中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
8期
735-739
,共5页
张欣%倪汉忠%管大伟%邹丽容%周杰%侯年妹%朱晓兰%肖红%柯昌文%武婕
張訢%倪漢忠%管大偉%鄒麗容%週傑%侯年妹%硃曉蘭%肖紅%柯昌文%武婕
장흔%예한충%관대위%추려용%주걸%후년매%주효란%초홍%가창문%무첩
甲型H1N1流感病毒%血凝素基因%测序%进化
甲型H1N1流感病毒%血凝素基因%測序%進化
갑형H1N1류감병독%혈응소기인%측서%진화
Pandemic H1N1 influenza viruses%Hemagglutinin gene%Sequencing%Evolution
目的 了解2009-2011年广东甲型H1N1流感病毒血凝素基因的HA1进化特征。方法 选取广东甲型H1N1流感病毒83株，提取病毒RNA，经RT-PCR反应扩增HA1并测序，测定的序列用生物信息软件分析，与GenBank中相关序列比较，并对推导的编码氨基酸序列进行进化分析。结果 2009-2011年广东甲型H1N1流感病毒HA1基因的进化速率是5.2× 10-3,高于人季节性H1N1病毒；变异氨基酸多数位于HA蛋白表面，其中部分位于抗原决定簇；在两例死亡病例分离株HA1的第222位氨基酸发生D222G/D222N变异。结论 遗传进化分析表明，甲型H1N1流感病毒发生了一定程度的变异，造成2011年初在广东的再次流行。HA1的第222位氨基酸变异可能与疾病的严重程度有关。
目的 瞭解2009-2011年廣東甲型H1N1流感病毒血凝素基因的HA1進化特徵。方法 選取廣東甲型H1N1流感病毒83株，提取病毒RNA，經RT-PCR反應擴增HA1併測序，測定的序列用生物信息軟件分析，與GenBank中相關序列比較，併對推導的編碼氨基痠序列進行進化分析。結果 2009-2011年廣東甲型H1N1流感病毒HA1基因的進化速率是5.2× 10-3,高于人季節性H1N1病毒；變異氨基痠多數位于HA蛋白錶麵，其中部分位于抗原決定簇；在兩例死亡病例分離株HA1的第222位氨基痠髮生D222G/D222N變異。結論 遺傳進化分析錶明，甲型H1N1流感病毒髮生瞭一定程度的變異，造成2011年初在廣東的再次流行。HA1的第222位氨基痠變異可能與疾病的嚴重程度有關。
목적 료해2009-2011년엄동갑형H1N1류감병독혈응소기인적HA1진화특정。방법 선취엄동갑형H1N1류감병독83주，제취병독RNA，경RT-PCR반응확증HA1병측서，측정적서렬용생물신식연건분석，여GenBank중상관서렬비교，병대추도적편마안기산서렬진행진화분석。결과 2009-2011년엄동갑형H1N1류감병독HA1기인적진화속솔시5.2× 10-3,고우인계절성H1N1병독；변이안기산다수위우HA단백표면，기중부분위우항원결정족；재량례사망병례분리주HA1적제222위안기산발생D222G/D222N변이。결론 유전진화분석표명，갑형H1N1류감병독발생료일정정도적변이，조성2011년초재엄동적재차류행。HA1적제222위안기산변이가능여질병적엄중정도유관。
Objective To understand the evolutionary characterization of hemagglutinin (HA)gene of pandemic H1N1 influenza virus in Guangdong during 2009-2011. Methods We selected 83 pandemic H1N1 strains isolated in Guangdong during 2009-2011. The HA1 genes were sequenced and analyzed comparatively by Bioedit 7.0 and MEGA 4.0. Results The evolutionary rate of Hal gene of pandemic H1N1 and seasonal H1N1 viruses was 5.2×10-3 substitutions/site/year, higher than that of seasonal H1N1 viruses. Most amino acid changes in HA1 molecules accumulated on the surface of the molecule and were partly located in antigenic sites. Two fatal infections were detected with a mutation at HA residue 222, in one virus with a change D222G, and in one virus D222N. Conclusion The phylogenetic analysis demonstrates that the influenza epidemic in Guangdong at the beginning of 2011 are due to occurrence of genetic changes of pandemic H1N1 virus. The amino acid change at residue 222 of the HA1 are likely to be associated with severe or even fatal illness.
