CAJ | 학술논문

目的建立一种新型的体外胆管灌注系统模型,探讨各种胆管内试验在这种模型里进行同质对比研究的可行性.方法设计一种10个样品试验区的体外胆管灌注系统模型,将生理盐水、甘油和猪胆汁分别放入这种模型里,将第一个样品区的流量设定在8滴/min,在0.5、1.0、1.5、2.0、12.0和24.0h末收集并测量这种模型的10个样品区流出的液体量,评估这些液体量在每一个时间点是否相等.10种含依地酸二钠不同含量的、相同规格的缓释药棒分别放入这种模型的试验区,在注入这些液体后,在24.0h末收集并分析这10个试验区的依地酸二钠浓度,评估它们是否相等.同时,评估这3种液体对同一种药物缓释棒是否相等.结果在相同时间点,流经10个样品试验区的生理盐水、甘油和猪胆汁是相等的,差异无统计学意义(P＞0.01)；但是对于同一种液体,依地酸二钠浓度在24.0h末不同(P＜0.01)；对于同一种药物缓释棒,不同的液体也有差异(P＜0.01).结论这种模型能够获得相同流量和实验环境,当做不同的实验时能够在不同的试验区获得不同结果,当实验环境发生改变时同一种样品的结果也会发生相应的改变,可以模拟胆管环境进行体外试验研究.
목적 건립일충신형적체외담관관주계통모형,탐토각충담관내시험재저충모형리진행동질대비연구적가행성.방법 설계일충10개양품시험구적체외담관관주계통모형,장생리염수、감유화저담즙분별방입저충모형리,장제일개양품구적류량설정재8적/min,재0.5、1.0、1.5、2.0、12.0화24.0h말수집병측량저충모형적10개양품구류출적액체량,평고저사액체량재매일개시간점시부상등.10충함의지산이납불동함량적、상동규격적완석약봉분별방입저충모형적시험구,재주입저사액체후,재24.0h말수집병분석저10개시험구적의지산이납농도,평고타문시부상등.동시,평고저3충액체대동일충약물완석봉시부상등.결과 재상동시간점,류경10개양품시험구적생리염수、감유화저담즙시상등적,차이무통계학의의(P＞0.01)；단시대우동일충액체,의지산이납농도재24.0h말불동(P＜0.01)；대우동일충약물완석봉,불동적액체야유차이(P＜0.01).결론 저충모형능구획득상동류량화실험배경,당주불동적실험시능구재불동적시험구획득불동결과,당실험배경발생개변시동일충양품적결과야회발생상응적개변,가이모의담관배경진행체외시험연구.
Objective To establish a new model of biliary peffusion in vitro,and to explore the feasibility of comparative biliary studies with the model.Methods A set of ten-text-area model of biliary peffusion was designed in vitro.Saline,glycerol and pig bile were put into this model.The outflow of the first tube was set at 8 drops/min,and these liquids were collected through the ten sample test areas and measured at 0.5 hour,1.0 hour,1.5 hours,2.0 hours,12.0 hours and 24.0 hours.Equality of liquid amount was evaluated from each sample test area.Rods containing delayed release EDTA of ten different levels were placed in the test areas.The liquid was collected and EDTA concentrations were evaluated from the ten tubes at 24.0 hours.EDTA concentrations of the same rod in the three liquids were compared.Results A(1) the same time point,the amounts of saline,glycerol,pig bile flowing through the ten sample test areas showed no significant difference (P ＞ 0.01 ) ; but EDTA concentrations of the same liquid at 24.0 hours were significantly different (P ＜ 0.01 ) ; and EDTA concentrations of the same rod in the three liquids were also obviously different ( P ＜ 0.01 ).Conclusion The same liquid flow rates and the same experimental environment can be achieved.Different test results can be obtained from different test areas in different experiments.The test results of the same kind of sample change with the changes of experimental conditions,which simulates biliary duct.