中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
2期
139-142
,共4页
陆雪%封江彬%陈德清%查永如%邹剑明%刘青杰
陸雪%封江彬%陳德清%查永如%鄒劍明%劉青傑
륙설%봉강빈%진덕청%사영여%추검명%류청걸
天然高本底地区%老年居民%EB病毒%永生细胞系%B淋巴细胞
天然高本底地區%老年居民%EB病毒%永生細胞繫%B淋巴細胞
천연고본저지구%노년거민%EB병독%영생세포계%B림파세포
High background radiation area%Old male residents%Epstein-Barr virus%Immortalized cell lines%B-lymphocytes
目的 建立辐射高本底地区老年居民外周血永生化细胞系,以保存高本底居民特有的基因组资源,为进一步开展天然本底辐射的遗传学和分子生物学研究提供材料.方法 在知情同意的情况下收集20例高本底地区老年男性居民的外周血样品,采用EB病毒转化技术,把外周血B淋巴细胞转化成永生化淋巴母细胞系,冻存复苏后,进行细胞活性检测和排除细菌、支原体污染,并用遗传学方法检测其建系前后的遗传稳定性.结果 成功建立20例40株细胞株,所有建成的细胞株冻存后复苏成功率为100%.活性细胞大于90%,无细菌和支原体污染,G显带分析结果为转化后第20代细胞的染色体核型未发生改变.结论 本研究建立的高本底地区老年居民永生细胞系在遗传学特性上是稳定的,可用于进一步的天然本底辐射的遗传学和分子生物学研究.
目的 建立輻射高本底地區老年居民外週血永生化細胞繫,以保存高本底居民特有的基因組資源,為進一步開展天然本底輻射的遺傳學和分子生物學研究提供材料.方法 在知情同意的情況下收集20例高本底地區老年男性居民的外週血樣品,採用EB病毒轉化技術,把外週血B淋巴細胞轉化成永生化淋巴母細胞繫,凍存複囌後,進行細胞活性檢測和排除細菌、支原體汙染,併用遺傳學方法檢測其建繫前後的遺傳穩定性.結果 成功建立20例40株細胞株,所有建成的細胞株凍存後複囌成功率為100%.活性細胞大于90%,無細菌和支原體汙染,G顯帶分析結果為轉化後第20代細胞的染色體覈型未髮生改變.結論 本研究建立的高本底地區老年居民永生細胞繫在遺傳學特性上是穩定的,可用于進一步的天然本底輻射的遺傳學和分子生物學研究.
목적 건립복사고본저지구노년거민외주혈영생화세포계,이보존고본저거민특유적기인조자원,위진일보개전천연본저복사적유전학화분자생물학연구제공재료.방법 재지정동의적정황하수집20례고본저지구노년남성거민적외주혈양품,채용EB병독전화기술,파외주혈B림파세포전화성영생화림파모세포계,동존복소후,진행세포활성검측화배제세균、지원체오염,병용유전학방법검측기건계전후적유전은정성.결과 성공건립20례40주세포주,소유건성적세포주동존후복소성공솔위100%.활성세포대우90%,무세균화지원체오염,G현대분석결과위전화후제20대세포적염색체핵형미발생개변.결론 본연구건립적고본저지구노년거민영생세포계재유전학특성상시은정적,가용우진일보적천연본저복사적유전학화분자생물학연구.
Objective To establish the immortalized cell lines of peripheral blood lymphocytes for old male residents in high background radiation area(HBRA)in Guangdong,China,in order to preserve the specific genomic resources of residents in HBRA for the further genetic and molecular biological study on HBRA.Methods The peripheral blood samples of 20 old male residents in HBRA were collected after informed consent.The immortalized B lymphoblastoid cell lines,2 for each resident,were established with Epstein-Barr virus.After being frozen and recovered,the cell viability,the contamination of bacterium and mycoplaama were analyzed.The stabilization of cell lines was decided by comparing the karyotypes of the peripheral blood lymphocytes and the cell lines.Results 40 cell lines for 20 residents in HBRA were successfully established.The recovery rate of cell lines after being frozen was 100%.All the cell viablity after recovery was higher than 90%.and no contamination of bacteria and mycoplasma occurred.The karyotypes of the 20th generation cell lines were not change.Conclusion The immortalized cell lines established in this study could provide biological resources for further study on genetics and molecular biology in HBRA.
