中华危重症医学杂志（电子版）
中華危重癥醫學雜誌（電子版）
중화위중증의학잡지（전자판）
CHINESE JOURNAL OF CRITICAL CARE MEDICINE ( ELECTRONIC EDITON)
2012年
3期
14-17
,共4页
施国英%杨群政%郑翠萍%童向民
施國英%楊群政%鄭翠萍%童嚮民
시국영%양군정%정취평%동향민
原癌基因蛋白质C-MDM2%多发性骨髓瘤%Waldenstrom巨球蛋白血症%印迹法,蛋白质
原癌基因蛋白質C-MDM2%多髮性骨髓瘤%Waldenstrom巨毬蛋白血癥%印跡法,蛋白質
원암기인단백질C-MDM2%다발성골수류%Waldenstrom거구단백혈증%인적법,단백질
Proto-oncogene protein-MDM2%Multiple myeloma%Waldenstrom marcoglobulinemia%Blotting,western
目的 探讨癌基因双微体-2 (MDM2)在多发性骨髓瘤(MM)中的表达及其临床意义.方法 选取2008年7月至2010年6月温州市第二人民医院血液内科MM患者37例,另选5例巨球蛋白血症患者作为疾病对照组,5名骨髓捐献志愿者为健康对照组.应用逆转录聚合酶链式扩增反应和免疫印迹法分别检测各组MDM2mRNA及其蛋白变化.MM患者按国际分期系统将其分为I期11例,Ⅱ期18例,Ⅲ期20例,应用回归分析法观察MDM2 mRNA及其蛋白表达与各分期的相关性.结果 MDM2mRNA及其蛋白在健康对照组表达水平均很低,在疾病对照组表达水平稍高,在MM组则明显表现为过表达(P均＜0.05).另外,MDM2 mRNA及其蛋白表达与MM分期呈正相关(r值分别为0.782、0.821).结论 MDM2基因在MM患者中呈高水平表达,且与MM的临床分期有密切关系,临床上或可作为判断其预后和检测肿瘤微小残留的一个指标.
目的 探討癌基因雙微體-2 (MDM2)在多髮性骨髓瘤(MM)中的錶達及其臨床意義.方法 選取2008年7月至2010年6月溫州市第二人民醫院血液內科MM患者37例,另選5例巨毬蛋白血癥患者作為疾病對照組,5名骨髓捐獻誌願者為健康對照組.應用逆轉錄聚閤酶鏈式擴增反應和免疫印跡法分彆檢測各組MDM2mRNA及其蛋白變化.MM患者按國際分期繫統將其分為I期11例,Ⅱ期18例,Ⅲ期20例,應用迴歸分析法觀察MDM2 mRNA及其蛋白錶達與各分期的相關性.結果 MDM2mRNA及其蛋白在健康對照組錶達水平均很低,在疾病對照組錶達水平稍高,在MM組則明顯錶現為過錶達(P均＜0.05).另外,MDM2 mRNA及其蛋白錶達與MM分期呈正相關(r值分彆為0.782、0.821).結論 MDM2基因在MM患者中呈高水平錶達,且與MM的臨床分期有密切關繫,臨床上或可作為判斷其預後和檢測腫瘤微小殘留的一箇指標.
목적 탐토암기인쌍미체-2 (MDM2)재다발성골수류(MM)중적표체급기림상의의.방법 선취2008년7월지2010년6월온주시제이인민의원혈액내과MM환자37례,령선5례거구단백혈증환자작위질병대조조,5명골수연헌지원자위건강대조조.응용역전록취합매련식확증반응화면역인적법분별검측각조MDM2mRNA급기단백변화.MM환자안국제분기계통장기분위I기11례,Ⅱ기18례,Ⅲ기20례,응용회귀분석법관찰MDM2 mRNA급기단백표체여각분기적상관성.결과 MDM2mRNA급기단백재건강대조조표체수평균흔저,재질병대조조표체수평초고,재MM조칙명현표현위과표체(P균＜0.05).령외,MDM2 mRNA급기단백표체여MM분기정정상관(r치분별위0.782、0.821).결론 MDM2기인재MM환자중정고수평표체,차여MM적림상분기유밀절관계,림상상혹가작위판단기예후화검측종류미소잔류적일개지표.
Objective To investigate the expression and clinical significance of murine double minute 2 (MDM2) in patients with multiple myeloma (MM).Methods Thirty-seven patients with MM,5 with macroglobulinemia (diease control group),and 5 healthy donors (healthy control group) from July 2008 to May 2010 in Wenzhou second people's hospital were enrolled in this study.The MDM2 mRNA and protein were obtained from those groups by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and western blot,respectively,and the results were compared.The patients with MM were further divided into 3groups with different stages according to international staging system,and the correlations between the expressions of MDM2 mRNA and protein and stages were detected by regression analysis.Result The expressions of MDM2 mRNA and protein in the MM group were significantly higher than the two control groups (all P＜ 0.05),and were positively correlated with the MM stages (r =0.782,0.821,respectively).Conclusions The expressions of MDM2 mRNA and protein were significantly up-regulated in patients with MM.They could be used as biomarkers for predicting the prognosis and monitoring minimal residual disease.
