CAJ | 학술논문

目的探讨不同方法检测胃癌中Her-2蛋白表达和基因表达的相关性,寻求胃癌中Her-2检测的有效手段.方法应用免疫组织化学方法(IHC)对68例胃癌进行Her-2蛋白标记,同时采用荧光原位杂交技术(FISH)检测Her-2基因扩增的情况.结果 68例胃癌患者中,IHC检测Her-2蛋白阳性12例(1765％),分别+++3例,++9例、+7例和-49例；FISH检测Her-2基因扩增11例(16.17％),与IHC阳性强度相对应的FISH检测结果分别为3例(100％)、8例(88.89％)、0例、0例.Her-2基因扩增与Her-2蛋白表达存在相关性(P＜0.05).结论胃癌中存在Her-2蛋白的表达和基因扩增,Her-2蛋白在胃癌中的表达呈现明显的异质性.IHC与FISH检测胃癌Her-2的表达差异主要在IHC++组,IHC++有意愿进行Her-2基因靶向治疗的患者建议进行Her-2基因检测.
목적 탐토불동방법검측위암중Her-2단백표체화기인표체적상관성,심구위암중Her-2검측적유효수단.방법 응용면역조직화학방법(IHC)대68례위암진행Her-2단백표기,동시채용형광원위잡교기술(FISH)검측Her-2기인확증적정황.결과 68례위암환자중,IHC검측Her-2단백양성12례(1765％),분별+++3례,++9례、+7례화-49례；FISH검측Her-2기인확증11례(16.17％),여IHC양성강도상대응적FISH검측결과분별위3례(100％)、8례(88.89％)、0례、0례.Her-2기인확증여Her-2단백표체존재상관성(P＜0.05).결론 위암중존재Her-2단백적표체화기인확증,Her-2단백재위암중적표체정현명현적이질성.IHC여FISH검측위암Her-2적표체차이주요재IHC++조,IHC++유의원진행Her-2기인파향치료적환자건의진행Her-2기인검측.
Objective To investigate the association of Her-2 protein expression and gene expression in gastric cancer by different methods,and to explore the effective approaches of Her-2 in gastric cancer detection.Methods Immunohistochemistry (IHC) was used to mark Her-2 protein in 68 cases of gastric cancer,and fluorescence in situ hybridization(FISH)wasused to detect the Her-2 gene amplification.Results Among 68 cases,IHC Her-2 protein in 12 cases(17.65 ％)was positive expression,including ++ 3 cases ++ 9 cases,+ 7 cases and-49 cases.By FISH,Her-2 gene in 11 cases(16.17 ％)was overexpression.Corresponding to the IHC results,FISH results was 3 cases(100 ％),8 cases(88.89 ％),0 cases,0 cases,respectively.Conclusion Her-2 protein expression and gene overexpression occur in gastric cancer.Her-2 protein expression in gastric cancer shows significant heterogeneity.Her-2 gene overexpression and Her-2 protein expression is correlated (P＜0.05).The differences of Her-2 expression in gastric cancer by IHC and FISH mostly occur in IHC++ groupe.Suggestion of Her-2 gene test should be given for patients with IHC++ who will be undertaken Her-2 gene therapy.