CAJ | 학술논문

目的探讨PRODH基因的rs385440与广西壮族、汉族精神分裂症易感性及其临床症状严重程度之间的关联性,以进一步探明壮、汉族精神分裂症的遗传机制.方法本研究采用病例对照研究方法,以ICD-10为诊断标准,纳入精神分裂症患者282例(壮族94例、汉族188例),健康对照282例(壮族94例、汉族188例).使用TaqMan MGB荧光定量实时PCR实验方法对PRODH基因的rs385440进行基因分型.使用PANSS对246例精神分裂症患者(壮族83例、汉族163例)的临床精神病性症状进行评定.使用SPSS13.0 for windows软件进行统计分析.结果分别在壮族样本、汉族样本以及两个民族的合并样本中,rs385440不同基因型频率、不同等位基因频率在病例组和对照组之间的分布差异均无统计学意义(P＞0.05).在壮族精神分裂症病例中,A等位基因携带者的N4(被动或淡漠社交)条目分[(3.28 ±1.34)分]显著高于G等位基因携带者[(2.40±1.36)分],A等位基因携带者的G12(判断和自知力缺乏)条目为[(4.92±1.55)分]也显著高于G等位基因携带者[(4.12±1.85)分],均差异有统计学意义(P＜0.05).结论 PRODH基因的rs385440与壮族、汉族精神分裂症的易感性都不相关联,但rs385440与壮族精神分裂症的“被动或淡漠社交”、“判断和自知力缺乏”症状严重程度相关联.
목적 탐토PRODH기인적rs385440여엄서장족、한족정신분렬증역감성급기림상증상엄중정도지간적관련성,이진일보탐명장、한족정신분렬증적유전궤제.방법 본연구채용병례대조연구방법,이ICD-10위진단표준,납입정신분렬증환자282례(장족94례、한족188례),건강대조282례(장족94례、한족188례).사용TaqMan MGB형광정량실시PCR실험방법대PRODH기인적rs385440진행기인분형.사용PANSS대246례정신분렬증환자(장족83례、한족163례)적림상정신병성증상진행평정.사용SPSS13.0 for windows연건진행통계분석.결과 분별재장족양본、한족양본이급량개민족적합병양본중,rs385440불동기인형빈솔、불동등위기인빈솔재병례조화대조조지간적분포차이균무통계학의의(P＞0.05).재장족정신분렬증병례중,A등위기인휴대자적N4(피동혹담막사교)조목분[(3.28 ±1.34)분]현저고우G등위기인휴대자[(2.40±1.36)분],A등위기인휴대자적G12(판단화자지력결핍)조목위[(4.92±1.55)분]야현저고우G등위기인휴대자[(4.12±1.85)분],균차이유통계학의의(P＜0.05).결론 PRODH기인적rs385440여장족、한족정신분렬증적역감성도불상관련,단rs385440여장족정신분렬증적“피동혹담막사교”、“판단화자지력결핍”증상엄중정도상관련.
Objective To test the the association between PRODH gene variant rs385440 and the susceptibility to schizophrenia and the severity of schizophrenia in Guangxi Zhuang and Han population,further exploring the genetic mechanisms of schizophrenia in Guangxi Zhuang and Han population.Methods The schizophrenia patients were diagnosed according to ICD-10 criteria in this study.The subjects in the association analysis were 282 unrelated schizophrenia patients(94 Zhuang and 188 Han) and 282 healthy controls (94 Zhuang and 188 Han).A quantitative real-time PCR TaqMan MGB experimental method was carried out to analysis rs385440.The clinical psychotic symptoms of 246 schizophrenia patients (83 Zhuang and 163 Han) were assessed by PANSS.Statistical analyses were carried out with SPSS13.0 for windows.Results There was no statistically significant difference in different allele and genotype frequencies of rs385440 between schizophrenia cases and controls in Zhuang samples,Han samples and combined samples respectively (P＞ 0.05 ).In Zhuang schizophrenia patients the score of N4 (passive/apathetic social withdrawal) item in A allele carriers (3.28 ± 1.34) was higher than that of G allele carriers ( 2.40 ± 1.36 ) significantly (P ＜ 0.05 ),and the score of G12 ( lack of judgment and insight) item in A allele carriers(4.92 ± 1.55 ) was higher than that of G allele carriers ( 4.12 ± 1.85 ) significantly (P ＜ 0.05 ).Conclusion There is no association between PRODH gene variant rs385440 and the susceptibility to schizophrenia in Guangxi Zhuang and Han population.Rs385440 associated the severity of passive/apathetic social withdrawal symptom and poor attention symptom of schizophrenia in Zhuang.