中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2011年
4期
399-402
,共4页
王婷婷%张亚楼%刘继文%王生玲
王婷婷%張亞樓%劉繼文%王生玲
왕정정%장아루%류계문%왕생령
亚砷酸盐类%磷脂酰胆碱类%细胞膜%Na+,K+交换ATP酶
亞砷痠鹽類%燐脂酰膽堿類%細胞膜%Na+,K+交換ATP酶
아신산염류%린지선담감류%세포막%Na+,K+교환ATP매
Arsenites%Phosphatidylcholines%Cell membrane%Na+-K+-exchanging ATPase
目的 观察卵磷脂对亚砷酸钠(NaAsO2)染毒非洲绿猴肾细胞(Vero)细胞膜损伤的作用.方法 将体外培养Vero细胞分为4组:对照组(生理盐水)、砷模型组(2.20 mg/L NaAsO2)、卵磷脂高剂量+砷干预组(53.33 mg/L卵磷脂+2.20 mg/L NaAsO2)、卵磷脂低剂量+砷干预组(13.32 mg/L卵磷脂+2.20 mg/L NaAsO2),每组6瓶细胞,每2天换液1次,培养120 h.采用分光光度法测定细胞膜Na+,K+-ATP酶活性,高效液相法测定细胞膜磷脂组分磷脂酰丝氨酸(PS)、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)和神经鞘磷脂(SM).结果 对照组、砷模型组、卵磷脂高剂量+砷干预组、卵磷脂低剂量+砷干预组细胞膜Na+,K+-ATP酶活性分别为(O.962 ±0.081)×106、(0.544±0.037)×106、(0.647±0.043)×106、(0.550±0.020)× 106 U·kg-1·h-1,各组间比较,差异有统计学意义(F=43.58,P<0.01).与对照组比较,其他3组细胞膜Na+,K+-ATP酶活性明显降低(P均<0.05);与砷模型组比较,卵磷脂高剂量+砷干预组明显增高(P<0.05),而卵磷脂低剂量+砷十预组未见明显改变(P>0.05).与对照组[(0.087±0.003)、(0.127±0.053)、(0.588±0.105)、(0.07l±0.029)g/L]比较,砷模型组PS、PE、PC、SM水平[(0.051±0.018)、(0.073±0.030)、(0.240 4-0.038)、(0.047±0.121)g/L]均明显降低(P均<0.05);卵磷脂高剂量+砷干预组PS、PE、PCI(0.084±0.011)、(0.109±0.363)、(0.591±0.476)g/L]未见明显改变(P均>0.05),而SM[(0.057±0.004)g/L]明显降低(P<0.05);卵磷脂低剂量+砷干预组PS、PE、SM[(0.058±0.020)、(0.086±0.177)、(0.048±0.103)g/L]明显降低(P均<0.05),而PCI(0.521±0.098)g/L]未见明显改变(P>0.05).与砷模型组比较,卵磷脂高剂量+砷干预组PS、PE、PC、SM明显增高(P均<0.05);卵磷脂低剂量+砷干预组PS、PE、SM未见明显改变(P均>0.05),PC明显增高(P<0.05).结论 高剂量卵磷脂对砷染毒Vero细胞膜损伤具有一定的保护作用.
目的 觀察卵燐脂對亞砷痠鈉(NaAsO2)染毒非洲綠猴腎細胞(Vero)細胞膜損傷的作用.方法 將體外培養Vero細胞分為4組:對照組(生理鹽水)、砷模型組(2.20 mg/L NaAsO2)、卵燐脂高劑量+砷榦預組(53.33 mg/L卵燐脂+2.20 mg/L NaAsO2)、卵燐脂低劑量+砷榦預組(13.32 mg/L卵燐脂+2.20 mg/L NaAsO2),每組6瓶細胞,每2天換液1次,培養120 h.採用分光光度法測定細胞膜Na+,K+-ATP酶活性,高效液相法測定細胞膜燐脂組分燐脂酰絲氨痠(PS)、燐脂酰乙醇胺(PE)、燐脂酰膽堿(PC)和神經鞘燐脂(SM).結果 對照組、砷模型組、卵燐脂高劑量+砷榦預組、卵燐脂低劑量+砷榦預組細胞膜Na+,K+-ATP酶活性分彆為(O.962 ±0.081)×106、(0.544±0.037)×106、(0.647±0.043)×106、(0.550±0.020)× 106 U·kg-1·h-1,各組間比較,差異有統計學意義(F=43.58,P<0.01).與對照組比較,其他3組細胞膜Na+,K+-ATP酶活性明顯降低(P均<0.05);與砷模型組比較,卵燐脂高劑量+砷榦預組明顯增高(P<0.05),而卵燐脂低劑量+砷十預組未見明顯改變(P>0.05).與對照組[(0.087±0.003)、(0.127±0.053)、(0.588±0.105)、(0.07l±0.029)g/L]比較,砷模型組PS、PE、PC、SM水平[(0.051±0.018)、(0.073±0.030)、(0.240 4-0.038)、(0.047±0.121)g/L]均明顯降低(P均<0.05);卵燐脂高劑量+砷榦預組PS、PE、PCI(0.084±0.011)、(0.109±0.363)、(0.591±0.476)g/L]未見明顯改變(P均>0.05),而SM[(0.057±0.004)g/L]明顯降低(P<0.05);卵燐脂低劑量+砷榦預組PS、PE、SM[(0.058±0.020)、(0.086±0.177)、(0.048±0.103)g/L]明顯降低(P均<0.05),而PCI(0.521±0.098)g/L]未見明顯改變(P>0.05).與砷模型組比較,卵燐脂高劑量+砷榦預組PS、PE、PC、SM明顯增高(P均<0.05);卵燐脂低劑量+砷榦預組PS、PE、SM未見明顯改變(P均>0.05),PC明顯增高(P<0.05).結論 高劑量卵燐脂對砷染毒Vero細胞膜損傷具有一定的保護作用.
목적 관찰란린지대아신산납(NaAsO2)염독비주록후신세포(Vero)세포막손상적작용.방법 장체외배양Vero세포분위4조:대조조(생리염수)、신모형조(2.20 mg/L NaAsO2)、란린지고제량+신간예조(53.33 mg/L란린지+2.20 mg/L NaAsO2)、란린지저제량+신간예조(13.32 mg/L란린지+2.20 mg/L NaAsO2),매조6병세포,매2천환액1차,배양120 h.채용분광광도법측정세포막Na+,K+-ATP매활성,고효액상법측정세포막린지조분린지선사안산(PS)、린지선을순알(PE)、린지선담감(PC)화신경초린지(SM).결과 대조조、신모형조、란린지고제량+신간예조、란린지저제량+신간예조세포막Na+,K+-ATP매활성분별위(O.962 ±0.081)×106、(0.544±0.037)×106、(0.647±0.043)×106、(0.550±0.020)× 106 U·kg-1·h-1,각조간비교,차이유통계학의의(F=43.58,P<0.01).여대조조비교,기타3조세포막Na+,K+-ATP매활성명현강저(P균<0.05);여신모형조비교,란린지고제량+신간예조명현증고(P<0.05),이란린지저제량+신십예조미견명현개변(P>0.05).여대조조[(0.087±0.003)、(0.127±0.053)、(0.588±0.105)、(0.07l±0.029)g/L]비교,신모형조PS、PE、PC、SM수평[(0.051±0.018)、(0.073±0.030)、(0.240 4-0.038)、(0.047±0.121)g/L]균명현강저(P균<0.05);란린지고제량+신간예조PS、PE、PCI(0.084±0.011)、(0.109±0.363)、(0.591±0.476)g/L]미견명현개변(P균>0.05),이SM[(0.057±0.004)g/L]명현강저(P<0.05);란린지저제량+신간예조PS、PE、SM[(0.058±0.020)、(0.086±0.177)、(0.048±0.103)g/L]명현강저(P균<0.05),이PCI(0.521±0.098)g/L]미견명현개변(P>0.05).여신모형조비교,란린지고제량+신간예조PS、PE、PC、SM명현증고(P균<0.05);란린지저제량+신간예조PS、PE、SM미견명현개변(P균>0.05),PC명현증고(P<0.05).결론 고제량란린지대신염독Vero세포막손상구유일정적보호작용.
Objective To observe the lecithin's effect on membrane of African green monkey kidney cells (Vero) exposed to sodium arsenite(NaAsO2). Methods Vero cells cultured in vitro were divided into 4 groups:control group (saline), model group (2.20 mg/L NaAsO2), high eoncentration of lecithin and arsenic group (53.33mg/L lecithin + 2.20 mg/L NaAsO2), low eoncentration of lecithin and arsenic group( 13.32 mg/L lecithin + 2.20 mg/L NaAsO2), 6 bottles of cells in each group, medium was changed every 2 days, cultured for 120 h. Na+ ,K+-ATPase activities of membrane were measured by spectrophotometry, and membrane phospholipids composition including phosphatidylserine (PS), phosphatidylethano-lamine (PE), phosphatidylcholine (PC) and sphingmyelin (SM) were measured by high performance liquid chromatography (HPLC). Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P < 0.05). Compared with model group, the Na+ ,K+-ATPase activity in high concentration of lecithin and arsenic group was significantly higher (P < 0.05),but in low concentration of lecithin and arsenic group did not change significantly (P > 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P < 0.05) ;PS, PE, PC in high concentration of lecithin and arsenic group[(0.084 ± 0.011), (0.109 ± 0.363), (0.591 ± 0.476)g/L] did not change significantly(all P > 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P < 0.05) ;PS, PE, SM levels of low concentration of lecithin and arsenic group[(0.058 ± 0.020), (0.086 ± 0.177), (0.048 ± 0.103)g/L] significantly reduced (all P < 0.05), the PC did not change significantly [(0.521±0.098 )g/L, P > 0.05]. Compared with model group,the levels of PS, PE, PC, SM in high concentration of lecithin and arsenic group were significantly higher(all P <0.05);PS, PE, SM levels in low concentration of lecithin and arsenic group did not change significantly(all P > 0.05), and PC was significantly higher(P < 0.05). Conclusions High concentration lecithin has certain protective effect on Vero cell membrane exposured to sodium arsenite.
