食品工程
食品工程
식품공정
FOOD ENGINEERING
2009年
2期
51-53
,共3页
鱼肉%鱼鳞%蛋白质%靛酚蓝比色分光光度法
魚肉%魚鱗%蛋白質%靛酚藍比色分光光度法
어육%어린%단백질%전분람비색분광광도법
fish flesh%fish scale%protein%indophenol blue spectrophotometry
采用靛酚蓝分光光度法对两种鱼的鱼肉和鱼鳞中的蛋白质含量进行了比较测定.结果表明该法稳定性、重复性好.该法最大吸收波长为648nm,摩尔吸光系数E648=1.209 L·mg-1·cm-1,标准曲线相关系数为r=0.9956.溶液经2 h后可完全显色,2 h后直至24 h吸光度无显著变化,平均回收率为98.9%.
採用靛酚藍分光光度法對兩種魚的魚肉和魚鱗中的蛋白質含量進行瞭比較測定.結果錶明該法穩定性、重複性好.該法最大吸收波長為648nm,摩爾吸光繫數E648=1.209 L·mg-1·cm-1,標準麯線相關繫數為r=0.9956.溶液經2 h後可完全顯色,2 h後直至24 h吸光度無顯著變化,平均迴收率為98.9%.
채용전분람분광광도법대량충어적어육화어린중적단백질함량진행료비교측정.결과표명해법은정성、중복성호.해법최대흡수파장위648nm,마이흡광계수E648=1.209 L·mg-1·cm-1,표준곡선상관계수위r=0.9956.용액경2 h후가완전현색,2 h후직지24 h흡광도무현저변화,평균회수솔위98.9%.
The comparative determination of proteins in fish flesh and fish scale by Indophenol Blue Spectropho-tometry was studied. The result showed this method has good stability and repeatability. The maximum absorption wavelength of colored complex was found to be at 648 nm with apparent molar absorption coefficient E648 was 1.209 L·mg-1·cm-1.The coefficient of standard curve was r=0.9956. Coloration time was 2 hours and within 2～24hours it has stable coloration. The average recovery rate was 98.9%.
