国际内分泌代谢杂志
國際內分泌代謝雜誌
국제내분비대사잡지
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2012年
2期
80-83
,共4页
杨丽娜%汪蓓蕾%孙亚婷%张瑞%郭刚
楊麗娜%汪蓓蕾%孫亞婷%張瑞%郭剛
양려나%왕배뢰%손아정%장서%곽강
甲状腺激素%GAP-43%大鼠%免疫组织化学%脑发育
甲狀腺激素%GAP-43%大鼠%免疫組織化學%腦髮育
갑상선격소%GAP-43%대서%면역조직화학%뇌발육
Thyroid hormone%GAP- 43%Rat%Immunohistochemistry%Brain development
目的 研究不同孕期低碘孕鼠补充甲状腺激素对子鼠脑组织生长相关蛋白(GAP) - 43表达的影响,探讨甲状腺激素不足造成的脑发育落后现象的可能机制.方法 1月龄Wistar大鼠160只,雌、雄各半.雌性大鼠随机分为8组:正常组和7个低碘组,每组10只.各组均饲以低碘饲料,正常组饮用碘浓度为200 μg/L的碘酸钾溶液,总碘摄入量相当于大鼠生理碘需要量,低碘组饮用去离子水.3个月后,与正常Wistar雄鼠进行交配.选择1个低碘组不补充甲状腺激素作为阴性对照,其余6组分别在妊娠早期(孕1~17 d)和妊娠中、晚期(孕18 d~出生后20 d)补充高、中、低剂量甲状腺激素,剂量分别为:3.5、2、0.5 μg/100 g体重.8组分别取新生当天、生后7d、14 d、21 d、28 d的子鼠脑组织,应用SABC法检测GAP- 43在神经细胞中的表达,图像分析方法定量检测蛋白的含量.结果 低碘组不同日龄子鼠脑组织GAP-43的表达普遍低于同日龄正常组(P<0.05),只有在28 d时大于正常组(P<0.05);对低碘孕鼠给予中、高剂量的甲状腺激素,可以使子鼠脑组织GAP-43的表达接近正常水平,尤其是在孕早期补充甲状腺激素(P<0.05).结论 孕鼠低碘导致子鼠脑组织GAP- 43表达降低,低碘孕鼠补充中剂量以上的甲状腺激素,可以使子代GAP-43的表达水平接近正常,在孕早期补充效果更好.
目的 研究不同孕期低碘孕鼠補充甲狀腺激素對子鼠腦組織生長相關蛋白(GAP) - 43錶達的影響,探討甲狀腺激素不足造成的腦髮育落後現象的可能機製.方法 1月齡Wistar大鼠160隻,雌、雄各半.雌性大鼠隨機分為8組:正常組和7箇低碘組,每組10隻.各組均飼以低碘飼料,正常組飲用碘濃度為200 μg/L的碘痠鉀溶液,總碘攝入量相噹于大鼠生理碘需要量,低碘組飲用去離子水.3箇月後,與正常Wistar雄鼠進行交配.選擇1箇低碘組不補充甲狀腺激素作為陰性對照,其餘6組分彆在妊娠早期(孕1~17 d)和妊娠中、晚期(孕18 d~齣生後20 d)補充高、中、低劑量甲狀腺激素,劑量分彆為:3.5、2、0.5 μg/100 g體重.8組分彆取新生噹天、生後7d、14 d、21 d、28 d的子鼠腦組織,應用SABC法檢測GAP- 43在神經細胞中的錶達,圖像分析方法定量檢測蛋白的含量.結果 低碘組不同日齡子鼠腦組織GAP-43的錶達普遍低于同日齡正常組(P<0.05),隻有在28 d時大于正常組(P<0.05);對低碘孕鼠給予中、高劑量的甲狀腺激素,可以使子鼠腦組織GAP-43的錶達接近正常水平,尤其是在孕早期補充甲狀腺激素(P<0.05).結論 孕鼠低碘導緻子鼠腦組織GAP- 43錶達降低,低碘孕鼠補充中劑量以上的甲狀腺激素,可以使子代GAP-43的錶達水平接近正常,在孕早期補充效果更好.
목적 연구불동잉기저전잉서보충갑상선격소대자서뇌조직생장상관단백(GAP) - 43표체적영향,탐토갑상선격소불족조성적뇌발육락후현상적가능궤제.방법 1월령Wistar대서160지,자、웅각반.자성대서수궤분위8조:정상조화7개저전조,매조10지.각조균사이저전사료,정상조음용전농도위200 μg/L적전산갑용액,총전섭입량상당우대서생리전수요량,저전조음용거리자수.3개월후,여정상Wistar웅서진행교배.선택1개저전조불보충갑상선격소작위음성대조,기여6조분별재임신조기(잉1~17 d)화임신중、만기(잉18 d~출생후20 d)보충고、중、저제량갑상선격소,제량분별위:3.5、2、0.5 μg/100 g체중.8조분별취신생당천、생후7d、14 d、21 d、28 d적자서뇌조직,응용SABC법검측GAP- 43재신경세포중적표체,도상분석방법정량검측단백적함량.결과 저전조불동일령자서뇌조직GAP-43적표체보편저우동일령정상조(P<0.05),지유재28 d시대우정상조(P<0.05);대저전잉서급여중、고제량적갑상선격소,가이사자서뇌조직GAP-43적표체접근정상수평,우기시재잉조기보충갑상선격소(P<0.05).결론 잉서저전도치자서뇌조직GAP- 43표체강저,저전잉서보충중제량이상적갑상선격소,가이사자대GAP-43적표체수평접근정상,재잉조기보충효과경호.
Objects To observe the effects of thyroid hormone supplement in different stages of gestation of low-iodine pregnant rats on expression of growth-associated protein(GAP)-43 in the brain tissue of rat offspring,and explore the possible mechanism of brain development defect caused by thyroid hormone deficiency.Methods There are 160 one-month-old Wistar rats,50% male and 50% female.The female rats were randomly divided into eight groups including normal group and 7 low iodine groups,10 rats per group.Rats were fed with low iodine feed.Normal group were given 200 μg/L KIO3.The total iodine intake is equivalent to rat physiological iodine requirements.Other groups drunk deionized water.After 3 months,female rats were mated with normal male rats.Rats of one low iodine group as negative controls are not supplied with thyroid hormone.The other six groups are differently supplied with high/medium/low doses of thyroid hormone in early gestation (pregnant lst-17th day) groups and late gestation (pregnant 18th day-20th day after birth) groups.High/medium/low doses of thyroid hormone is respectively 3.5,2,0.5 μg/100 g weight.Brain tissues of new-born,7-day,14-day,21-day,28-day offspring in every group were taken for samples.The expression of GAP-43 of every group was detected by immunohistochemistry (SABC) and analysed by image method.Results The expression of GAP-43 of neonatal offspring in low iodine group at different time was lower than that in normal group (P < 0.05 ),only in postnatal 28 d group,GAP-43 expression level is higher( P < 0.05 ).If low iodine pregnant rats continue to be given the medium or high dose of thyroid hormone,the expression of GAP-43 in offspring can reach the normal,especially in the early gestation.Conclusion Pregnant rats at low iodine state will result in the lower expression of GAP-43 in the brain of neonatal offspring.When more than medium dose of thyroid hormone was supplied in low-iodine pregnant rats,the expression of GAP-43 in the brain of neonatal offspring could be close to normal level,especially in early gestation.