高血压杂志
高血壓雜誌
고혈압잡지
2001年
2期
134-137
,共4页
陆利民%孙祥莺%汪军%田德志%郑峪%朱依纯%Thomas Hedner%姚泰
陸利民%孫祥鶯%汪軍%田德誌%鄭峪%硃依純%Thomas Hedner%姚泰
륙이민%손상앵%왕군%전덕지%정욕%주의순%Thomas Hedner%요태
神经肽Y%心肌梗塞%RT-PCR
神經肽Y%心肌梗塞%RT-PCR
신경태Y%심기경새%RT-PCR
目的 观察心肌梗塞后心肌组织中神经肽Y(NPY) Y1受体水平的改变。方法 实验在雄性Sprague-Dawley大鼠中进行，用结扎大鼠冠状动脉左前降支的方法，造成心肌梗塞动物模 型，用RT-PCR方法观察心肌组织中是否存在有NPY Y1受体mRNA，用定量PCR方法观察心肌梗塞后大鼠心肌组织中NPY Y1受体mRNA水平的改变。结果 用RT-PCR方法观察到，大鼠心肌组织中有NPY Y1受体mRNA分布。在大鼠结扎冠状动脉一天及三天后，梗塞区及非梗塞区心肌组织中NPY Y1受体mRNA水平较假手术组大鼠均有显著升高。此外，假手术组大鼠心肌组织中NPY Y1受体mRNA水平较正常大鼠也有显著升高。结论 心肌梗塞可导致梗塞区及非梗塞区心肌组织中NPY Y1受体水平升高，手术创伤应激也可影响心肌组织中NPY Y1受体 水平。
目的 觀察心肌梗塞後心肌組織中神經肽Y(NPY) Y1受體水平的改變。方法 實驗在雄性Sprague-Dawley大鼠中進行，用結扎大鼠冠狀動脈左前降支的方法，造成心肌梗塞動物模 型，用RT-PCR方法觀察心肌組織中是否存在有NPY Y1受體mRNA，用定量PCR方法觀察心肌梗塞後大鼠心肌組織中NPY Y1受體mRNA水平的改變。結果 用RT-PCR方法觀察到，大鼠心肌組織中有NPY Y1受體mRNA分佈。在大鼠結扎冠狀動脈一天及三天後，梗塞區及非梗塞區心肌組織中NPY Y1受體mRNA水平較假手術組大鼠均有顯著升高。此外，假手術組大鼠心肌組織中NPY Y1受體mRNA水平較正常大鼠也有顯著升高。結論 心肌梗塞可導緻梗塞區及非梗塞區心肌組織中NPY Y1受體水平升高，手術創傷應激也可影響心肌組織中NPY Y1受體 水平。
목적 관찰심기경새후심기조직중신경태Y(NPY) Y1수체수평적개변。방법 실험재웅성Sprague-Dawley대서중진행，용결찰대서관상동맥좌전강지적방법，조성심기경새동물모 형，용RT-PCR방법관찰심기조직중시부존재유NPY Y1수체mRNA，용정량PCR방법관찰심기경새후대서심기조직중NPY Y1수체mRNA수평적개변。결과 용RT-PCR방법관찰도，대서심기조직중유NPY Y1수체mRNA분포。재대서결찰관상동맥일천급삼천후，경새구급비경새구심기조직중NPY Y1수체mRNA수평교가수술조대서균유현저승고。차외，가수술조대서심기조직중NPY Y1수체mRNA수평교정상대서야유현저승고。결론 심기경새가도치경새구급비경새구심기조직중NPY Y1수체수평승고，수술창상응격야가영향심기조직중NPY Y1수체 수평。
Aim To investigate the change in neuropeptide Y(NPY) Y1 receptor level in infarcted myocardium tissues of (MI) rats. Method MI was induced by ligating the left descending anterior coronary artery (LAD) in the heart of rats. The techniques of reverse transcription polymerase chain reaction (RT-PCR) were used to identify the exist of NPY Y1 receptor mRNA in myocardial tissues. The technique of semi-quantitative PCR wa s used to observe the change of NPY Y1 receptor mRNA level. Results NPY Y1 receptor mRNA distributed in the heart tissues of rat. Compared with sham operated rats ,the NPY Y1 receptor mRNA level both in infarction area and non-infarction area was increased significantly after MI fo r 1d and 3d. The NPY Y1 receptor mRNA level in the heart tissues of sham-op erated rats was also significantly increased compared with that in control rats . Conclusion These results suggest that MI may result in increase NPY Y1 receptor level in the heart tissues of rats. Stre ss stimulation such as surgery may also increase the NPY Y1 receptor level in the heart.
