中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2008年
3期
213-216
,共4页
溃疡性结肠炎%多态现象,遗传%MICB基因
潰瘍性結腸炎%多態現象,遺傳%MICB基因
궤양성결장염%다태현상,유전%MICB기인
Colitis,ulcerative%Polymorphism%MHC class Ⅰ chain-related gene-B
目的 MHC Ⅰ类相关基因(MHC class Ⅰ chain-related gene,MIC)与MHC Ⅰ类基因连锁,同源性很高.我们的前期研究发现MICA和MICB微卫星多态性与溃疡性结肠炎(UC)相关,本研究继续探讨MICB第2、3、4外显子基因多态性与UC的相关性,以期发现UC的易感基因.方法 采用聚合酶链反应-单链构象多态性方法(PCR-SSCP),对105例湖北汉族UC患者和213例正常对照者进行MICB基因分型.结果 UC患者的MICB 0106等位基因频率较正常组显著增高(19.0%比8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI为1.488～3.879).在临床亚型分析中,MICB 0106等位基因频率在全结肠炎、中重度UC组及有肠外表现的UC患者中均显著增高(分别为24.4%比8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI为1.800～6.027;24.1%比8.9%,P=0.000,Pc＜0.001,OR=3.249,95%CI为1.893～5.576;20.5%比8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI为1.418～4.861).而且MICB 0106等位基因频率在男性和年龄≥40岁的患者中比正常组显著增高(分别为22.1%比8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI为1.737～6.178;28.8%比8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI为2.381～8.504).结论 MICB 0106等位基因与湖北汉族人UC存在显著相关性,而且与全结肠炎、中重度UC、肠外表现、男性及年龄≥40岁的患者有关,提示携带MICB 0106等位基因的个体对UC存在遗传易感性.
目的 MHC Ⅰ類相關基因(MHC class Ⅰ chain-related gene,MIC)與MHC Ⅰ類基因連鎖,同源性很高.我們的前期研究髮現MICA和MICB微衛星多態性與潰瘍性結腸炎(UC)相關,本研究繼續探討MICB第2、3、4外顯子基因多態性與UC的相關性,以期髮現UC的易感基因.方法 採用聚閤酶鏈反應-單鏈構象多態性方法(PCR-SSCP),對105例湖北漢族UC患者和213例正常對照者進行MICB基因分型.結果 UC患者的MICB 0106等位基因頻率較正常組顯著增高(19.0%比8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI為1.488～3.879).在臨床亞型分析中,MICB 0106等位基因頻率在全結腸炎、中重度UC組及有腸外錶現的UC患者中均顯著增高(分彆為24.4%比8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI為1.800～6.027;24.1%比8.9%,P=0.000,Pc＜0.001,OR=3.249,95%CI為1.893～5.576;20.5%比8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI為1.418～4.861).而且MICB 0106等位基因頻率在男性和年齡≥40歲的患者中比正常組顯著增高(分彆為22.1%比8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI為1.737～6.178;28.8%比8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI為2.381～8.504).結論 MICB 0106等位基因與湖北漢族人UC存在顯著相關性,而且與全結腸炎、中重度UC、腸外錶現、男性及年齡≥40歲的患者有關,提示攜帶MICB 0106等位基因的箇體對UC存在遺傳易感性.
목적 MHC Ⅰ류상관기인(MHC class Ⅰ chain-related gene,MIC)여MHC Ⅰ류기인련쇄,동원성흔고.아문적전기연구발현MICA화MICB미위성다태성여궤양성결장염(UC)상관,본연구계속탐토MICB제2、3、4외현자기인다태성여UC적상관성,이기발현UC적역감기인.방법 채용취합매련반응-단련구상다태성방법(PCR-SSCP),대105례호북한족UC환자화213례정상대조자진행MICB기인분형.결과 UC환자적MICB 0106등위기인빈솔교정상조현저증고(19.0%비8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI위1.488～3.879).재림상아형분석중,MICB 0106등위기인빈솔재전결장염、중중도UC조급유장외표현적UC환자중균현저증고(분별위24.4%비8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI위1.800～6.027;24.1%비8.9%,P=0.000,Pc＜0.001,OR=3.249,95%CI위1.893～5.576;20.5%비8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI위1.418～4.861).이차MICB 0106등위기인빈솔재남성화년령≥40세적환자중비정상조현저증고(분별위22.1%비8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI위1.737～6.178;28.8%비8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI위2.381～8.504).결론 MICB 0106등위기인여호북한족인UC존재현저상관성,이차여전결장염、중중도UC、장외표현、남성급년령≥40세적환자유관,제시휴대MICB 0106등위기인적개체대UC존재유전역감성.
Objecfive To investigate the association between the exon 2,3,4 of MHC class Ⅰ chain-related gene-B(MICB)and ulcerative colitis(UC)in Chinese Han.Methods Using polymerase chain reaction single-stranded conformation polymorphism,allele frequency of MICB exon 2,3 and 4 in 105 patients with UC and 213 age and sex matched healthy controls were genotyped.All of the studied individuals were Chinese Han.Results Allele frequency of MICB 0106 was increased in patients with UC as compared with normal controls(19.0%vs 8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI:1.488-3.879).The frequency of MICB 0106 was increased significantly in patients with extensive colitis (24.4%vs 8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI:1.800-6.027),moderate and severe disease(24.1%vs 8.9%,P=0.000.Pc＜0.001,OR=3.294 95%CI:1.893-5.576)and in those with extra intestinal manifestations(20.5%vs 8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI:1.418-4.861).Furthermore,MICB 0106 allele was higher in frequency in the male patients with UC (22.1%vs 8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI:1.737-6.178)and the patients more than 40 years old(28.8%vs 8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI:2.381-8.504)as compared with healthy controls.Conclusion MICB 0106 allele is positively associated with UC,especially with extensive colitis,moderate and severe disease,presence of extra intestinal manifestations,male gender and age of more than 40 years in Chinese Han in Hubei province.
