CAJ | 학술논문

目的研究胃癌患者肿瘤坏死因子(TNF)-α308和TNF-β252基因型与血清TNF-α、β水平的关系.方法收集病理诊断证实的57例胃癌患者,49例来白武汉大学中南医院,8例来自湖北省肿瘤医院.同时选取年龄与性别相匹配的18名健康体检者作为对照.采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法检测患者外周静脉血.采用ELISA方法检测57例胃癌患者及18名对照者的血清TNF-α、β水平,比较胃癌患者各TNF基因型之间血清TNF水平的差异及其与对照者血清TNF水平的差异.分析胃癌患者TNF水平与临床病理特征的关系.结果胃癌患者总体血清TNF-α水平较对照组明显增高(中位数445×10-3 μg/L比5×10-3 μg/L,P＜0.05),而且TNF-α308和TNF-β252各基因型(TNF α308 GA基因型6例、GG基因型51例,TNF-β252 GG基因型17例,GA和AA基因型各20例)的血清TNF-α水平较对照组也明显增高(P＜0.05),但血清总体TNF-β水平与对照组比较差异无统计学意义(P＞0.05).此外,TNF α308G/TNF-β252G和TNF-α308G/TNF-β252A单倍型胃癌患者血清TNF-α水平较对照组也明显增高(P＜0.05),其增高与血清总体TNF-α水平一样,与患者年龄、淋巴结转移显著相关(P＜0.05).TNF-α308A和TNF-β252G的高危等位基因携带者的TNF β水平与吸烟史显著相关(P＜0.05).结论胃癌患者血清TNF-α水平增高与TNF-α308和TNF-β252基因型无显著相关性.TNF- α308G/TNF-β252G和TNF-d308G/TNF- β252A单倍型胃癌患者的血清TNF-α水平增高与年龄、淋巴结转移显著相关,提示TNF基因单倍型对胃癌TNF的表达及临床类型可能具有一定的影响.
목적 연구위암환자종류배사인자(TNF)-α308화TNF-β252기인형여혈청TNF-α、β수평적관계.방법 수집병리진단증실적57례위암환자,49례래백무한대학중남의원,8례래자호북성종류의원.동시선취년령여성별상필배적18명건강체검자작위대조.채용한제성편단장도다태성취합매련반응(PCR-RFLP)방법검측환자외주정맥혈.채용ELISA방법검측57례위암환자급18명대조자적혈청TNF-α、β수평,비교위암환자각TNF기인형지간혈청TNF수평적차이급기여대조자혈청TNF수평적차이.분석위암환자TNF수평여림상병리특정적관계.결과 위암환자총체혈청TNF-α수평교대조조명현증고(중위수445×10-3 μg/L비5×10-3 μg/L,P＜0.05),이차TNF-α308화TNF-β252각기인형(TNF α308 GA기인형6례、GG기인형51례,TNF-β252 GG기인형17례,GA화AA기인형각20례)적혈청TNF-α수평교대조조야명현증고(P＜0.05),단혈청총체TNF-β수평여대조조비교차이무통계학의의(P＞0.05).차외,TNF α308G/TNF-β252G화TNF-α308G/TNF-β252A단배형위암환자혈청TNF-α수평교대조조야명현증고(P＜0.05),기증고여혈청총체TNF-α수평일양,여환자년령、림파결전이현저상관(P＜0.05).TNF-α308A화TNF-β252G적고위등위기인휴대자적TNF β수평여흡연사현저상관(P＜0.05).결론 위암환자혈청TNF-α수평증고여TNF-α308화TNF-β252기인형무현저상관성.TNF- α308G/TNF-β252G화TNF-d308G/TNF- β252A단배형위암환자적혈청TNF-α수평증고여년령、림파결전이현저상관,제시TNF기인단배형대위암TNF적표체급림상류형가능구유일정적영향.
Objective To investigate the relationship between tumor necrosis factor (TNF) α308,TNF-β252 genotypes and serum TNF-α and TNF-β levels in patients with gastric cancer (GC).Methods A total of 57 pathological diagnosed GC patients were collected,of which 49 cases were from Zhongnan Hospital of Wuhan University and 8 cases were from Tumor Hospital of Hubei Province.Another 18 age and sex matched healthy controls were enrolled at the same time.The TNF-α308 and TNF -β252 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The serum TNF-α and TNF-β levels of 57 GC patients and 18 healthy controls were measured by ELISA.The difference of TNF serum level in different TNF genotypes of GC and the difference between GC patients and the healthy controls was analyzed.Its relationship with clinical pathological characters was also analyzed.Results With TNF-α308 genotype,GA were 6 cases and GG were 51 cases.With TNF-β252 genotype,GG were 17 cases,GA and AA each were 20 cases.The serum TNF-α level of GC patients was significant higher than that of healthy controls (median 445 × 10-3 μg/L vs 5 × 10-3 μg/L,P＜0.05),and the serum level of each TNF-α308 and TNF-β 252 genotypes was significant higher than that of healthy controls (P＜0.05).However there was no statistical significance in TNF -β level compared with healthy controls (P＞0.05).In addition,the serum TNF-α levels of the TNF-α308G/TNF-β252G and TNF-α308G/TNF β252A haplotypes in GC patients were significant higher than those of the healthy controls (P＜0.05),and the increase like serum TNF-α level was associated with the patients'age and lymph node metastasis (P＜0.05).The TNF-β level in patients with TNF-α308A and TNF-β252G high-risk haplotypes showed a significant relation with smoking history (P＜ 0.05). Conclusions Serum TNF-α level of GC patients was significantly higher,however there was no significant association between the increase and TNF-α308 and TNF-β252 genotypes.The serum TNF-α levels of TNF-α308G/TNF-β252G and TNF-α308G/TNF-β252A haplotypes in GC patients were significant higher,and associated with the patients'age and lympb node metastasis.It was indicated that TNF haplotypes may have certain impact on the TNF expression and clinical subtypes in GC.