中南大学学报(医学版)
中南大學學報(醫學版)
중남대학학보(의학판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY（MEDICAL SCIENCES）
2011年
1期
15-20
,共6页
淫羊藿总黄酮%卵巢切除术%下丘脑%海马%雌激素受体α%雌激素受体β
淫羊藿總黃酮%卵巢切除術%下丘腦%海馬%雌激素受體α%雌激素受體β
음양곽총황동%란소절제술%하구뇌%해마%자격소수체α%자격소수체β
total flavonoids of Epimedium sagittatum (TFE)%ovariectomy(OVX)%hypothalamus%hippocampus%estrogen receptor alpha(ER α)%estrogen receptor beta(ER β)
目的:观察淫羊藿总黄酮对去卵巢大鼠下丘脑和海马雌激素受体α及β mRNA表达的影响,探讨其治疗绝经后骨质疏松的作用机制.方法:将10～11 月龄SD雌性大鼠48只, 随机分为假手术组、去卵巢模型组、淫羊藿总黄酮组和雌二醇(E2)组.以双侧卵巢切除法建立大鼠骨质疏松模型.淫羊藿总黄酮组、E2组分别用淫羊藿总黄酮、E2给药4 个月,采用RT-PCR 法检测各组下丘脑和海马ERα,ERβ mRNA的表达.结果:大鼠卵巢切除后,血E2 水平、椎骨骨密度、子宫湿重、下丘脑和海马ERα,ERβ mRNA的表达均明显下降.E2治疗后血E2水平、椎骨骨密度、子宫湿重升高,下丘脑和海马ERβ mRNA的表达均明显下降, 下丘脑和海马ERβ mRNA的表达无明显改变.淫羊藿总黄酮治疗后,血E2水平、椎骨骨密度、下丘脑和海马ERα,ERβ mRNA的表达均明显升高,子宫湿重无明显改变. 结论:淫羊藿总黄酮对切除卵巢所致的骨质疏松大鼠有防治作用,对子宫无不良反应;其机制可能与其选择性上调下丘脑和海马ERα,ERβ mRNA的表达有关.
目的:觀察淫羊藿總黃酮對去卵巢大鼠下丘腦和海馬雌激素受體α及β mRNA錶達的影響,探討其治療絕經後骨質疏鬆的作用機製.方法:將10～11 月齡SD雌性大鼠48隻, 隨機分為假手術組、去卵巢模型組、淫羊藿總黃酮組和雌二醇(E2)組.以雙側卵巢切除法建立大鼠骨質疏鬆模型.淫羊藿總黃酮組、E2組分彆用淫羊藿總黃酮、E2給藥4 箇月,採用RT-PCR 法檢測各組下丘腦和海馬ERα,ERβ mRNA的錶達.結果:大鼠卵巢切除後,血E2 水平、椎骨骨密度、子宮濕重、下丘腦和海馬ERα,ERβ mRNA的錶達均明顯下降.E2治療後血E2水平、椎骨骨密度、子宮濕重升高,下丘腦和海馬ERβ mRNA的錶達均明顯下降, 下丘腦和海馬ERβ mRNA的錶達無明顯改變.淫羊藿總黃酮治療後,血E2水平、椎骨骨密度、下丘腦和海馬ERα,ERβ mRNA的錶達均明顯升高,子宮濕重無明顯改變. 結論:淫羊藿總黃酮對切除卵巢所緻的骨質疏鬆大鼠有防治作用,對子宮無不良反應;其機製可能與其選擇性上調下丘腦和海馬ERα,ERβ mRNA的錶達有關.
목적:관찰음양곽총황동대거란소대서하구뇌화해마자격소수체α급β mRNA표체적영향,탐토기치료절경후골질소송적작용궤제.방법:장10～11 월령SD자성대서48지, 수궤분위가수술조、거란소모형조、음양곽총황동조화자이순(E2)조.이쌍측란소절제법건립대서골질소송모형.음양곽총황동조、E2조분별용음양곽총황동、E2급약4 개월,채용RT-PCR 법검측각조하구뇌화해마ERα,ERβ mRNA적표체.결과:대서란소절제후,혈E2 수평、추골골밀도、자궁습중、하구뇌화해마ERα,ERβ mRNA적표체균명현하강.E2치료후혈E2수평、추골골밀도、자궁습중승고,하구뇌화해마ERβ mRNA적표체균명현하강, 하구뇌화해마ERβ mRNA적표체무명현개변.음양곽총황동치료후,혈E2수평、추골골밀도、하구뇌화해마ERα,ERβ mRNA적표체균명현승고,자궁습중무명현개변. 결론:음양곽총황동대절제란소소치적골질소송대서유방치작용,대자궁무불량반응;기궤제가능여기선택성상조하구뇌화해마ERα,ERβ mRNA적표체유관.
Objective To explore the effect of total flavonoids of Epimedium sagittatum (TFE) on the mRNA expressions of the estrogen receptor alpha (ERα) and estrogen receptor beta(ERβ) in hippocampus and hypothalamus in ovariectomized (OVX) Sprague-Dawley(SD) rats, and the mechanism of TFE against postmenopausal osteoporosis. Methods Forty-eight female SD rats,aged 10-11 months old, were randomly divided into 4 groups: a sham group, an ovariectomy group (rats were bilaterally ovariectomized), a TFE group, and a 17β-estradiol group (rats were fed with TFE and 17β-estradiol for 4 months, respectively). The RT-PCR was performed to determine the mRNA expression of ERα and ERβ in hypothalamus and hippocampus. Results Serum estradiol level, bone mineral density (BMD) of vertebra,wet weight of uterus, and the mRNA expressions of ERα and ERβ in hypothalamus and hippocampus were markedly decreased in OVX rats, all of which were reversed by 17β-estradiol treatment except the mRNA expression of ERβ. Similar results were achieved by TFE treatment except the wet weight of uterus. Conclusion TFE can improve BMD of vertebra in the OVX rats without side effects on the uterus. The mechanism may be related to increasing the mRNA expressions of ERα and ERβ in hypothalamus and hippocampus.
