国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2008年
5期
307-309
,共3页
谭卓%黄云坚%夏晓华%倪崖%沈小琴%房国坚%李坤
譚卓%黃雲堅%夏曉華%倪崖%瀋小琴%房國堅%李坤
담탁%황운견%하효화%예애%침소금%방국견%리곤
HIV-1%免疫印迹%尿液%血液
HIV-1%免疫印跡%尿液%血液
HIV-1%면역인적%뇨액%혈액
HIV-1%Western blot%Urine%Blood
目的 用免疫印迹法检测HIV携带者的血液和尿液样本,研究尿检试剂替代血检试剂用于HIV确证的可行性.方法 采集河南、浙江研究对象87名的血液和尿液,分别用血检试剂和尿检试剂平行检测,按各自检定标准判定结果,统计各特异性条带出现频率.结果 87名被检者中,血检阳性41人,尿检阳性39人,符合率为97.7%.阳性血液样本中出现频率最高的条带是gp160、p24、gp120,最低的是p55、p31、p17;阳性尿液样本中出现频率最高的条带是gp160、gp120、sp41,最低的是p17、p55、p24.结论 HIV携带者尿液中的抗体与血液不同,灵敏度也有一定差异,通过改进尿检试剂的制备工艺,同时制定适宜的判定标准,可以使尿检试剂替代血检试剂应用于HIV-1抗体的检测确证.
目的 用免疫印跡法檢測HIV攜帶者的血液和尿液樣本,研究尿檢試劑替代血檢試劑用于HIV確證的可行性.方法 採集河南、浙江研究對象87名的血液和尿液,分彆用血檢試劑和尿檢試劑平行檢測,按各自檢定標準判定結果,統計各特異性條帶齣現頻率.結果 87名被檢者中,血檢暘性41人,尿檢暘性39人,符閤率為97.7%.暘性血液樣本中齣現頻率最高的條帶是gp160、p24、gp120,最低的是p55、p31、p17;暘性尿液樣本中齣現頻率最高的條帶是gp160、gp120、sp41,最低的是p17、p55、p24.結論 HIV攜帶者尿液中的抗體與血液不同,靈敏度也有一定差異,通過改進尿檢試劑的製備工藝,同時製定適宜的判定標準,可以使尿檢試劑替代血檢試劑應用于HIV-1抗體的檢測確證.
목적 용면역인적법검측HIV휴대자적혈액화뇨액양본,연구뇨검시제체대혈검시제용우HIV학증적가행성.방법 채집하남、절강연구대상87명적혈액화뇨액,분별용혈검시제화뇨검시제평행검측,안각자검정표준판정결과,통계각특이성조대출현빈솔.결과 87명피검자중,혈검양성41인,뇨검양성39인,부합솔위97.7%.양성혈액양본중출현빈솔최고적조대시gp160、p24、gp120,최저적시p55、p31、p17;양성뇨액양본중출현빈솔최고적조대시gp160、gp120、sp41,최저적시p17、p55、p24.결론 HIV휴대자뇨액중적항체여혈액불동,령민도야유일정차이,통과개진뇨검시제적제비공예,동시제정괄의적판정표준,가이사뇨검시제체대혈검시제응용우HIV-1항체적검측학증.
Objective To test and compare results of HIV-1 antibody in blood and urine specimens tested by western blot. Method 87 blood specimens and paired urine specimens were collected from Henan and Zhejiang, and tested by western blot. The results according to the criterion of each assay were interpreted and the frequencies of virus specific bands were counted. Results Among 87 specimens, 41 blood specimens and 39 mine specimens were positive respectively. The coincidence rate was 97.7%. In blood specimens, the frequency of gp160/p24/gp120 bands was the highest and that of p55/p31/p17 was the lowest. In urine specimens, the frequency of gp160/gp120/gp41 bands was the highest and that of p17/p55/p24 was the lowest. Conclusions HIV-1 antibody in urine is different from that in blood, new technics and criterions will increase the probability of test in urine specimens.