中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
9期
831-833
,共3页
刘泽金%郑芳%张真路%范庆坤%王纯
劉澤金%鄭芳%張真路%範慶坤%王純
류택금%정방%장진로%범경곤%왕순
碱性磷酸酶%肌钙蛋白Ⅰ%假阳性反应
堿性燐痠酶%肌鈣蛋白Ⅰ%假暘性反應
감성린산매%기개단백Ⅰ%가양성반응
Alkaline phosphatase%troponin Ⅰ%false positive reactions
目的 评价ALP对cTnI测定的干扰.方法 制备1份正常混合血浆和2份不同浓度cTnI的异常混合血浆,各分成8组,第1组为对照组,另7组加入不同浓度ALP,分别在ACCESS2 (Beckman Coulter公司)及AXSYM(美国Abbott公司)上测定每组标本的cTnI和ALP,并与对照组cTnI比较,评价ALP是否对cTnI测定产生干扰.结果 ACCESS cTnI测定正常血浆时,ALP浓度高达3 716 U/L时测定结果为(0.04±0.01)μgL,与正常血浆组的(0.04±0.01)μg/L比较差异无统计学意义(t=0.40,P>0.05);ALP高于917 U/L时,AXSYM cTnI测定结果为(0.08±0.01)μg/L,高于正常血浆组的(0.04±0.01)μg/L,差异有统计学意义(t=-4.89,P<0.01);ACCESS cTnI测定异常血浆时,ALP浓度高达3 534 U/L时,cTnI测定结果为(13.41±0.17)μg/L,与正常血浆组的(13.48±0.16)μg/L比较,差异无统计学意义(t=0.52,P>0.05).结论 高浓度ALP对ACCESS cTnI测定无干扰,不会导致假阳性结果,但高浓度ALP(917 U/L以上)会造成AXSYM cTnI检测干扰导致假阳性结果.
目的 評價ALP對cTnI測定的榦擾.方法 製備1份正常混閤血漿和2份不同濃度cTnI的異常混閤血漿,各分成8組,第1組為對照組,另7組加入不同濃度ALP,分彆在ACCESS2 (Beckman Coulter公司)及AXSYM(美國Abbott公司)上測定每組標本的cTnI和ALP,併與對照組cTnI比較,評價ALP是否對cTnI測定產生榦擾.結果 ACCESS cTnI測定正常血漿時,ALP濃度高達3 716 U/L時測定結果為(0.04±0.01)μgL,與正常血漿組的(0.04±0.01)μg/L比較差異無統計學意義(t=0.40,P>0.05);ALP高于917 U/L時,AXSYM cTnI測定結果為(0.08±0.01)μg/L,高于正常血漿組的(0.04±0.01)μg/L,差異有統計學意義(t=-4.89,P<0.01);ACCESS cTnI測定異常血漿時,ALP濃度高達3 534 U/L時,cTnI測定結果為(13.41±0.17)μg/L,與正常血漿組的(13.48±0.16)μg/L比較,差異無統計學意義(t=0.52,P>0.05).結論 高濃度ALP對ACCESS cTnI測定無榦擾,不會導緻假暘性結果,但高濃度ALP(917 U/L以上)會造成AXSYM cTnI檢測榦擾導緻假暘性結果.
목적 평개ALP대cTnI측정적간우.방법 제비1빈정상혼합혈장화2빈불동농도cTnI적이상혼합혈장,각분성8조,제1조위대조조,령7조가입불동농도ALP,분별재ACCESS2 (Beckman Coulter공사)급AXSYM(미국Abbott공사)상측정매조표본적cTnI화ALP,병여대조조cTnI비교,평개ALP시부대cTnI측정산생간우.결과 ACCESS cTnI측정정상혈장시,ALP농도고체3 716 U/L시측정결과위(0.04±0.01)μgL,여정상혈장조적(0.04±0.01)μg/L비교차이무통계학의의(t=0.40,P>0.05);ALP고우917 U/L시,AXSYM cTnI측정결과위(0.08±0.01)μg/L,고우정상혈장조적(0.04±0.01)μg/L,차이유통계학의의(t=-4.89,P<0.01);ACCESS cTnI측정이상혈장시,ALP농도고체3 534 U/L시,cTnI측정결과위(13.41±0.17)μg/L,여정상혈장조적(13.48±0.16)μg/L비교,차이무통계학의의(t=0.52,P>0.05).결론 고농도ALP대ACCESS cTnI측정무간우,불회도치가양성결과,단고농도ALP(917 U/L이상)회조성AXSYM cTnI검측간우도치가양성결과.
Objective To evaluate the interference of ALP on cTnI assays. Methods One normal mixed plasma sample and 2 abnormal mixed plasma samples with different cTnI levels were prepared, and then divided them into 8 groups respectively. One group was randomly chosen as control while different amounts of ALP were added into the other seven groups. The concentrations of cTnI and ALP in each plasma portion were detected by ACCESS2 (Beckman-Coulter, Inc ) and AXSYM (Abbott Laboratories )separately. The results of the seven tested groups were then compared with those of the control, so as to evaluate whether ALP could interfere with the cTnI assay. Results When the chemiluminescent Access cTnI assay was carried out for detection of normal plasma, the concentration of ALP was up to 3 716 U/L and did not interfere with the test results of cTnI [(0. 04 ±0.01) μg/L] compared with those of the control portion [(0. 04 ± 0. 01 ) μg/L] (t = 0. 40, P > 0. 05 ). Once the concentration of ALP went beyond 917 U/L, the AXSYM cTnI assay results [( 0.08 ± 0. 01 ) μg/L] were higher than those of the normal control ( t =-4. 89, P<0. 01 ); When the concentration of ALP was up to 3 534 U/L, the test results of abnormal plasma cTnI detected by the Access assay [( 13.41 ±0. 17) μg/L] did not show significant differences from those of the control [(13.48±0.16) μg/L] (t=0. 52,P>0.05).Conclusions High concentration ofALP did not interfere with the Access cTnI assay or lead to false positive results. However, the high level of ALP( > 917 U/L) could interfere with the AXSYM cTnI assay and cause a false positive result.