CAJ | 학술논문

目的检测慢性乙型肝炎患者肝组织HBV共价闭合环状DNA (cccDNA)和血清HBsAg,分析两种定量指标之间及其与血清HBV DNA载量的相关性.方法应用PSAD消化+滚环扩增+跨缺口实时荧光PCR方法,定量检测54例慢性乙型肝炎患者甲醛固定石蜡包埋肝组织HBV cccDNA水平；用化学发光试剂定量检测患者血清HBsAg.用Pearson检验及直线回归分析方法对数据进行分析.结果患者肝组织HBV cccDNA与血清HBsAg定量水平之间呈正相关(r=0.459,P＜0.01),但与血清HBV DNA载量相关性无统计学意义；血清HBsAg定量水平与血清HBV DNA载量呈正相关(r=0.328,P＜ 0.05),与病毒复制效率呈负相关(r=-0.373,P＜0.05).结论慢性乙型肝炎患者肝组织HBV cccDNA载量与血清HBsAg定量水平相关,结合血清HBVDNA定量检测,可以更全面的反映HBV的复制水平,评价抗病毒疗效.
목적 검측만성을형간염환자간조직HBV공개폐합배상DNA (cccDNA)화혈청HBsAg,분석량충정량지표지간급기여혈청HBV DNA재량적상관성.방법 응용PSAD소화+곤배확증+과결구실시형광PCR방법,정량검측54례만성을형간염환자갑철고정석사포매간조직HBV cccDNA수평；용화학발광시제정량검측환자혈청HBsAg.용Pearson검험급직선회귀분석방법 대수거진행분석.결과 환자간조직HBV cccDNA여혈청HBsAg정량수평지간정정상관(r=0.459,P＜0.01),단여혈청HBV DNA재량상관성무통계학의의；혈청HBsAg정량수평여혈청HBV DNA재량정정상관(r=0.328,P＜ 0.05),여병독복제효솔정부상관(r=-0.373,P＜0.05).결론 만성을형간염환자간조직HBV cccDNA재량여혈청HBsAg정량수평상관,결합혈청HBVDNA정량검측,가이경전면적반영HBV적복제수평,평개항병독료효.
Objective To quantitatively detect intrahepatic HBV covalently closed circular DNA (cccDNA) and serum HBsAg; and to analyze the relationship between the two parameters and with serum HBV DNA level.Methods Intrahepatic cccDNA (copies/cell) was quantitated by plasmid-safe ATPdependent Danes (PSAD) digestion in combination with rolling circle amplification and gap-spanning selective real-time PCR assay using formalin fixed paraffin-embedded liver biopsy samples.HBsAg was measured by chemiluminescence's reagent manufactured by Abbott Company using sera sampled at timepoint of liver biopsy.Results Intrahepatic cccDNA level was positively correlated with serum HBsAg level (r=0.459,P ＜ 0.001),but not correlated with serum HBV DNA level.Serum HBsAg level was positively correlated with serum HBV DNA level (r =0.328,P =0.015),and reversely correlated with HBV replicative efficiency defined as the ratio of serum HBV DNA to cccDNA (r =-0.373,P =0.005).Conclusion In patients with chronic hepatitis B,intrahepatic cccDNA level is correlated with serum HBsAg level.The two parameters combined with serum HBV DNA may comprehensively reflect HBV replication activity and help evaluation of antiviral therapeutic efficacy.