CAJ | 학술논문

植物LRR型类受体蛋白激酶在植物生命活动中发挥着重要作用.前期研究发现,大豆(Glycine max)LRR型类受体蛋白激酶基因GmSARK可能参与调控大豆叶片的衰老过程.利用CaMV 35S启动子驱动组成型过表达GmSA尺,(基因可导致转基因植株出现致死表型,据此构建了可诱导型启动子GVG驱动GmSARK基因过表达的双元表达载体,转化野生型拟南芥(Arabidopsis thaliana)并获得了多株转基因植株.研究结果表明,外源施加诱导物地塞米松可引起GmSARK基因在转基因植株中过表达,并导致转基因植株出现叶片变黄下卷和生长受抑制等表型;外源细胞分裂素处理可以抑制GmSARK的表达.但是不能逆转GmSARK过表达所引起的上述变化.
식물LRR형류수체단백격매재식물생명활동중발휘착중요작용.전기연구발현,대두(Glycine max)LRR형류수체단백격매기인GmSARK가능삼여조공대두협편적쇠로과정.이용CaMV 35S계동자구동조성형과표체GmSA척,(기인가도치전기인식주출현치사표형,거차구건료가유도형계동자GVG구동GmSARK기인과표체적쌍원표체재체,전화야생형의남개(Arabidopsis thaliana)병획득료다주전기인식주.연구결과표명,외원시가유도물지새미송가인기GmSARK기인재전기인식주중과표체,병도치전기인식주출현협편변황하권화생장수억제등표형;외원세포분렬소처리가이억제GmSARK적표체.단시불능역전GmSARK과표체소인기적상술변화.
Lucine-rich-repeat receptor-like kinases (LRR-RLKs) are known to function in various signaling pathways in plants. Our previous study suggested a role for a soybean LRR-RLK, GmSARK, in regulating leaf senescence. Because constitutive expression of GmSARK leads to lethality, we used a dexamethasone (DEX)-inducible transcription system to control the expression of GmSARK in Arabidopsis thaliana. Applying DEX to the transgenic plants effectively induced the overexpression of GmSARK, thus leading to marked growth inhibition and leaf senescence. Interestingly, exogenous cytokinin (6-BA) repressed the expression of GmSARK in the transgenic lines, yet failed to restore the severe phenotypes.