CAJ | 학술논문

目的探讨超声微泡靶向破坏介导下HSV-TK基因联合更昔洛韦对裸鼠卵巢癌的杀伤效应.方法挑选成功种植卵巢癌细胞且状态良好的雌性BALB/c-nu小鼠40只,随机分为4组:A组,HSV-TK+超声微泡(MBs)+超声辐射(US)组；B组,HSV-TK+超声辐射(US)组；C组,HSV-TK组；D组,磷酸盐缓冲液(PBS)组.分别利用Western-Blot和real time RT-PCR检测各组瘤体组织内TK蛋白和mRNA的表达,TUNEL法检测不同组的肿瘤细胞凋亡,并比较各组之间的肿瘤生长状况及裸鼠生存时间.结果 A组TK蛋白、mRNA表达及肿瘤细胞凋亡率高于其他各组(P＜0.05),平均肿瘤体积A组显著低于其他各组(P＜0.05),但生存时间上A组同其他各组之间差异并无统计学意义.结论超声微泡靶向破坏介导HSV-TK联合更昔洛韦可促进裸鼠卵巢癌细胞的凋亡,并抑制肿瘤生长速度,但短期观察并不能延长裸鼠生存时间.
목적 탐토초성미포파향파배개도하HSV-TK기인연합경석락위대라서란소암적살상효응.방법 도선성공충식란소암세포차상태량호적자성BALB/c-nu소서40지,수궤분위4조:A조,HSV-TK+초성미포(MBs)+초성복사(US)조；B조,HSV-TK+초성복사(US)조；C조,HSV-TK조；D조,린산염완충액(PBS)조.분별이용Western-Blot화real time RT-PCR검측각조류체조직내TK단백화mRNA적표체,TUNEL법검측불동조적종류세포조망,병비교각조지간적종류생장상황급라서생존시간.결과 A조TK단백、mRNA표체급종류세포조망솔고우기타각조(P＜0.05),평균종류체적A조현저저우기타각조(P＜0.05),단생존시간상A조동기타각조지간차이병무통계학의의.결론 초성미포파향파배개도HSV-TK연합경석락위가촉진라서란소암세포적조망,병억제종류생장속도,단단기관찰병불능연장라서생존시간.
Objective To investigate the anti-tumor effect of ultrasound-targeted microbubble destruction (UTMD) mediated herpes simplex virus thymedine kinase (HSV-TK) on mice ovarian cancer.Methods Forty female BALB/c-nu mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were established:(A)HSV-TK + Microbubbles + Ultrasound (HSV-TK+ MBs + US);(B) HSV-TK+ Ultrasound (HSV-TK + US);(C) HSV-TK;(D) PBS.TheTK protein and mRNA expression were separately detected by western-blot and real time RT-PCR.TUNEL staining was used to detect the tumor cell apoptosis.The inhibition rates and survival time of the animals were compared among all groups.Results The HSV-TK gene transfection efficacy and tumor inhibitory effect of HSV-TK on mice transplantable tumor in group A (HSV-TK + MBs + US) were significantly improved compared with group B (HSV-TK + US),group C (HSV-TK) and group D (PBS) (P ＜0.05).However,group A has no significant difference (P ＞0.05) with other groups in improving the survival time of tumor-bearing mice.Conclusions Ultrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on ovarian cancer in mice.However,this new method is not able to improve the survival time of mice for a short-term observation.