CAJ | 학술논문

目的探讨干扰素(IFN)应答相关基因在预测丙型肝炎抗病毒疗效中的作用.方法采用功能分类基因芯片方法对IFN治疗前慢性丙型肝炎患者外周血单个核细胞(PBMC)IFN相关基因的表达进行检测,结合临床资料进行分析.采用SPSS 12.0软件进行统计学处理.结果 17例慢性丙型肝炎患者中,IFN治疗快速病毒学应答者(RVR)10例,非快速病毒学应答者(N-RVR)7例.与健康对照组比较,RVR患者PBMC中发现9个差异表达基因,其中1个为上调基因,8个为下调基因;N-RVR患者有18个差异表达基因,均为下调基因.RVR与N-RVR患者比较,发现5个差异表达基因,其中4个为上调基因,分别为PRKCZ、PRKRA、IRF5和TNFSF10(t=5.44、3.13、5.24和2.30,P=0.000、0.010、0.005和0.044),1个下调基因为IFIT5(t=2.43,P=0.035).17例患者中,12例为HCV1b型,5例为HCV2a型,HCV1b型与HCV2a型患者比较,发现2个下调基因,分别为IFI6和IFI44(t=2.42和2.45,P=0.038和0.033).结论 IFN应答相关基因下调可能与慢性丙型肝炎患者对IFN治疗的应答有关.HCV1b型患者比HCV2a型患者更容易诱导IFN相关基因表达的下调,从而产生IFN抵抗.
목적 탐토간우소(IFN)응답상관기인재예측병형간염항병독료효중적작용.방법 채용공능분류기인심편방법대IFN치료전만성병형간염환자외주혈단개핵세포(PBMC)IFN상관기인적표체진행검측,결합림상자료진행분석.채용SPSS 12.0연건진행통계학처리.결과 17례만성병형간염환자중,IFN치료쾌속병독학응답자(RVR)10례,비쾌속병독학응답자(N-RVR)7례.여건강대조조비교,RVR환자PBMC중발현9개차이표체기인,기중1개위상조기인,8개위하조기인;N-RVR환자유18개차이표체기인,균위하조기인.RVR여N-RVR환자비교,발현5개차이표체기인,기중4개위상조기인,분별위PRKCZ、PRKRA、IRF5화TNFSF10(t=5.44、3.13、5.24화2.30,P=0.000、0.010、0.005화0.044),1개하조기인위IFIT5(t=2.43,P=0.035).17례환자중,12례위HCV1b형,5례위HCV2a형,HCV1b형여HCV2a형환자비교,발현2개하조기인,분별위IFI6화IFI44(t=2.42화2.45,P=0.038화0.033).결론 IFN응답상관기인하조가능여만성병형간염환자대IFN치료적응답유관.HCV1b형환자비HCV2a형환자경용역유도IFN상관기인표체적하조,종이산생IFN저항.
Objective To explore the application of interferon(IFN)response-related genes in predicting the outcome of antiviral treatment in chronic hepatitis C. Methods SuperArray microarray was used to detect the expression of IFN response-related genes in peripheral blood monocytes(PBMC)from chronic hepatitis C patients before treatment. SPSS 12.0 was used for statistical analysis. Results Ten patients were classified as rapid responders(RVR)and seven patients as non-RVRs according to the serum HCV RNA level after 4 weeks of treatment in 17 patients. Compared with healthy controls, nine differentially expressed genes were found in RVR patients, one up-regulated and eight down-regulated; eighteen differentially expressed genes were found in N-RVR patients, all down-regulated. Five differentially expressed genes were found between the patients with RVR and N-RVR: four up-regulated genes were PRKCZ, PRKRA, IRF5 and TNFSF10(t =5.44, 3.13, 5.24 and 2.30, P=0. 000, 0.010, 0.005 and 0. 044); one up-regulated gene was IFIT5(t = 2.43, P = 0. 035). Of seventeen patients, 12 were HCV genotype 1b, 5 were HCV genotype 2a. Compared with HCV2a, IFI6 and IFI44 gene in HCV1b were downregulated(t = 2.42 and 2.45, P = 0. 038 and 0. 033). Conclusions The expression of IFN responserelated genes is associated with response to IFN treatment. HCV genotype 1 b is more successful in inducing the down-regulation of IFN response-related genes than that of HCV genotype 2a, thus leading to the resistance to IFN.