CAJ | 학술논문

目的探讨维生素D受体、雌激素受体基因型与慢性牙周炎的相关性,比较宿主携带的各基因型对慢性牙周炎临床指标的影响.方法将106例中、重度慢性牙周炎患者(牙周炎组)及80名牙周健康者(对照组)作为研究对象,采用Florida探针检测牙周炎患者的牙周探诊深度、临床附着丧失、龈沟出血指数、松动度等临床指标;抽取所有研究对象的前臂静脉血,饱和NaCl法提取DNA后使用聚合酶链反应(PCR)和酶切相结合的方法检测牙周炎组及对照组维生素D受体基因和雌激素受体基因的多态性,Z检验和方差分析进行数据分析.结果慢性牙周炎患者携带维生素D受体BB基因型的个体百分比为43.4%,健康对照组为30.0%;慢性牙周炎患者携带雌激素受体XX基因型的个体百分比为39.6%,健康对照组为20.0%,差异有统计学意义(P<0.01).同时携带维生素D受体BB基因型、雌激素受体XX基因型的个体牙周状况较携带其他基因型的个体差.结论维生素D受体等位基因B、雌激素受体等位基因X是中、重度慢性牙周炎的易感等位基因;这两种等位基因的共同携带对慢性牙周炎的病情进展有一定的促进作用.
목적 탐토유생소D수체、자격소수체기인형여만성아주염적상관성,비교숙주휴대적각기인형대만성아주염림상지표적영향.방법 장106례중、중도만성아주염환자(아주염조)급80명아주건강자(대조조)작위연구대상,채용Florida탐침검측아주염환자적아주탐진심도、림상부착상실、간구출혈지수、송동도등림상지표;추취소유연구대상적전비정맥혈,포화NaCl법제취DNA후사용취합매련반응(PCR)화매절상결합적방법검측아주염조급대조조유생소D수체기인화자격소수체기인적다태성,Z검험화방차분석진행수거분석.결과 만성아주염환자휴대유생소D수체BB기인형적개체백분비위43.4%,건강대조조위30.0%;만성아주염환자휴대자격소수체XX기인형적개체백분비위39.6%,건강대조조위20.0%,차이유통계학의의(P<0.01).동시휴대유생소D수체BB기인형、자격소수체XX기인형적개체아주상황교휴대기타기인형적개체차.결론 유생소D수체등위기인B、자격소수체등위기인X시중、중도만성아주염적역감등위기인;저량충등위기인적공동휴대대만성아주염적병정진전유일정적촉진작용.
Objective To investigate the relationship between chronic periodontitis and the genetic polymorphisms of vitamin D receptor gene and estrogen receptor gene.Methods Clinical parameters including probing depth,clinical attachment loss,sulcus bleeding index and tooth movement were measured by floride probe.Genomic DNA from peripheral venous blood was extracted with saturant sodium chloride,and PCR-restriction fragment length polymorphism was applied to examine the Apa Ⅰ,Bsm Ⅰ,Taq Ⅰpolymorphisms of the vitamin D receptor genes and the Xba Ⅰ and PvuⅡpolymorphisms of the estrogen receptor genes.The results were analyzed by Z-score test and mean square analysis.Results Forty-three point four percent of chronic periodontitis patients took vitamin D receptor BB genotype,the rate in healthy controls was 30.0%.39.6% of chronic periodontitis patients took estrogen receptor XX genotype,the rate in healthy controls was 20.0%.The people who took BBXX genotype had the worst periodontal conditions among all chronic periodontitis patients.Conclusions Vitamin D receptor allele B and estrogen receptor allele X ale susceptible alleles for chronic periodontitis.The synergistic effects of the two receptor susceptible alleles may promote chronic periodontitis.