CAJ | 학술논문

内皮祖细胞的分离方法有免疫磁珠分离法、淋巴细胞分离液分离法(1.077)和差速贴壁法,这3种方法已被人们广泛使用,均可分离到一定的目的细胞.分离到的目的细胞在培养过程中逐渐分化、成熟、发育为内皮细胞.在内皮细胞和内皮祖细胞的鉴别区分,使用CD34~+/CD133~+/KDR~+鉴定为内皮祖细胞,同时使用内皮祖细胞吞噬Dil-ac-LDLFITC-UEA双阳性的方法也可鉴定为内皮祖细胞.
내피조세포적분리방법유면역자주분리법、림파세포분리액분리법(1.077)화차속첩벽법,저3충방법이피인문엄범사용,균가분리도일정적목적세포.분리도적목적세포재배양과정중축점분화、성숙、발육위내피세포.재내피세포화내피조세포적감별구분,사용CD34~+/CD133~+/KDR~+감정위내피조세포,동시사용내피조세포탄서Dil-ac-LDLFITC-UEA쌍양성적방법야가감정위내피조세포.
There are three isolation methods of endothelial progenitor cells,including immunomagnetic beads,lymphocyte separating medium(1.077)and differential adhesion,which has been widely used and could isolate a certain number of the desired cells.These cells gradually differentiate,mature,and develope into endothelial cells in culture process.In identification,ceils with CD34~+/CD133~+/KDR~+ are identified as endothelial progenitor cells,and at the same time,the double positive phagocytosis assay of Dil-ac-LDLFITC-UEA also help to identify endothelial progenitor cells.