国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
5期
413-415
,共3页
半夏泻心汤%2型糖尿病模型%黑素皮质激素受体4%糖稳态
半夏瀉心湯%2型糖尿病模型%黑素皮質激素受體4%糖穩態
반하사심탕%2형당뇨병모형%흑소피질격소수체4%당은태
BanxiaXiexin decoction%Type 2 diabetes mellitus%Melanin cortical hormone receptor 4%Glucose steady state
目的 探讨半夏泻心汤(BD)对2型糖尿病大鼠糖原合成及垂体黑素皮质激素受体(MC4R)表达的影响以及维持葡萄糖代谢平衡的作用机制.方法 采用SD大鼠建立糖尿病模型,造模成功的大鼠按完全随机法分为糖尿病模型组、二甲双胍组(0.3 g/kg)、BD高剂量组(1.7g/kg)、BD中剂量组(0.85g/kg)、BD低剂量组(0.425 g/kg),另设正常对照组饲以正常饮食,1周后测定各组大鼠空腹体重、垂体MC4R及肝糖原的含量.结果 连续给药1周后,半夏泻心汤高、中、低剂量组大鼠的体重分别为(296.62±1.14)g、(293.83±2.17)g、(286.75±1.91)g,与糖尿病模型组(284.73±2.91)g比较,差异有统计学意义(P＜0.01)；MC4R表达分别为(29.69±1.64) ng/L、(29.38±1.88) ng/L、(30.04±1.24) ng/L,与糖尿病模型组(25.95±2.24) ng/L比较,表达均增强(P均＜0.05)；肝糖原含量分别(15.94±0.82) mg/g、(11.52±0.56) mg/g、(10.48±0.45) mg/g,与糖尿病模型组(9.05±0.44) mg/g比较含量均增加(P＜0.01、P＜0.01、P＜0.05).结论 半夏泻心汤各剂量组通过增加外周组织对糖的利用、提高MC4R表达,调节大鼠摄食行为.
目的 探討半夏瀉心湯(BD)對2型糖尿病大鼠糖原閤成及垂體黑素皮質激素受體(MC4R)錶達的影響以及維持葡萄糖代謝平衡的作用機製.方法 採用SD大鼠建立糖尿病模型,造模成功的大鼠按完全隨機法分為糖尿病模型組、二甲雙胍組(0.3 g/kg)、BD高劑量組(1.7g/kg)、BD中劑量組(0.85g/kg)、BD低劑量組(0.425 g/kg),另設正常對照組飼以正常飲食,1週後測定各組大鼠空腹體重、垂體MC4R及肝糖原的含量.結果 連續給藥1週後,半夏瀉心湯高、中、低劑量組大鼠的體重分彆為(296.62±1.14)g、(293.83±2.17)g、(286.75±1.91)g,與糖尿病模型組(284.73±2.91)g比較,差異有統計學意義(P＜0.01)；MC4R錶達分彆為(29.69±1.64) ng/L、(29.38±1.88) ng/L、(30.04±1.24) ng/L,與糖尿病模型組(25.95±2.24) ng/L比較,錶達均增彊(P均＜0.05)；肝糖原含量分彆(15.94±0.82) mg/g、(11.52±0.56) mg/g、(10.48±0.45) mg/g,與糖尿病模型組(9.05±0.44) mg/g比較含量均增加(P＜0.01、P＜0.01、P＜0.05).結論 半夏瀉心湯各劑量組通過增加外週組織對糖的利用、提高MC4R錶達,調節大鼠攝食行為.
목적 탐토반하사심탕(BD)대2형당뇨병대서당원합성급수체흑소피질격소수체(MC4R)표체적영향이급유지포도당대사평형적작용궤제.방법 채용SD대서건립당뇨병모형,조모성공적대서안완전수궤법분위당뇨병모형조、이갑쌍고조(0.3 g/kg)、BD고제량조(1.7g/kg)、BD중제량조(0.85g/kg)、BD저제량조(0.425 g/kg),령설정상대조조사이정상음식,1주후측정각조대서공복체중、수체MC4R급간당원적함량.결과 련속급약1주후,반하사심탕고、중、저제량조대서적체중분별위(296.62±1.14)g、(293.83±2.17)g、(286.75±1.91)g,여당뇨병모형조(284.73±2.91)g비교,차이유통계학의의(P＜0.01)；MC4R표체분별위(29.69±1.64) ng/L、(29.38±1.88) ng/L、(30.04±1.24) ng/L,여당뇨병모형조(25.95±2.24) ng/L비교,표체균증강(P균＜0.05)；간당원함량분별(15.94±0.82) mg/g、(11.52±0.56) mg/g、(10.48±0.45) mg/g,여당뇨병모형조(9.05±0.44) mg/g비교함량균증가(P＜0.01、P＜0.01、P＜0.05).결론 반하사심탕각제량조통과증가외주조직대당적이용、제고MC4R표체,조절대서섭식행위.
Objective To study the effects of the BanxiaXiexin decoction (BD) on express of glycogen synthesis and the expression of MC4R.and to discuss the mechanism of its maintaining glucose metabolism balance.Methods Diabetic mice were induced and divided into the model group,metformin group(0.3 g/kg)and BD high dose group(1.7 g/kg),medium dose group(0.85 g/kg),and low dose group (0.425 g/kg).In addition,a normal group was set to feed normal diet.Fasting blood glucose,body weight on an empty stomach,express of MC4R and hepatic glycogen were assayed after a week of treatment.Results Body weight of BD high,medium and low dose group was (296.62 ±1.14) g,(293.83 ± 2.1 7 ) g,and (286.75 ± 1.91 ) g respectively,showing a significant increase compared with the model group (284.73± 2.91) g (P＜0.01).MC4R expression of these MD groups were (29.69 ± 1.64) ng/L,(29.38±1.88) ng/L and (30.04 ±1.24) ng/L,showing a significant increase compared with the model group (25.95±2.24) ng/L (P＜0.05).Liver glycogen content of these MD groups were (15.94±0.82)mg/g,(11.52±0.56)mg/g,and (10.48±0.45)mg/g respectively,also showing a significant increase than the model group (9.05±0.44)mg/g(P＜0.01,P＜0.01,P＜0.05).Conclusion BD improves MC4R expression and rat feeding behavior by increasing by peripheral organization utilization of glucose.
