CAJ | 학술논문

背景:以组织工程气管作为移植气管,不但能降低抗原细胞的表达,抑制免疫反应,还能够预先在体外进行气管的血管化以及相关组织细胞的再生培养.目的:总结归纳常用的气管移植的方法,介绍组织工程气管的最新临床移植方式,为开展该类手术提供参考依据.方法:以tissue engineering,trachea,transplantation为检索词,检索Pubmed数据库(1990-01/2009-05),ScienceDirect数据库(1990-01/2009-05),Springer数据库(1990-01/2009-05);以组织工程学,气管,移植为检索词,检索CNKI数据库(1990-01/2009-05).文献检索语种限制为英文和中文.纳入与气管移植相关的临床研究和动物实验;排除重复文献.结果与结论:计算机初检得到270篇文献,根据纳入排除标准,对其中32篇文献进行分析.由于各种原因引起的气管不可逆性损伤且需移植置换的情况在临床工作中日趋增多,而移植气管的材料来源一直是制约临床气管移植的主要问题.体外构建组织工程气管用于移植被视为气管修复的有效途径.文章对气管移植及组织工程气管的起源与发展进行回顾,且对近年来较常用的同种异体移植,自体组织移植及生物材料移植等方法,生物支架材料的构建研究,种子细胞的选取研究,组织工程气管的临床应用进展等进行简要叙述,并对最新的利用同种异体气管与剔除供者免疫细胞进行种子细胞培养相结合的方法,成功完成全球首例人造气管移植的案例进行着重的介绍.
배경:이조직공정기관작위이식기관,불단능강저항원세포적표체,억제면역반응,환능구예선재체외진행기관적혈관화이급상관조직세포적재생배양.목적:총결귀납상용적기관이식적방법,개소조직공정기관적최신림상이식방식,위개전해류수술제공삼고의거.방법:이tissue engineering,trachea,transplantation위검색사,검색Pubmed수거고(1990-01/2009-05),ScienceDirect수거고(1990-01/2009-05),Springer수거고(1990-01/2009-05);이조직공정학,기관,이식위검색사,검색CNKI수거고(1990-01/2009-05).문헌검색어충한제위영문화중문.납입여기관이식상관적림상연구화동물실험;배제중복문헌.결과여결론:계산궤초검득도270편문헌,근거납입배제표준,대기중32편문헌진행분석.유우각충원인인기적기관불가역성손상차수이식치환적정황재림상공작중일추증다,이이식기관적재료래원일직시제약림상기관이식적주요문제.체외구건조직공정기관용우이식피시위기관수복적유효도경.문장대기관이식급조직공정기관적기원여발전진행회고,차대근년래교상용적동충이체이식,자체조직이식급생물재료이식등방법,생물지가재료적구건연구,충자세포적선취연구,조직공정기관적림상응용진전등진행간요서술,병대최신적이용동충이체기관여척제공자면역세포진행충자세포배양상결합적방법,성공완성전구수례인조기관이식적안례진행착중적개소.
BACKGROUND: Tissue-engineered trachea serving as a tracheal transplant, can not only reduce the expression of antigen cells, suppress immunoreaction, but also in advance conduct vascularization of blood vessels and regeneration culture of tissue cells in vitro. OBJECTIVE: To summarize the commonly used methods for tracheal transplantation, to introduce the latest clinical transplantation method of tissue-engineered trachea, and to provide reference evidence for carrying out such operations. METHODS: Taking tissue engineering, trachea, transplantation in English as search terms, Pubrned database from January 1990 to May 2009, ScienceDirect database from January 1990 to May 2009, Springer database from January 1990 to May 2009 was retrieved; Taking tissue engineering, trachea, transplantation in Chinese as search terms, CNKI database from January 1990 to May 2009 was researched. Literatures were limited to English and Chinese languages. Inclusive criteria: clinical studies and animal experiments related to the tracheal transplantation; exclusive criteria: duplicate documents. RESULTS AND CONCLUSION: Totally 270 literatures were screened out by computer, accordingto inclusion and exclusion criteria, 32 documents of which were involved for analysis. There is an increasing cases with irreversible injury of tracheal resulted from various reasons and in need of transplantation during clinical work, and the material source of tracheal transplantation is always the main problem to limited the clinical use. Constructing tissue-engineered trachea in vitro is considered to be a good way for repairing trachea. This paper will review the origin and development of tracheal transplantation and tissue-engineered trachea, summarize the common method of allotransplantation, autotransplantation and biomaterial transplantation, as well as the research of constructing bio-scaffold, choosing seed cells and clinical applied development of tissue-engineered trachea, and emphatically introduce the first case of successful artificial tracheal transplantation by combining the method of allotransplantation and culturing the seed cell without the immune cells of donor.