中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
3期
225-229
,共5页
糖尿病%阿司匹林%培哚普利%泼尼松%心肌病变
糖尿病%阿司匹林%培哚普利%潑尼鬆%心肌病變
당뇨병%아사필림%배타보리%발니송%심기병변
Diabetes%Aspirin%Perindopril%Prednisone%Cardiac pathology
目的 探讨1型糖尿病大鼠心肌病理改变及阿司匹林、培哚普利、泼尼松对其心肌病变的影响.方法 30只SD大鼠随机分为糖尿病模型组(n=20)和正常对照组(n=10),糖尿病模型组一次性腹腔注射链脲佐菌素(STZ)60 mg/kg加枸橼酸钠溶液,正常对照组腹腔注射枸橼酸钠溶液.8周后观察2组大鼠的体重和平均血糖浓度.将建模的20只糖尿病大鼠随机分为4组(n=5),第4天起,分别给予生理盐水溶解的阿司匹林、培哚普利、泼尼松灌胃各5只,余5只和正常对照组每日以等体积生理盐水灌胃,共持续8周.8周后取各组大鼠心肌组织,HE染色,光镜和电镜下进行观察和比较.结果 大鼠以STZ注射后3 d血糖浓度≥16.7 mmol/L为造模成功,模型组20只大鼠全部成活,成模后大鼠出现明显多饮、多食、多尿和体重下降,HE染色显示糖尿病大鼠心肌细胞肿胀肥大、变性,核明显增大,心肌纤维断裂、溶解,收缩呈波浪状,排列紊乱,并有炎性细胞的浸润.电镜下可见糖尿病大鼠心肌肌纤维排列不规则,断裂溶解,肌纤维间糖原聚集,线粒体肿胀,线粒体大部分嵴不清晰,细胞间盘增宽.与糖尿病组相比,各药物干预组上述病理改变均有所减轻,而以培哚普利组最为明显.结论 STZ诱导的1型糖尿病大鼠模型制作方法成功率高,血糖值稳定,8周后出现明显的心肌病变,阿司匹林、培哚普利、泼尼松均能使糖尿病大鼠心肌病变有所减轻,以培哚普利最为有效.
目的 探討1型糖尿病大鼠心肌病理改變及阿司匹林、培哚普利、潑尼鬆對其心肌病變的影響.方法 30隻SD大鼠隨機分為糖尿病模型組(n=20)和正常對照組(n=10),糖尿病模型組一次性腹腔註射鏈脲佐菌素(STZ)60 mg/kg加枸櫞痠鈉溶液,正常對照組腹腔註射枸櫞痠鈉溶液.8週後觀察2組大鼠的體重和平均血糖濃度.將建模的20隻糖尿病大鼠隨機分為4組(n=5),第4天起,分彆給予生理鹽水溶解的阿司匹林、培哚普利、潑尼鬆灌胃各5隻,餘5隻和正常對照組每日以等體積生理鹽水灌胃,共持續8週.8週後取各組大鼠心肌組織,HE染色,光鏡和電鏡下進行觀察和比較.結果 大鼠以STZ註射後3 d血糖濃度≥16.7 mmol/L為造模成功,模型組20隻大鼠全部成活,成模後大鼠齣現明顯多飲、多食、多尿和體重下降,HE染色顯示糖尿病大鼠心肌細胞腫脹肥大、變性,覈明顯增大,心肌纖維斷裂、溶解,收縮呈波浪狀,排列紊亂,併有炎性細胞的浸潤.電鏡下可見糖尿病大鼠心肌肌纖維排列不規則,斷裂溶解,肌纖維間糖原聚集,線粒體腫脹,線粒體大部分嵴不清晰,細胞間盤增寬.與糖尿病組相比,各藥物榦預組上述病理改變均有所減輕,而以培哚普利組最為明顯.結論 STZ誘導的1型糖尿病大鼠模型製作方法成功率高,血糖值穩定,8週後齣現明顯的心肌病變,阿司匹林、培哚普利、潑尼鬆均能使糖尿病大鼠心肌病變有所減輕,以培哚普利最為有效.
목적 탐토1형당뇨병대서심기병리개변급아사필림、배타보리、발니송대기심기병변적영향.방법 30지SD대서수궤분위당뇨병모형조(n=20)화정상대조조(n=10),당뇨병모형조일차성복강주사련뇨좌균소(STZ)60 mg/kg가구연산납용액,정상대조조복강주사구연산납용액.8주후관찰2조대서적체중화평균혈당농도.장건모적20지당뇨병대서수궤분위4조(n=5),제4천기,분별급여생리염수용해적아사필림、배타보리、발니송관위각5지,여5지화정상대조조매일이등체적생리염수관위,공지속8주.8주후취각조대서심기조직,HE염색,광경화전경하진행관찰화비교.결과 대서이STZ주사후3 d혈당농도≥16.7 mmol/L위조모성공,모형조20지대서전부성활,성모후대서출현명현다음、다식、다뇨화체중하강,HE염색현시당뇨병대서심기세포종창비대、변성,핵명현증대,심기섬유단렬、용해,수축정파랑상,배렬문란,병유염성세포적침윤.전경하가견당뇨병대서심기기섬유배렬불규칙,단렬용해,기섬유간당원취집,선립체종창,선립체대부분척불청석,세포간반증관.여당뇨병조상비,각약물간예조상술병리개변균유소감경,이이배타보리조최위명현.결론 STZ유도적1형당뇨병대서모형제작방법성공솔고,혈당치은정,8주후출현명현적심기병변,아사필림、배타보리、발니송균능사당뇨병대서심기병변유소감경,이배타보리최위유효.
Objective To investigate the pathological changes of myocardium in diabetic rats and the effect of aspirin, perindopril, prednisone on the pathological changes of myocardium in type 1 diabetic rats. Methods Thirty SD rats were randomly divided into the following groups: ( 1 ) diabetes mellitus (DM)group ( n = 20): treated with intraperitoneal injection of streptozocin (STZ) 60 mg/kg at one time, diluted in sodium citrate buffer. (2) normal control group (n = 10): intraperitoneal injection of sodium citrate buffer. The blood glucose and the weight were measured regularly for 8 weeks. The 20 diabetic model rats were randomly divided into four groups (n = 5 individually), from the 4th day, the rats were given normal saline dissolved aspirin, perindopril, prednisone gavage respectively, and the normal control group and diabetic group were given an equal volume of saline daily gavage. After 8 weeks, the pathological changes in the cardiac muscle tissue were observed under light microscope and electron microscope. Results Three days after the injection of STZ, the blood glucose in the DM group was higher than 16. 7 mmol/L,20 rats were all modeling successfully. The model rats suffered from polydipsia, polyphagia, polyuria and weight loss significantly. HE staining showed diabetic myocardial cell swell, hypertrophy, degeneration and significant nuclear enlargement; the myocardial fiber fractured,dissolved, contraction wavy and disorganized fibre infiltrated by inflammatory cells. Under electron microscope, diabetic myocardial muscle fibers arranged irregularly, fractured and dissolved; the glycogen between the muscle fiber accumulated, mitochondria swelled, most of the ridges in mitochondria were not clear, cell plate widened. Compared with the diabetic group, the above-mentioned pathological changes reduced in the drug intervention group,and the changes in perindopril group was most significant. Conclusion Injection of STZ 60 mg/kg could successfully induce diabetic in rats and held stable blood glucose level. After 8 weeks, diabetes could cause servere damages in myocardium in the rats, but aspirin, perindopril, prednisone could reduce the cardiomyopathy in diabetic rats,in which perindopril had more positive effect.
