표체여십이지장침윤、이선침윤、림파결전이균유명현상관성(균P ＜0.05).재호복암조중27례COX-2단백양성표체,양성솔위67.5％,이대조조8례무양성표체,량조양성솔비교차이유통계학의의(P＜0.01)；COX-2표체여십이지장침윤、이선침윤、림파결전이균유명현상관성(균P ＜0.05).경Spearman등급상관성분석표명,Survivin여COX-2단백표체정정상관(r=0.383,P=0.015).결론 Survivin기인화COX-2기인적이상표체가능대호복암적발생화발전기중요작용,가능위호복암적조기진단화기인치료제공신적파점.
Objective To study the expression of Survivin and COX-2 in ampullary carcinoma and their clinical significance.Methods The expression of Survivin and COX-2 proteins were tested by EnVision immunohistochemistry in 40 cases of ampullary carcinomas,and 8 cases of normal ampulla of vater as the controls.Results The positive rate of Survivin in ampullary carcinonas was significantly higher than that of the controls(82.5％ vs 0,P ＜ 0.01 ). The expression of Survivin in ampullary carcinoma was correlated with duodenal invasion,pancreatic invasion and lymph node metastasis ( P ＜ 0.05 ).Significant difference was also observed in the expression rate of COX-2 between the patients with ampullary carcinoma and the normal controls (67.5％ vs 0,P ＜ 0.01 ).The expression of COX-2 in ampullary carcinoma was correlated with duodenal invasion,pancreatic invasion and lymph node metastasis (P ＜ 0.05). Significantly positive correlation was found between the expression of Survivin and COX-2 by using spearman correlation analysis ( r =0.383,P =0.015).Conclusion The specific up-regulation of COX-2 gene and Survivin gene may play an important role in the genesis and development of ampullary carcinoma.COX-2 and Survivin may be used as early diagnosis markers and potential therapeutic targets in ampullary carcinoma.
