CAJ | 학술논문

目的制备β神经生长因子(nerve growth factor,NGF)缓释系统,评价其及其组份对体外培养鸡胚背根神经节(dorsal root ganglion,DRG)轴突生长的生物学效应.方法构建含β-NGF 50、100、250μL的缓释系统分别与DRG共同培养,DRG常规培养作为对照组,了解各浓度组DRG的轴突生长;分别利用缓释系统各组份与DRG培养,观察各组轴突生长情况.实验分成6组:对照组(DRG+纤维蛋白原),A组(DRG+纤维蛋白原+肝素结合肽+肝素钠+100 μg/Lβ-NGF),B组(DRG+纤维蛋白原+肝素钠+100μg/Lβ-NCF),C组(DRG+纤维蛋白原+肝素结合肽+100μg/Lβ-NGF),D组(DRG+纤维蛋白原+100μg/Lβ-NGF),E组(DRG+纤维蛋白原+肝素结合肽十肝素钠).结果 50、100和250μg/Lβ-NGF缓释系统轴突生长是对照组的1.31(P>0.05)、3.78(P<0.01)和3.05(P<0.01)倍;100μg/L组较2.50μg/L组DRG轴突生长明显(P<0.05).实验组A、B、C、D和E组的DRG轴突生长分别是对照组的3.75、1.15、1.12、1.10和1.09倍,与对照组比较仅A组差异有统计学意义(P<0.01).结论 β-NGF缓释系统中释放的β-NGF具有生物活性,可明显促进DRG轴突生长.
목적 제비β신경생장인자(nerve growth factor,NGF)완석계통,평개기급기조빈대체외배양계배배근신경절(dorsal root ganglion,DRG)축돌생장적생물학효응.방법 구건함β-NGF 50、100、250μL적완석계통분별여DRG공동배양,DRG상규배양작위대조조,료해각농도조DRG적축돌생장;분별이용완석계통각조빈여DRG배양,관찰각조축돌생장정황.실험분성6조:대조조(DRG+섬유단백원),A조(DRG+섬유단백원+간소결합태+간소납+100 μg/Lβ-NGF),B조(DRG+섬유단백원+간소납+100μg/Lβ-NCF),C조(DRG+섬유단백원+간소결합태+100μg/Lβ-NGF),D조(DRG+섬유단백원+100μg/Lβ-NGF),E조(DRG+섬유단백원+간소결합태십간소납).결과 50、100화250μg/Lβ-NGF완석계통축돌생장시대조조적1.31(P>0.05)、3.78(P<0.01)화3.05(P<0.01)배;100μg/L조교2.50μg/L조DRG축돌생장명현(P<0.05).실험조A、B、C、D화E조적DRG축돌생장분별시대조조적3.75、1.15、1.12、1.10화1.09배,여대조조비교부A조차이유통계학의의(P<0.01).결론 β-NGF완석계통중석방적β-NGF구유생물활성,가명현촉진DRG축돌생장.
Objective To construct β-NGF controlled delivery system and evaluate the biological effects of β-NGF on the growth of chick embryo dorsal root ganglion (DRG) axons in vitro. Methods Delivery systems releasing β-NGF at 50/μL, 100/μg/L and 250 μg/L concentration were constructed. To determine the optimal dose response effects of NGF in the controlled delivery system, DRG were co-cultured with of β-NGF at above concentrations while using DRG basic culture as control. Axonal growth was observed. DRG were also cocultured with the components in the controlled delivery system to detect the effects on growth of DRG axons. The experiment was divided into 5 experimental groups and 1 control group: control group, DRG+ fibrin; Group A,DRG+ fibrin+ peptide + heparin + 100 μg/L β-NGF; Group B, DRG + fibrin + heparin + 100/μg/L β-NGF;Group C, DRG + fibrin + peptide + 100 μg/L β-NGF; Group D, DRG + fibrin + 100 μg/L β-NGF; Group E,DRG + fibrin + peptide + heparin. Results The growth of DRG axons in 50 μg/L, 100μg/L and 250/μg/Lconcentration of β-NGF controlled delivery system was 1.31 ( P > 0. 05), 3.78 ( P < 0. 01 ) and 3.05 ( P <0.01) folds of the control respectively. The growth of DRG axons in 100 μg/L group was significantly better comparing to that in 250 μg/L group. The growth of DRG axons in Groups A, B, C, D and E was 3.75, 1.15,1.12, 1.10 and 1.09 folds of the control group, respectively. The difference was only statistically significant between Group A and the control group ( P < 0. 01 ). Conclusion β-NGF released from the β-NGF controlled delivery system was bioactive. It could promote the growth of DRG axons.